Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics

Muller, Leslie; Fornecker, Luc Mathieu; Chion, Marie; Dorsselaer, Alain Van; Cianférani, Sarah; Rabilloud, Thierry; Carapito, Christine

doi:10.1038/s41598-018-26600-4

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…For the shotgun proteomic analysis, the samples were included in polyacrylamide plugs according to Muller et al, with some modifications to downscale the process [32]. To this purpose, the photopolymerization system using methylene blue, toluene sulfinate and diphenyliodonium chloride was used [33].…”

Section: Shotgun Approachmentioning

confidence: 99%

Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis

Cavazza

Collin-Faure

Pérard

et al. 2022

Journal of Proteomics

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Section: Shotgun Approachmentioning

confidence: 99%

Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis

Cavazza

Collin-Faure

Pérard

et al. 2022

Journal of Proteomics

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“…Shotgun proteomics. For the shotgun proteomic analysis, the samples were included in polyacrylamide plugs according to Muller et al 51 with some modifications to downscale the process. 52 To this purpose, the photopolymerization system using methylene blue, toluene sulfinate and diphenyliodonium chloride was used.…”

Section: Surface Marker Assaysmentioning

confidence: 99%

Does size matter? A proteomics-informed comparison of the effects of polystyrene beads of different sizes on macrophages

Collin-Faure

Dalzon

Devcic

et al. 2022

Environ. Sci.: Nano

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“…For the shotgun proteomic analysis, the samples were included in polyacrylamide plugs according to Muller et al [43], with some modifications to downscale the process [44]. To this purpose, the photopolymerization system using methylene blue, toluene sulfonate, and diphenyliodonium chloride was used [45].…”

Section: Sample Processing and Mass Spectrometrymentioning

confidence: 99%

Repeated Exposure of Macrophages to Synthetic Amorphous Silica Induces Adaptive Proteome Changes and a Moderate Cell Activation

et al. 2022

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Synthetic amorphous silica (SAS) is a nanomaterial used in a wide variety of applications, including the use as a food additive. Two types of SAS are commonly employed as a powder additive, precipitated silica and fumed silica. Numerous studies have investigated the effects of synthetic amorphous silica on mammalian cells. However, most of them have used an exposure scheme based on a single dose of SAS. In this study, we have used instead a repeated 10-day exposure scheme in an effort to better simulate the occupational exposure encountered in daily life by consumers and workers. As a biological model, we have used the murine macrophage cell line J774A.1, as macrophages are very important innate immune cells in the response to particulate materials. In order to obtain a better appraisal of the macrophage responses to this repeated exposure to SAS, we have used proteomics as a wide-scale approach. Furthermore, some of the biological pathways detected as modulated by the exposure to SAS by the proteomic experiments have been validated through targeted experiments. Overall, proteomics showed that precipitated SAS induced a more important macrophage response than fumed SAS at equal dose. Nevertheless, validation experiments showed that most of the responses detected by proteomics are indeed adaptive, as the cellular homeostasis appeared to be maintained at the end of the exposure. For example, the intracellular glutathione levels or the mitochondrial transmembrane potential at the end of the 10 days exposure were similar for SAS-exposed cells and for unexposed cells. Similarly, no gross lysosomal damage was observed after repeated exposure to SAS. Nevertheless, important functions of macrophages such as phagocytosis, TNFα, and interleukin-6 secretion were up-modulated after exposure, as was the expression of important membrane proteins such as the scavenger receptors, MHC-II, or the MAC-1 receptor. These results suggest that repeated exposure to low doses of SAS slightly modulates the immune functions of macrophages, which may alter the homeostasis of the immune system.

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Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics

Cited by 8 publications

References 27 publications

Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis

Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis

Does size matter? A proteomics-informed comparison of the effects of polystyrene beads of different sizes on macrophages

Repeated Exposure of Macrophages to Synthetic Amorphous Silica Induces Adaptive Proteome Changes and a Moderate Cell Activation

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