Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Perera, Ishara; Fukushima, Ayaka; Akabane, Tatsuki; Horiguchi, Genki; Seneweera, Saman; Hirotsu, Naoki

doi:10.1038/s41598-019-51485-2

Cited by 12 publications

(15 citation statements)

References 45 publications

(59 reference statements)

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“…Phytate and other cations are stored in the protein storage vacuole (PSV) within small, crystalline storage bodies, known as globoids [9,15]. While the location of globoids varies across species, a majority of these are found within the aleurone layer of cereal crops, such as wheat and barley, as well as oilseed crops including peanuts, hemp, sunflower, and cotton [6,9,16]. Maize globoids are concentrated within the germ, whereas soybean phytate is distributed throughout the entire seed [6,17,18].…”

Section: Introductionmentioning

confidence: 99%

Can Inositol Pyrophosphates Inform Strategies for Developing Low Phytate Crops?

Freed

Adepoju

Gillaspy

2020

Plants

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Inositol pyrophosphates (PP-InsPs) are an emerging class of “high-energy” intracellular signaling molecules, containing one or two diphosphate groups attached to an inositol ring, that are connected with phosphate sensing, jasmonate signaling, and inositol hexakisphosphate (InsP6) storage in plants. While information regarding this new class of signaling molecules in plants is scarce, the enzymes responsible for their synthesis have recently been elucidated. This review focuses on InsP6 synthesis and its conversion into PP-InsPs, containing seven and eight phosphate groups (InsP7 and InsP8). These steps involve two types of enzymes: the ITPKs that phosphorylate InsP6 to InsP7, and the PPIP5Ks that phosphorylate InsP7 to InsP8. This review also considers the potential roles of PP-InsPs in plant hormone and inorganic phosphate (Pi) signaling, along with an emerging role in bioenergetic homeostasis. PP-InsP synthesis and signaling are important for plant breeders to consider when developing strategies that reduce InsP6 in plants, as this will likely also reduce PP-InsPs. Thus, this review is primarily intended to bridge the gap between the basic science aspects of PP-InsP synthesis/signaling and breeding/engineering strategies to fortify foods by reducing InsP6.

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Section: Introductionmentioning

confidence: 99%

Can Inositol Pyrophosphates Inform Strategies for Developing Low Phytate Crops?

Freed

Adepoju

Gillaspy

2020

Plants

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“…This suggests that the biosynthesized PA using influxed Pi determines the PA response to P application. To assess the PA biosynthesis level, we analyzed INO1 gene expression, which we previously reported as the key determinant that explains genotypic differences between WRC 5 and WRC 6 [32]. The expression of INO1 differed with genotype; WRC 6 responded to P fertilizer, whereas WRC 5 did not show any response to P fertilizer (Figure 4).…”

Section: Effect Of P Fertilizer On Pa Biosynthesismentioning

confidence: 98%

“…The biosynthesis of PA continues until 25 DAF [32,34]; we investigated the expression level of other genes related to PA synthesis identified from the Nipponbare genome database [35]. Seven genes, namely, inositol 1, 3, 4-trisphosphate 5/6-kinase 2 (ITPK2), 2-phospho-glycerate kinase (2-PGK), inositol-pentakisphosphate 2-kinase 1 (IPK1), myo-inositol kinase (MIK), inositol 1, 3, 4-trisphoshate 5/6-kinase 2 (ITPK6), inositol 1, 3, 4-triskisphosphate 5/6-kinase 1 (ITPK1), and INO1 showed no response to P fertilizer treatment in Nipponbare ( Figure 5).…”

Section: Other Factors Regulating Grain Pa Contentmentioning

confidence: 99%

“…We then compared WRC 5 and WRC 6 to identify the molecular determinant of the natural variation in the PA content in rice. The results suggested the gene expression level of myo-inositol 3-phosphate synthase 1 (INO1) was the genetic basis explaining the natural variation in PA accumulation in rice [32]. Interestingly, DNA sequences of the coding and promoter region (1000 bp) of the INO1 gene were identical between WRC 5 and WRC 6.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic Differences in the Effect of P Fertilization on Phytic Acid Content in Rice Grain

Fukushima

Perera²,

Hosoya

et al. 2020

Plants

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Phytic acid (PA) prevents the absorption of minerals in the human intestine, and it is regarded as an antinutrient. Low PA rice is beneficial because of its higher Zn bioavailability and it is suggested that the gene expression level of myo-inositol 3-phosphate synthase 1 (INO1) in developing grain is a key factor to explain the genotypic difference in PA accumulation among natural variants of rice. P fertilization is also considered to affect the PA content, but it is not clear how it affects INO1 gene expression and the PA content in different genotypes. Here, we investigated the effect of P fertilization on the PA content in two contrasting rice genotypes, with low and high PA accumulation, respectively. Based on the results of the analysis of the PA content, inorganic P content, INO1 gene expression, and xylem sap inorganic P content, we concluded that the effect of P fertilization on PA accumulation in grain differed with the genotype, and it was regulated by multiple mechanisms.

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“…Therefore, reducing PA content without the need for polishing out the aleurone layer is an efficient way to improve mineral bioavailability in rice. Although, the genetic and environmental factors controlling P content in rice have been investigated, including P fertilizers, there is still not enough information to help understand the dynamics of P contents in brown rice [ 15 , 16 ]. Low phytic acid ( lpa ) mutant rice has been developed to increase mineral bioavailability [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Phytic Acid in Brown Rice Can Be Reduced by Increasing Soaking Temperature

Fukushima

Uchino

Akabane

et al. 2020

Foods

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Phytic acid (PA) is a storage form of phosphorus in seeds. Phytase enzyme is activated at germination and hydrolyses PA into myo-inositol and inorganic phosphate. PA inhibits the absorption of minerals in the human intestine by chelation. Its degradation, therefore, is a key factor to improve mineral bioavailability in rice. Germinated brown rice (GBR) is favoured because it improves the availability of nutrients, and thus have a positive effect on health. In this study, we show the effects of soaking temperature on phytase activity and PA content in GBR. Rice phytase showed thermostability and its activity peaked at 50 °C. After 36 h of soaking, phytase activity was significantly increased at 50 °C and PA content was significantly decreased, compared to that at 30 °C. Zinc (Zn) analysis revealed that there was no significant difference in Zn content among different temperature treatments. Calculated total daily absorbed Zn (TAZ) was significantly higher in GBR compared with non-soaked seeds. Moreover, brown rice grains germinated at 50 °C showed a higher TAZ value than that at 30 °C. Seed germination and seed water soaking at high temperatures reduce PA content in brown rice showing a potentially effective way to improve mineral bioavailability in brown rice.

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Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Cited by 12 publications

References 45 publications

Can Inositol Pyrophosphates Inform Strategies for Developing Low Phytate Crops?

Can Inositol Pyrophosphates Inform Strategies for Developing Low Phytate Crops?

Genotypic Differences in the Effect of P Fertilization on Phytic Acid Content in Rice Grain

Phytic Acid in Brown Rice Can Be Reduced by Increasing Soaking Temperature

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