Expression signatures of long non-coding RNAs in early brain injury following experimental subarachnoid hemorrhage

Abstract

Subarachnoid hemorrhage (SAH) is an important cause of mortality in stroke patients. Long non-coding RNAs (LncRNAs) have important functions in brain disease, however their expression profiles in SAH remain to be elucidated. The present study aimed to investigate the expression signatures of LncRNAs and mRNAs in early brain injury (EBI) following SAH in a rat model. Male Wistar rats were randomly divided into an SAH group and a sham operation group. The expression signatures of the LncRNAs and mRNAs in the temporal lobe cortex were investigated using a rat LncRNAs array following experimental SAH. The results revealed that there were 144 downregulated and 64 upregulated LncRNAs and 181 downregulated and 221 upregulated mRNAs following SAH. Additionally, two upregulated (BC092207, MRuc008hvl) and three downregulated (XR_006756, MRAK038897, MRAK017168) LncRNAs were confirmed using reverse transcription quantitative polymerase chain reaction. The differentially expressed mRNAs were further analyzed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The pathway analysis results provided by the KEGG database indicated that eight pathways associated with inflammation were involved in EBI following SAH. In conclusion, these results demonstrated that the expression profiles of the LncRNAs and mRNAs were significantly different between the SAH-induced EBI group and the sham operation group. These differently expressed LncRNAs may be important in EBI following SAH.

Non-coding RNAs and neuroprotection after acute CNS injuries

“…While Adm activates pro-inflammatory cytokines, BMP7 prevents axonal growth, α-syn promotes post-SCI neuronal death and Wnt3 is essential for neural regeneration after SCI (Zeng et al, 2014b; Matsuura et al, 2008; Busch and Morgan, 2012; Yin et al, 2008). SAH was also shown to significantly alter the lncRNA expression profiles in adult rat brain (Zheng et al, 2015). Bioinformatics analysis of their co-expressed mRNAs indicated that pathways associated with inflammation, neuroactive ligand-receptor interaction, calcium signaling and antigen processing and presentation were probably controlled by these lncRNAs in promoting early brain injury following SAH (Zheng et al, 2015).…”

“…SAH was also shown to significantly alter the lncRNA expression profiles in adult rat brain (Zheng et al, 2015). Bioinformatics analysis of their co-expressed mRNAs indicated that pathways associated with inflammation, neuroactive ligand-receptor interaction, calcium signaling and antigen processing and presentation were probably controlled by these lncRNAs in promoting early brain injury following SAH (Zheng et al, 2015). …”

The Role of Long Non-Coding RNAs in Intracranial Aneurysms and Subarachnoid Hemorrhage

“…In the brain tissues of a rat SAH model, a microarray study indicated that 402 mRNAs and 208 lncRNAs were significantly dysregulated. Among these dysregulated mRNAs and lncRNAs, 221 upregulated and 181 downregulated mRNAs and 64 upregulated and 144 downregulated lncRNAs [45]. Furthermore, five lncRNAs (BC092207, MRuc008hvl, XR_006756, MRAK038897, and MRAK017168) were confirmed by qRT-PCR, where the expression levels of BC092207 and MRuc008hvl were significantly upregulated and the expression levels of XR_006756, MRAK038897, and MRAK017168 were markedly downregulated.…”

Microarray Analysis Reveals a Potential Role of lncRNA Expression in 3,4-Benzopyrene/Angiotensin II-Activated Macrophage in Abdominal Aortic Aneurysm

“…GO and KEGG pathway analyses were performed by KangChen Bio-tech Co. Ltd. (Shanghai, China), in order to analyze the differentially expressed genes systematically and enrich significant GO terms and KEGG pathways ( p < 0.05) [ 15 ]. The significance of the p value was evaluated by the false discovery rate (FDR), and an FDR < 0.05 was recommended.…”