Expressions of Interleukin-1β and Interleukin-6 Within Aortas and Uteri of Rats with Various Severities of Ligature-Induced Periodontitis

Abstract

To investigate the associations of periodontitis with histological lesions in some other organs, various severities of periodontitis were induced in rats by 3/0 silk ligatures tied around different numbers of their molar necks. Six weeks after the initial placement of ligatures, all rats were sacrificed by an anaesthetic overdose. The distances from the cemento-enamel junction to the alveolar bone crest within the placement zone of the ligature and their contralateral zone in groups L(2) and L(3) were measured. The levels of interleukin (IL)-1β and IL-6 in serum were assayed by enzyme-linked immunosorbent assay techniques, and those within aortas and uteri were measured by real-time polymerase chain reaction and by immunohistochemistry. We divided the ligature-induced periodontitis models into mild, moderate and severe rat periodontitis and observed that although no association between periodontitis and the serum concentrations of IL-1β was detected, the differences in the severity of rat periodontitis led to varying degrees of elevated expressions of IL-1β and IL-6 within aortas and uteri.

IL-6 Induced by Periodontal Inflammation Causes Neuroinflammation and Disrupts the Blood–Brain Barrier

“…Ma et al reported that ligature increased the serum IL-6 levels but not those of IL-1β. consistent with our findings [ 42 ]. This suggests that the serum cytokine levels in ligature-induced PD is not reflected in periodontal tissue inflammation.…”

Endothelial dysfunction in rats with ligature-induced periodontitis: Participation of nitric oxide and cycloxygenase-2-derived products

“…the systemic vasculature, as this bacteria has been found in atheroma plaques (Haraszthy et al, 2000) and thrombi from patients with acute myocardial infarction (Ohki et al, 2012). In addition, and strengthening the validity of the animal model used in this study, increased nitration of cardiac proteins (Ma, Guo, You, Xia, & Yan, 2011) and enhanced lipid deposits in aorta (Bain et al, 2009) have been detected in rats seven days after ligature induced-periodontitis.…”

Nonsurgical periodontal treatment reduced aortic inflammation in ApoE−/− mice with periodontitis

“…In addition, accelerated atherosclerosis progress has been found in apoE −/− mice by oral or intravenous inoculation of P. gingivalis or Fusobacterium nulceatum (Lalla et al 2003 ; Li et al 2002 ; Velsko et al 2015 ). Furthermore, a ligature-induced periodontitis rat model, increased lipid peroxidation and IL-6 expression was observed in aortic wall (Ma et al 2010 ). In the present study, decreased periodontal destruction after NSPT was observed, therefore, less periodontal pathogens and their byproducts was released into the blood stream, leading to less inflammatory response in the arterial wall.…”