Expressions of CD274 (PD-L1) and CD47 rReceptors on the sSurface of bBlast cCells in AML pPatients

Abstract

Acute myeloid leukemia (AML) is a genetically heterogeneous clonal disease defined by the proliferation and accumulation of immature hematopoietic cells in the bone marrow and blood. This study aimed to evaluate the CD47 and CD274 (PD-L1) expression in Iraqi AML patients and its role in the disease and evasion of malignant cells from immune system. The study was conducted on 85 patients diagnosed with leukemia, 25 of them were excluded as they had taken chemotherapy for less than a week and because the proportion of tumor cells was less than 20%. Virtually, it was conducted on 60 patients with AML and 20 healthy individuals as a control group. The expression of CD47 and CD274 (PD-L1) was evaluated using flow cytometry in the peripheral blood of AML samples only. Also MMP-2 level in serum was evaluated via ELISA in both AML and control samples. The expression of CD47 and CD274 in AML patients was found to be high. Analysis of CD274 (PDL1) expression in blast populations showed a significant increase in the proportion of CD274 (PD-L1) positive blast cells in isolated peripheral blood samples (66.3050 ± 1.94522). Also, the CD47 expression on blast cells in AML was recorded as significantly high (64.9333±2.05873). For MMP-2 (ng/ml) level in serum, the mean level non-significantly increased in AML as compared to the control group (102.677±8.719; 92.191±5.161) respectively.

Defining the T cell transcriptional landscape in pediatric liver transplant rejection at single cell resolution

“…Interactions between PTPRC + KC/Macrophage subpopulations or CD1c + KC/Macrophage subpopulations with unexpanded and unexpanded CD8 NK-like T cell subpopulations which were largely inhibitory (LILRB1/2, LGALS9, FASLG, TGFB1-TGFBR3, LGALS9-CD47; Figure 8B). These inhibitory interactions may serve to constrain activation and proliferation of CD8 NK-like T cells in the liver allograft, as CD47 is an immune checkpoint inhibitor which delivers a "don't eat me" signal to macrophages (57) and is required for acceptance of hepatocyte transplants in the mouse (58,59). In contrast, interactions between KC/Macrophage subpopulations and expanded CD8 EFF were primarily activating.…”

“…Similarly, interactions between CD8 EXP NK-like T cells and the CD1C + KC/macrophage subpopulation uniquely identified LGALS9-CD47, an immune checkpoint inhibitor, as a predicted receptor-ligand pair (57)(58)(59). In contrast, interactions between KC/Macrophage subpopulations and expanded CD8 EFF were primarily activating, with CD160-TNFRSF14 (LIGHT), ALOX5-ALOX5AP (lipoxygenase pathway), and LTB-LTBR receptor-ligand interactions occurred specifically between all five KC/macrophage subpopulations and CD8 EXP T cells, but not between KC/macrophage subpopulations and CD8 UNEXP T cells (Figure 8B).…”

Response of maize grain yield and components to foliar iron nanoparticle application

“…• Total grain yield (ton. ha -1 ): extracted from the yield of individual plants yield x number of plants/hectare and weight-adjusted based on moisture of 15.5% 10 .…”

Impact of IDH Mutations on DNA Methylation of Acute Myeloid Leukemia Related Genes: A Review Article

“…Acute myeloid leukaemia (AML) is a heterogeneous disease defined by the unregulated growth of proliferative progenitor cells that are incapable of terminal differentiation. It is now obvious that numerous genes are often altered in AML (1). However, it is really challenging to identify transformation driver genes within this large number of leukaemia associated disrupted genes.…”