Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in SITU Hybridization

Shi, Shan‐Rong; Taylor, Clive R.

doi:10.1002/9780470875612.ch2

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…Second, as a direct result of this extended capability, the value of archival FFPE tissue blocks, accompanied by known follow-up data, was enormously enhanced, providing a valuable resource for translational clinical research and basic research that cannot easily be reproduced. Third, AR is a simple, inexpensive, and effective technique that leads to satisfactory IHC staining results in FFPE tissue for a great number of antibodies tested (Shi et al 1995;Shi et al 1997;Taylor and Cote 2005;Yamashita 2007;Shi and Taylor 2010a;Taylor et al 2010). Finally, and by no means least, there is a growing body of recent literature with respect to use of AR methods for extraction of molecules (DNA, RNA, and proteins) from FFPE tissues that has further extended the utility of archival tissues and promises to allow the combination of proteomics and IHC in a true molecular morphology approach.…”

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confidence: 99%

Antigen Retrieval Immunohistochemistry

Shi

Taylor

2011

J Histochem Cytochem.

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235

105

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SummaryAs a review for the 20th anniversary of publishing the antigen retrieval (AR) technique in this journal, the authors intend briefly to summarize developments in AR-immunohistochemistry (IHC)-based research and diagnostics, with particular emphasis on current challenges and future research directions. Over the past 20 years, the efforts of many different investigators have coalesced in extending the AR approach to all areas of anatomic pathology diagnosis and research and further have led to AR-based protein extraction techniques and tissue-based proteomics. As a result, formalin-fixed paraffinembedded (FFPE) archival tissue collections are now seen as a literal treasure of materials for clinical and translational research to an extent unimaginable just two decades ago. Further research in AR-IHC is likely to focus on tissue proteomics, developing a more efficient protocol for protein extraction from FFPE tissue based on the AR principle, and combining the proteomics approach with AR-IHC to establish a practical, sophisticated platform for identifying and using biomarkers in

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confidence: 99%

Antigen Retrieval Immunohistochemistry

Shi

Taylor

2011

J Histochem Cytochem.

Self Cite

235

105

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“…After this initial paper, a considerable number of authors have been using the technique in different forms, i.e., heat sources (e.g., microwave , hot plate ) and “in solution” (e.g., aluminum chloride , citric acid ). It has been demonstrated that AR, which is simple and inexpensive, leads to adequate immunohistochemistry staining results in formalin-fixed tissue for a great number of antibodies tested ( Yamashita, 2007 ; D’Amico et al, 2009 ; Shi and Taylor, 2010 ). As a result of all these efforts, existing formalin-fixed archival tissue collections are gaining new value.…”

Section: Methodsmentioning

confidence: 99%

Histological Characterization of the Irritative Zones in Focal Cortical Dysplasia Using a Preclinical Rat Model

Deshmukh

Leichner

Bae

et al. 2018

Front. Cell. Neurosci.

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Current clinical practice in focal epilepsy involves brain source imaging (BSI) to localize brain areas where from interictal epileptiform discharges (IEDs) emerge. These areas, named irritative zones, have been useful to define candidate seizures-onset zones during pre-surgical workup. Since human histological data are mostly available from final resected zones, systematic studies characterizing pathophysiological mechanisms and abnormal molecular/cellular substrates in irritative zones—independent of them being epileptogenic—are challenging. Combining BSI and histological analysis from all types of irritative zones is only possible through the use of preclinical animal models. Here, we recorded 32-channel spontaneous electroencephalographic data from rats that have focal cortical dysplasia (FCD) and chronic seizures. BSI for different IED subtypes was performed using the methodology presented in Bae et al. (2015). Post-mortem brain sections containing irritative zones were stained to quantify anatomical, functional, and inflammatory biomarkers specific for epileptogenesis, and the results were compared with those obtained using the contralateral healthy brain tissue. We found abnormal anatomical structures in all irritative zones (i.e., larger neuronal processes, glioreactivity, and vascular cuffing) and larger expressions for neurotransmission (i.e., NR2B) and inflammation (i.e., ILβ1, TNFα and HMGB1). We conclude that irritative zones in this rat preclinical model of FCD comprise abnormal tissues disregarding whether they are actually involved in icto-genesis or not. We hypothesize that seizure perpetuation happens gradually; hence, our results could support the use of IED-based BSI for the early diagnosis and preventive treatment of potential epileptic foci. Further verifications in humans are yet needed.

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“…If this step is not performed, the antibodies will not have complete access to the tissue and will be unable to bind to the correct epitope. Antigen retrieval leads to satisfactory immunohistochemical staining in paraffin-embedded tissue for a great number of antibodies tested [ 16 , 17 , 18 ]. An apparent difference in the condition of immunohistochemical staining was ascertained between GLP-1 and GLP-2.…”

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confidence: 99%

Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry

Monir

Hiramatsu

Nishimura

et al. 2014

The Journal of Veterinary Medical Science

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An antigen retrieval method for immunohistochemical staining of glucagon-like peptide (GLP)-2-immunoreactive cells was investigated in the chicken small intestine. GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological features of GLP-2-immunoreactive cells between treated and untreated preparations. The frequencies of occurrence of GLP-2-immunoreactive cells, however, were significantly different in treated and untreated preparations: in the proximal and distal regions of jejunum and ileum obtained from untreated preparations, the frequencies of occurrence were 0.5 ± 0.2, 0.7 ± 0.1, 0.9 ± 0.2 and 1.5 ± 0.3, respectively (cell numbers per mucosal area: cells/mm2, mean ± SD), whereas those from treated sections were 14.7 ± 2.3, 19.8 ± 2.3, 23.5 ± 4.7 and 34.6 ± 4.9 cells/mm2, respectively. These data indicate that this antigen retrieval method is able to make immunoreactive GLP-2 available for detection and that GLP-2 may act as one of the common hormones secreted by L cells in the chicken small intestine.

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Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in SITU Hybridization

Cited by 4 publications

References 66 publications

Antigen Retrieval Immunohistochemistry

Antigen Retrieval Immunohistochemistry

Histological Characterization of the Irritative Zones in Focal Cortical Dysplasia Using a Preclinical Rat Model

Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry

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