Expulsion of Nematospiroides dubius from the intestine of mice treated with immune serum

Behnke, Jerzy M.; Parish, Heather A.

doi:10.1111/j.1365-3024.1979.tb00692.x

Cited by 59 publications

(36 citation statements)

References 23 publications

(15 reference statements)

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“…Furthermore, it has been demonstrated that transfer of immune serum could confer resistance to Hp, Nb, and Ascaris suum (27)(28)(29)(30). However, how antibodies execute their protective effect remains unclear.…”

Section: Significancementioning

confidence: 99%

Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion

Schwartz

Turqueti-Neves

Hartmann

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Gastrointestinal worms (helminths) infect more than 2 billion people, and vaccines are not yet available. Helminths elicit a type 2 immune response characterized by high serum IgE levels and increased numbers of IL-4– or IL-13–secreting effector cells including Th2 cells, eosinophils, basophils, and type 2 innate lymphoid cells. We determined the mechanism by which basophils contribute to protection against secondary infections with gastrointestinal helminths. Here we demonstrate that basophils are recruited into the small intestine of infected mice and orchestrate the local type 2 immune response in this tissue. Basophil-mediated protection required the presence of IgE and the expression of activating Fc receptors and IL-4/IL-13 in basophils. These findings could help the development of new vaccination strategies against helminths.

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Section: Significancementioning

confidence: 99%

Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion

Schwartz

Turqueti-Neves

Hartmann

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Mouse samples were assayed individually; serum was tested at a dilution of 1:50 and intestinal samples were used undiluted. Each plate included control 'hyper-immune' serum (HIS), (Behnke & Parish, 1979) and also serum from naive mice (NS). With regard to IgE levels, the optical density (OD) for each sample minus the OD of the NS was expressed as a percentage of the OD of the HIS minus the NS, giving a relative response index (RRI).…”

Section: Measurement Of H Polygyrus-specific Igg2a and Ige Responsesmentioning

confidence: 99%

The relative involvement of Th1 and Th2 associated immune responses in the expulsion of a primary infection of Heligmosomoides polygyrus in mice of differing response phenotype

2003

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T helper cell (Th1 and Th2) associated responses were examined following a primary infection with the gastrointestinal nematode Heligmosomoides polygyrus in five inbred strains of mice with different resistance phenotypes. Levels of (i) mast cell protease, (ii) specific IgE, (iii) nitric oxide and (iv) specific IgG2a, as markers of Th2 and Th1 associated responses, respectively, were determined in sera and intestinal fluids and correlated with worm burdens. The 'fast' responder (resistant) strains SWR and SJL produced strong Th2 and Th1 associated responses respectively in a mutually exclusive fashion. The F 1 hybrid (SWR £ SJL) F 1 , showed rapid expulsion of the parasite and expressed both intense Th1 and Th2 responses, suggesting synergism between Th1 and Th2 activity in these mice. The results indicate that both Th2 and Th1 responses operate in mice following a primary infection with H. polygyrus and that each Th response may be involved to a greater or lesser degree within certain strains. Resistance to H. polygyrus was found to correlate only to the intensity of either the gut-associated mastocytosis or nitric oxide production in these strains but not to either specific IgE or IgG2a titres. Chronic infections in the 'slow' response phenotype mouse strains CBA and C57BL/10, were associated with both poor Th2 and poor Th1-associated responses attributed to a general parasite-mediated immunosuppression of the host immune response to infection.

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“…Sera were assayed individually in triplicate after storage at -40°C and a mean optical density (OD) value was obtained for each serum. Each plate included, control hyperimmune serum (HIM) which was raised as described by Behnke & Parish (1979) and control serum from naive mice and these were used to calculate the RRT for individual sera using thc following formula: kits purchased from The Binding Site, Birmingham. Ring diameters were measured in two directions at 90" and the mean was used to calculate the concentration of immunoglobulins from a calibration curve obtained using appropriate standards.…”

Section: Measurement Of Antibody Responsesmentioning

confidence: 99%

Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): parasite specific IgG1 antibody responses and primary response phenotype

Wahid

Behnke

1993

Parasite Immunology

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Expulsion of Nematospiroides dubius from the intestine of mice treated with immune serum

Cited by 59 publications

References 23 publications

Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion

Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion

The relative involvement of Th1 and Th2 associated immune responses in the expulsion of a primary infection of Heligmosomoides polygyrus in mice of differing response phenotype

Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): parasite specific IgG1 antibody responses and primary response phenotype

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