Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolated from Egyptian Patients with Suspected Blood Stream Infection

Abdallah, Hossam M.; Wintermans, B.B.; Reuland, E.A.; Koek, Alex; Naiemi, Nashwan al; Ammar, Ahmed M.; Mohamed, Amany Abdel-Rahman; Vandenbroucke-Grauls, Christina M. J. E.

doi:10.1371/journal.pone.0128120

Cited by 44 publications

(46 citation statements)

References 38 publications

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“…In our study, the frequency of BSIs by Enterobacteriaceae was found to be 1.7% (192/11,264) out of total blood culture positive 17.3% (1948/11,264). A similar study conducted by Abdallah et al in 2015 in Egyptian patients and in Nepal by Joshy M Easow et al in 2010 where the number of Enterobacteriaceae isolated found to be 94 and 96 respectively, which was less as compared to our study [17,18].…”

Section: Discussioncontrasting

confidence: 54%

“…Particularly resistance in GNB is of great importance as there is a dearth of novel antibiotics directed against these organisms [16]. Among the antimicrobials used, β-lactams are the most commonly used therapeutic class of antimicrobials for treatment of bacterial infections because of their broad antibacterial spectrum and excellent safety pro le [17].…”

Section: Discussionmentioning

confidence: 99%

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Multidrug resistant Enterobacteriaceae from blood stream infections at a tertiary care hospital of Eastern Nepal

Yadav¹,

Bhattarai²,

Khanal³

2020

Preprint

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Background Blood stream infection (BSI) is one of the major causes of morbidity and mortality worldwide. It has poses significant challenge to the clinicians and clinical microbiologists alike. Therefore its accurate diagnosis, isolation and identification of causative agents with appropriate antibiotics is required. This study is aimed to find out resistance pattern, e.g. extended-spectrum-beta-lactamase (ESBL), AmpC, K1, carbapenemase and metallo-β-lactamase (MBL) among isolates obtained from BSI. Methods A cross-sectional study was conducted in the Department of Microbiology, BPKIHS from 1st September 2014 to 31st August 2015. Isolates were screened for ESBL, AmpC, K1, and carbapenemase production by ten disk method. Confirmation for ESBL was done phenotypically by using combined disk method recommended by CLSI, AmpC sterile disk method for AmpC and K1 by combined disk method. Metallo-beta-lactamase (MBL) production was detected by imipenem-ethylene-diamine-tetra acetic acid double disk synergy test. Results A total of 11,264 blood samples were collected from the patients suspected of Blood Stream Infection. Of these isolates, 192 (1.70%) were Enterobacteriaceae. Among them, 94 (49%) were ESBL, 51 (26.5%) were carbapenemase and 10 (5%) were AmpC producers. Of 51 carbapenemase producers, 22 (11.5%) were MBL producers. None of the isolates were found to produce K1 β-lactamase. A total of 64 (33.4%) isolates were MDR. Conclusion MDR Enterobacteriaceae is found to be prevalent in our set up as important cause of BSI.

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Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Multidrug resistant Enterobacteriaceae from blood stream infections at a tertiary care hospital of Eastern Nepal

Yadav¹,

Bhattarai²,

Khanal³

2020

Preprint

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“…Resistance of pathogenic bacteria to β-lactam antibiotics, a group of antibiotic mostly used for the treatment of bacterial infections because of their broad antibacterial spectrum and excellent safety profile has taken a great threatening dimension with the emergence Extended-Spectrum Β-Lactamase (ESBL) producing Enterobacteriaceae (Abdallah et al, 2015). The ESBLs first described in 1983 in Germany arose from a single nucleotide polymorphism in the bla SHV genes that altered specificity to oxyimino-cephalosporins.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and resistance profile of extended-spectrum -lactamases-producing Enterobacteriaceae in Ouagadougou, Burkina Faso

Kpoda¹,

Nathalie²,

Bonkoungou³

et al. 2017

Afr. J. Microbiol. Res.

