Extended depth of field through an axicon

Zhai, Zhongsheng; Ding, Shuai; Lv, Qinghua; Wang, Xuanze; Zhong, Yanfei

doi:10.1080/09500340903082689

Cited by 47 publications

(24 citation statements)

References 17 publications

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“…The traditional image restoration method is working with degradation function to recover the blurred image. However, the results of image restored are not satisfactory because it is difficult to get an accurate degradation function and they exhibit poor contrast [13].…”

Section: Introductionmentioning

confidence: 98%

“…Chromatic aberration can produce axial displacement of the focal segment; some researchers seek to obtain an achromatic zone where all colors overlap [12]. In an early publication [13], we have provided an approach to extend the depth of field through an axicon. Though the intermediate images received by the detector are insensitive to defocus a large depth of field range, they are not clear.…”

Section: Introductionmentioning

confidence: 98%

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A non-diffracting image restoration based on multiple images fusion

Zhai

Zhang

et al. 2013

Optics Communications

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

A non-diffracting image restoration based on multiple images fusion

Zhai

Zhang

et al. 2013

Optics Communications

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“…In contrast, the CPM-PSF with a moderate EDF becomes aberrated at this sample depth. Although EDFM can be achieved through other methods that are either purely optical [16][17][18] or purely digital, 19,20 the wide-field wavefront encoding approach has the advantage of simultaneously fast acquisition and high resolution with low intensity exposure that is particularly important in live-cell fluorescence microscopy. Although wavefront encoding is an established method to achieve EDFM, 12 the issue of depth variability due to SA in high-NA microscopy imaging was not explicitly addressed.…”

Section: Introductionmentioning

confidence: 99%

Performance evaluation of extended depth of field microscopy in the presence of spherical aberration and noise

2018

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Effectiveness of extended depth of field microscopy (EDFM) implementation with wavefront encoding methods is reduced by depth-induced spherical aberration (SA) due to reliance of this approach on a defined point spread function (PSF). Evaluation of the engineered PSF's robustness to SA, when a specific phase mask design is used, is presented in terms of the final restored image quality. Synthetic intermediate images were generated using selected generalized cubic and cubic phase mask designs. Experimental intermediate images were acquired using the same phase mask designs projected from a liquid crystal spatial light modulator. Intermediate images were restored using the penalized space-invariant expectation maximization and the regularized linear least squares algorithms. In the presence of depth-induced SA, systems characterized by radially symmetric PSFs, coupled with model-based computational methods, achieve microscope imaging performance with fewer deviations in structural fidelity (e.g., artifacts) in simulation and experiment and 50% more accurate positioning of 1-μm beads at 10-μm depth in simulation than those with radially asymmetric PSFs. Despite a drop in the signal-to-noise ratio after processing, EDFM is shown to achieve the conventional resolution limit when a model-based reconstruction algorithm with appropriate regularization is used. These trends are also found in images of fixed fluorescently labeled brine shrimp, not adjacent to the coverslip, and fluorescently labeled mitochondria in live cells.

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“…Another interesting feature of axicon lens is its extended focal depth and this feature has been used in many studies [17][18][19][20]. Though axicon lens has these interesting features, they have not been relatively explored to its potential in the imaging context of viewing behind opaque obstacles.…”

Section: Introductionmentioning

confidence: 99%

Imaging behind opaque obstacle: a potential method for guided in vitro needle placement

Perinchery

Shinde

Murukeshan

2016

Biomed. Opt. Express

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Abstract:We report a simple real time optical imaging concept using an axicon lens to image the object kept behind opaque obstacles in free space. The proposed concept underlines the importance and advantages of using an axicon lens compared to a conventional lens to image behind the obstacle. The potential of this imaging concept is demonstrated by imaging the insertion of surgical needle in biological specimen in real time, without blocking the field of view. It is envisaged that this proposed concepts and methodology can make a telling impact in a wide variety of areas especially for diagnostics, therapeutics and microscopy applications.

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Extended depth of field through an axicon

Cited by 47 publications

References 17 publications

A non-diffracting image restoration based on multiple images fusion

A non-diffracting image restoration based on multiple images fusion

Performance evaluation of extended depth of field microscopy in the presence of spherical aberration and noise

Imaging behind opaque obstacle: a potential method for guided in vitro needle placement

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