Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development

Abstract

SRY-related box (Sox) transcription factors are conserved among vertebrate species. These proteins regulate multiple processes including sex determination and testis differentiation of the male embryo. Although members of the Sox family have been identified in pre- and postnatal Sertoli cells, they have never been characterized in adult Leydig cells. The objectives of this research were to identify expressions of Sox9, Sox5, and Sox13 in mice Leydig cell cultures and to establish their expression profiles in postnatal mice testes at different developmental stages. Methods used include Western blots and qPCR of stimulated MA-10 cell cultures and whole mice testes. Sox9, Sox5, and Sox13 proteins were detected in MA-10 cells as well as whole mouse testis. Although Sox9, Sox5, and Sox13 mRNA levels from whole mice testes tended to increase according to postnatal development, these results were not significant. Sox members were also detected in whole mice testis by Western Blot. However, Sox9, Sox5, and Sox13 protein expressions remained relatively constant during postnatal development from postnatal (P) day 60 to P365. Being newly characterized in the mouse testis, Sox13 was mainly localized by immunofluorescence within the nuclei of cells from seminiferous tubules, possibly spermatocytes and Sertoli cells. In addition, Sox9, Sox5, and Sox13 proteins were characterized in the nuclei of MA-10 Leydig cell cultures. Their expressions and transcriptional activities remained unaffected by activators of the cAMP/PKA pathway. Thus, Sox9, Sox5, and Sox13 transcription factors are expressed in postnatal testis and may regulate multiple functions such as steroidogenesis and spermatogenesis.

Gelişme Dönemindeki Fare Testislerinde Sertoli Hücreleri ve Spermatogonial Hücrelerde Sox5 ve Sox6 Ekspresyonu

“…Their expression exists in Sertoli cells but fainter than spermatogonial cells. These results are consistent with the previous researches [12,14,16] . It means that these genes at adult age are more functional and assists in sperm maturation.…”

“…The expression of SoxD family genes are reported in various tissues and cells in testis, neuron, oligodendrocytes, chondrocytes and palatogenesis [16,32,33] . A member of this family (Sox5) has been reported in the postnatal period in testes [14] , which concede with our findings in spermatogonial cells. The S-Sox5, a short transcript, in human and mouse having 48kD size was also found in testes in ciliated/flagellated cells; the short isoform of Sox5 is deficient in N-terminus and have a length of half of the long transcript (6kb).…”

“…Sox6 is expressed in full length but Sox5 appears in short and long form in testis. Sox5 gene was reported earlier and later on its isoforms were also identified [13,14] . These genes are involved in different pathways including chondrogenesis and development of nervous system [13][14][15] .…”

Characterization of rodent Sertoli cell primary cultures

“…Besides, its expression was also described in Leydig cells (Daigle, Roumaud, & Martin, 2015). blasts (Stilley et al, 2014).…”

Construction and expression of a prokaryotic expression vector for the goat sry gene

“…Sex selection is very significant for the breeding and production of livestock and the prevention and treatment of genetic diseases (Han et al, 2008). The five most important genes for the determination of embryonic sex (SRY, SOX9, WT-1, SF-1 and DAX-1) have been identified, and among them, SRY is the most interesting gene (Daigle et al, 2015;Kozhukhar ', 2012;Tanaka et al, 2014;Mohamed, 2013;Suntharalingham et al, 2015). The SRY gene is the initial autonomous regulatory factor of testicular development, playing a central role in the process (Larney et al, 2014;Waters et al, 2007).…”