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Increasing bacterial resistance to antimicrobial agents has become an issue of concern. A major problem of the treatment of infections caused by Enterobacteriaceae using antibiotics is the emergence of Extended-spectrum β-lactamases (ESBL)-producing Enterobacteriaceae. This study aims to determine the prevalence of ESBL-producing Enterobacteriaceae strains in Ouagadougou, Burkina Faso, and describe their resistance profile to other antibiotics commonly used in the infections treatment. 486 clinical strains of Enterobacteriaceae were obtained from patients attending three health centers in Ouagadougou (Burkina Faso) from November 2014 to October 2015. Biochemical identification was performed and antibiotics susceptibility test was performed using the disk diffusion method. Data was analyzed with the Excel and ANOVA one-way software GraphPad Prism version 5.01 software. Results revealed occurrence of Escherichia coli (60.9%, 194) predominated followed by Klebsiella spp. (22.4%, 109). Antibiotics susceptibility test revealed that 86.8% strains were resistant to amoxicillin, 81.3% to trimethoprim-sulfamethoxazole, 61.9% to ceftriaxone, 58.6% to cefotaxime and 58.4% to cefepime. It was observed that 99.8% were susceptible to imipenem while 16.6% were resistant to fosfomycin and 12.3% to amikacin. However, 38.5% (187/486) of the strains were ESBL-producing, 67.9% (127/187) of which came from Yalgado Ouedraogo University Hospital Center, 23.5% (44/187) from Charles De Gaulle Paediatric University Hospital Center and 8.6% (16/187) from Saint Camille Hospital. This study thus showed a high prevalence of Extended-Spectrum Β-Lactamases producing Enterobacteriaceae strains in Ouagadougou (38.5%). It underlined the need for routine detection and systematic reporting of ESBL strains in different health facilities in Burkina Faso, so that measures could be taken to prevent their spread and treatment failures.

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“…12,[45][46] The coexistence of multiple ESBL genes within individual isolates is of concern because it has been reported that such isolates had a greater resistance to various classes of antibiotics mediated through acquisition of genes located on mobile genetic elements. 47 In our study, all ESBL-producing isolates showed a low level of susceptibility to most antimicrobial agents tested and this could be explained by the fact that the ESBL producers usually carry a multiresistant plasmid, and these also carry genes conferring resistance to b-lactam and non-blactam antibiotics.…”

Section: Discussionmentioning

confidence: 99%

First Report of Extended-Spectrum β-Lactamases Among Clinical Isolates ofKlebsiella pneumoniaein Gaza Strip, Palestine

Tayh

SallemRym

YahiaHoussem

et al. 2017

Microbial Drug Resistance

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Sixteen broad-spectrum cephalosporin-resistant Klebsiella pneumoniae isolates were recovered between April and June 2013 from Palestinian hospitals, Gaza Strip. Genes encoding extended-spectrum beta-lactamases (ESBLs) and other resistance genes were characterized by polymerase chain reaction and sequencing. The following β-lactamase genes were detected: bla+ bla+ bla (six isolates), bla+ bla+ bla (two isolates), bla (two isolates), bla+ bla (one isolate), bla+ bla (one isolate), bla+ bla+ bla(one isolate), bla+ bla (one isolate), bla+ bla (one isolate), and bla (one isolate). The ISEcp1 (in four cases truncated by IS26), orf477, or IS903 sequences were found upstream or downstream of bla genes. The aac(6')-Ib-cr gene was found in seven isolates. The qnrS1 and qnrB1 genes were detected in five isolates and two isolates, respectively. Seven isolates contained class 1 integrons with four gene cassette arrangements: dfrA5 (three isolates), dfrA12-orf-aadA2 (two isolates), dfrA17-aadA5 (one isolate), and aadA1 (one isolate). A high clonal diversity was also observed among studied isolates by pulsed-field gel electrophoresis (12 unrelated profiles). This study demonstrates for the first time the emergence and polyclonal spread of multidrug-resistant ESBL-producing K. pneumoniae isolates among patients in a hospital setting in Gaza Strip, Palestine.

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Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolated from Egyptian Patients with Suspected Blood Stream Infection

Cited by 44 publications

References 38 publications

Multidrug resistant Enterobacteriaceae from blood stream infections at a tertiary care hospital of Eastern Nepal

Multidrug resistant Enterobacteriaceae from blood stream infections at a tertiary care hospital of Eastern Nepal

Prevalence and resistance profile of extended-spectrum -lactamases-producing Enterobacteriaceae in Ouagadougou, Burkina Faso

First Report of Extended-Spectrum β-Lactamases Among Clinical Isolates ofKlebsiella pneumoniaein Gaza Strip, Palestine

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