Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones

Kronvall, Göran; Larsson, Mattias; Borén, Charlotte; Kahlmeter, Gunnar; Bartoloni, Alessandro; Rossolini, Gian María; Grape, Malin; Kristiansson, Charlotte; Karlsson, Inga

doi:10.1016/j.ijantimicag.2005.09.005

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…However, due to the low sensitivity of the detection method of resistant E. coli (using non-selective media for isolation), the presence of resistant E. coli may be more frequent in pork than indicated by this study. 19 The main indication for apramycin is treatment of E. coli diarrhoea in piglet nurseries and post-weaning medication. [20][21][22][23] In Denmark, apramycin was used in 1% (54 ADD 15 /1000 pigs) of the antimicrobial treatments of diarrhoea in weaning pigs in 2004 (total gastrointestinal treatment was 78% of 7100 ADD 15 / 1000 pigs 13 ), but the percentage is probably higher for the main indication, E. coli diarrhoea.…”

Section: Discussionmentioning

confidence: 99%

Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli

Jensen¹

2006

Journal of Antimicrobial Chemotherapy

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Section: Discussionmentioning

confidence: 99%

Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli

Jensen¹

2006

Journal of Antimicrobial Chemotherapy

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“…Screening for antibiotic-resistant E. coli in the faecal microbiota was carried out by a direct plating method, as described previously [18][19][20]. Briefly, each swab was spread on a MacConkey Agar No.…”

Section: Screening For Resistant E Coli In the Commensal Microbiotamentioning

confidence: 99%

Antibiotic resistance in a very remote Amazonas community

Bartoloni¹,

Pallecchi

Rodríguez³

et al. 2009

International Journal of Antimicrobial Agents

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“…Several different approaches to the collection and analysis of isolates have been taken in previous studies of antibiotic resistance in commensal E. coli. Some have recovered the less abundant resistant populations by selecting for them directly (Guinée et al, 1970;Lester et al, 1990;Kronvall et al, 2005;Skurnik et al, 2008), but most have examined between one and five E. coli colonies per faecal sample (Linton et al, 1972;London et al, 1993;Osterblad et al, 2000). The latter approach favours selection of the dominant clone (Lidin-Janson et al, 1978) and does not allow reliable detection of resistance in minority members of the E. coli community, even though these can play an important role in the dissemination of resistance determinants.…”

Section: Introductionmentioning

confidence: 99%

Commensal Escherichia coli of healthy humans: a reservoir for antibiotic-resistance determinants

Bailey

Pinyon

Anantham

et al. 2010

Journal of Medical Microbiology

191

135

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This study examined in detail the population structure of Escherichia coli from healthy adults with respect to the prevalence of antibiotic resistance and specific resistance determinants. E. coli isolated from the faeces of 20 healthy adults not recently exposed to antibiotics was tested for resistance to ten antibiotics and for carriage of integrons and resistance determinants using PCR. Strain diversity was assessed using biochemical and molecular criteria. E. coli was present in 19 subjects at levels ranging from 2.0¾10 4 to 1.7¾10 8 c.f.u. (g faeces). Strains resistant to one to six antibiotics were found at high levels (.30 %) in only ten individuals, but at significant levels (.0.5 %) in 14. Resistant isolates with the same phenotype from the same individual were indistinguishable, but more than one susceptible strain was sometimes found. Overall, individuals harboured one to four E. coli strains, although in 17 samples one strain was dominant (.70 % of isolates). Eighteen strains resistant to ampicillin, sulfamethoxazole, tetracycline and trimethoprim in 15 different combinations were observed. One resistant strain was carried by two unrelated individuals and a susceptible strain was shared by two cohabiting subjects. Two minority strains were derivatives of a more abundant resistant strain in the same sample, showing that continuous evolution is occurring in vivo. The trimethoprim-resistance genes dfrA1, dfrA5, dfrA7, dfrA12 or dfrA17 were in cassettes in a class 1 or class 2 integron. Ampicillin resistance was conferred by the bla TEM gene, sulfamethoxazole resistance by sul1, sul2 or sul3 and tetracycline resistance by tetA(A) or tetA(B). Chloramphenicol resistance (cmlA1 gene) was detected only once. Phylogenetic groups A and B2 were more common than B1 and D. Commensal E. coli of healthy humans represent an important reservoir for numerous antibiotic-resistance genes in many combinations. However, measuring the true extent of resistance carriage in commensal E. coli requires in-depth analysis.

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Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones

Cited by 7 publications

References 27 publications

Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli

Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli

Antibiotic resistance in a very remote Amazonas community

Commensal Escherichia coli of healthy humans: a reservoir for antibiotic-resistance determinants

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