Expression Status and Prognostic Values of SOCS Family Genes in Non-small Cell Lung Cancer

Zhang, Xuede; Sun, Kai; Bao, Lingling

doi:10.21203/rs.3.rs-583442/v1

Cited by 9 publications

(13 citation statements)

References 64 publications

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“…Slc25a24, Cnpy2 and BAX overexpression was reported in breast cancer 66 . Similar to our work, a recent study reported on the overexpression of Plin3 in the breast cancer tissues in comparison with healthy breast 67 . HMGB1 is considered a ubiquitous protein, which has a role as a nuclear cofactor in the regulation of transcription 68 .…”

Section: Discussionsupporting

confidence: 92%

Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo

Vitkin

Singh

Wise

et al. 2022

Sci Rep

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Excision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of solid tumors. Here we introduce e-biopsy—a novel concept for molecular profiling of solid tumors using molecular sampling with electroporation. As e-biopsy provides access to the molecular composition of a solid tumor by permeabilization of the cell membrane, it facilitates tumor diagnostics without tissue resection. Furthermore, thanks to its non tissue destructive characteristics, e-biopsy enables probing the solid tumor multiple times in several distinct locations in the same procedure, thereby enabling the spatial profiling of tumor molecular heterogeneity.We demonstrate e-biopsy in vivo, using the 4T1 breast cancer model in mice to assess its performance, as well as the inferred spatial differential protein expression. In particular, we show that proteomic profiles obtained via e-biopsy in vivo distinguish the tumors from healthy breast tissue and reflect spatial tumor differential protein expression. E-biopsy provides a completely new molecular sampling modality for solid tumors molecular cartography, providing information that potentially enables more rapid and sensitive detection at lesser risk, as well as more precise personalized medicine.

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Section: Discussionsupporting

confidence: 92%

Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo

Vitkin

Singh

Wise

et al. 2022

Sci Rep

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“…This protein mediates a connection between LD and mitochondria and presumably displays a cytoprotective effect via decreasing fatty acid toxicity (reviewed in Kimmel and Sztalryd 2014). PLINs have been shown as suitable markers for LDs in human tumors (Zhang et al 2018(Zhang et al , 2020(Zhang et al , 2021. In breast cancer, high expression of PLIN1 predicted a longer overall patient survival, while overexpression of PLIN2 indicated poor overall patient survival (Zhang et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…PLINs have been shown as suitable markers for LDs in human tumors (Zhang et al 2018(Zhang et al , 2020(Zhang et al , 2021. In breast cancer, high expression of PLIN1 predicted a longer overall patient survival, while overexpression of PLIN2 indicated poor overall patient survival (Zhang et al 2021). Determining the respective PLIN proteins aided the differentiation of tumor types in liposarcomas (Zhang et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models

et al. 2022

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Lipid droplets were identified as important players in biological processes of various tumor types. With emphasis on lipid droplet-coating proteins (perilipins, PLINs), this study intended to shed light on the presence and formation of lipid droplets in canine osteosarcoma. For this purpose, canine osteosarcoma tissue samples (n = 11) were analyzed via immunohistochemistry and electron microscopy for lipid droplets and lipid droplet-coating proteins (PLINs). Additionally, we used the canine osteosarcoma cell lines D-17 and COS4288 in 2D monolayer and 3D spheroid (cultivated for 7, 14, and 21 days) in vitro models, and further analyzed the samples by means of histochemistry, immunofluorescence, molecular biological techniques (RT-qPCR, Western Blot) and electron microscopical imaging. Lipid droplets, PLIN2, and PLIN3 were detected in osteosarcoma tissue samples as well as in 2D and 3D cultivated D-17 and COS4288 cells. In spheroids, specific distribution patterns of lipid droplets and perilipins were identified, taking into consideration cell line specific zonal apportionment. Upon external lipid supplementation (oleic acid), a rise of lipid droplet amount accompanied with an increase of PLIN2 expression was observed. Detailed electron microscopical analyzes revealed that lipid droplet sizes in tumor tissue were comparable to that of 3D spheroid models. Moreover, the biggest lipid droplets were found in the central zone of the spheroids at all sampling time-points, reaching their maximum size at 21 days. Thus, the 3D spheroids can be considered as a relevant in vitro model for further studies focusing on lipid droplets biology and function in osteosarcoma.

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“…And as one of the redox-dependent factors of tumor, PLIN5 indirectly affected the redox state of adipose tissue in GC ( 34 ), which was potentially related to the prognosis of patients with GC. Additionally, the prognostic value of PLIN5 has been explored in many cancers, including breast cancer ( 35 ), pancreatic cancer ( 24 ), hepatocellular carcinoma ( 25 ), and so on; however, we first investigated the prognostic role of mutated PLIN5 in patients with GC. All the above evidence indicated that ELP6 and PLIN5 mutations were probably prognostic biomarkers for patients with GC.…”

Section: Discussionmentioning

confidence: 99%

ELP6 and PLIN5 Mutations Were Probably Prognostic Biomarkers for Patients With Gastric Cancer

Chai

Yang

et al. 2022

Front. Med.

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PurposeGastric cancer (GC) is the fifth leading cancer around world. And prognosis of patients with GC is still undesirable. Our study aimed to explore potential prognostic biomarkers for patients with GC.MethodsThe clinical samples were collected from the Qinghai University Affiliated Hospital, which were subjected to the whole exome sequencing (WES). The other GC-related data were obtained from The Cancer Genome Atlas (TCGA) database. Cross analyses were done to determine the candidate genes. And the final mutated genes were determined by survival analyses, univariate and multivariate Cox regression analyses. CIBERSORT and GSEA were used for immune cell infiltration analysis and functional enrichment, respectively.ResultsAfter cross analyses, 160 candidate-mutated genes were identified. And mutated ELP6 and PLIN5 were significantly independently correlated with the overall survival (OS) of patients with GC. Patients with GC with ELP6 and PLIN5 mutations had worse and better prognosis, respectively. Totally 5 types of immune cells were significantly differentially infiltrated in wild-type and mutated ELP6 and PLIN5 GC samples. In mutated ELP6 and PLIN5 GC samples, totally 7 and 11 pathways were significantly enriched, respectively.ConclusionsThe ELP6 and PLIN5 mutations were probably prognostic biomarkers for patients with GC.

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Expression Status and Prognostic Values of SOCS Family Genes in Non-small Cell Lung Cancer

Cited by 9 publications

References 64 publications

Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo

Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo

Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models

ELP6 and PLIN5 Mutations Were Probably Prognostic Biomarkers for Patients With Gastric Cancer

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