Extended-Spectrum β-Lactamase–Producing<i>Enterobacteriaceae</i>in a Malian Orphanage

Tandé, Didier; Jallot, Nelle; Bougoudogo, F; Montagnon, Tracey; Gouriou, S.; Sizun, Jacques

doi:10.3201/eid1503.071637

Cited by 36 publications

(20 citation statements)

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“…ESBLs are not just nosocomial infections in big hospitals in Ghana, but are spreading into the communities as well (Feglo et al, 2013). In this present study 43/51(84%) of the urinary E. coli isolates produced ESBL and 16(37.2%) were community isolates, an observation, which confirms findings in other studies that ESBL-producing Enterobacteria are detectable in both community and hospitalized patients with varying prevalence levels as 63% was reported from Mali (Tande et al, 2009), 43% in Ghana (Feglo et al, 2013), 40% in Niger (Woerther et al, 2011) and 26% in Kenya (Kariuki et al, 2007) and 28.66% in Bangladesh (Islam et al, 2017). It was estimated that ESBL prevalence of 80% in a hospital setting will lead to as much as 80% of treatments failures and 35% Bla CTX-M bands (593pbs) mortality (Perez et al, 2007).…”

Section: Discussionsupporting

confidence: 91%

Molecular Characterization of Extended-Spectrum Beta-Lactamase Producing Urinary Escherichia coli Isolated in Brong-Ahafo Regional Hospital, Ghana

Wireko

Frimpong

Feglo

2017

ESJ

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Introduction. Antimicrobial resistance is a growing international problem resulting from the enzyme β-lactamase production by bacteria, to degrade antibiotics, especially β-lactam antibiotics. In Brong Ahafo Regional Hospital, Sunyani, these antibiotics are heavily depended upon for the treatment of serious infections, but unfortunately high proportions of bacterial isolates in the hospital, have been found to be resistant to the commonly prescribed antibiotics. This study aims to determine the prevalence of ESBL-producing E. coli so as to determine if ESBL are responsible for the high antimicrobial resistance seen at the Brong-Ahafo Regional Hospital, Sunyani. Methods. The study was a cross sectional study, involving 51 E. coli isolates from urine samples of both in-and out-patients between January and December, 2014. Antimicrobial susceptibility profiles of the isolates were determined by the Kirby Bauer disc diffusion method against 12 antibiotics. The isolates were screened for ESBL production, and then confirmed by the combined disc method. The isolates were tested for the presence of ESBL blaCTX-M and blaTEM by conventional PCR. Results. Non-repeat 1,302 midstream urine samples were cultured from which 200 different pathogens were isolated. Of the 200 isolates 51 were E. coli. Isolates obtained from Community isolates were 16(37.2) and isolates from in-patients were 27(62.8). Resistant strains were detected to all the 12 antimicrobials tested. Low proportions were sensitive to cephalosporins (cefotaxime and ceftazidime), both recording 8/51(15.7%), and quinolones (nalidixic acid and ciprofloxacin), 7/51(13.7%) and 8/51(15.7%) respectively. The isolates had varied susceptibility to aminoglycosides, with European Scientific Journal March 2017 edition vol.13, No.9 ISSN: 1857 -7881 (Print) e -ISSN 1857 366 low sensitivity of 1/32(2.0%) to gentamicin, but a high proportion of 47/51(92.2%) was sensitive to amikacin. High proportion of the isolates [43/51(84.3%)] were ESBL phenotype, and was found to be significantly associated (p<0.001) with antimicrobial resistance among the isolates. The most prevalent ESBL genotype was BlaTEM with 26 (66.7%) and blaCTX-M 28(71.8%), but 9(23.1%) ESBL phenotypes tested negative for both blaTEM and blaCTX-M genes. 22 (56.4%) of the isolates had both blaTEM and blaCTX-M genes. Conclusion. High proportions of E. coli isolates from urine in Sunyani produce ESBLs. Both blaTEM and blaCTX-M were prevalent and linked to the high levels of drug resistance found in the locality. Increased antibiotic stewardship and stringent infection control measures along with the testing for ESBL must be instituted in the hospital.

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Section: Discussionsupporting

confidence: 91%

Molecular Characterization of Extended-Spectrum Beta-Lactamase Producing Urinary Escherichia coli Isolated in Brong-Ahafo Regional Hospital, Ghana

Wireko

Frimpong

Feglo

2017

ESJ

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“…Ces deux dernières ont occupé 81,23% du total des souches productrices de BLSE. Plusieurs travaux ont également souligné l'émergence observée au niveau de ces deux souches (Kader et Kumar, 2005 ;Al Jasser, 2006 ;Aminzadeh et al, 2007 ;Zarakolu et al, 2007;Tandé et al, 2009). Une action de lutte privilégierait plus le genre Escherichiae et Klebsiellae.…”

Section: Introductionunclassified

Prévalence des souches d’entérobactéries productrices de bêta-lactamases à spectre élargi isolées au Togo et de leur sensibilité aux antibiotiques

Djeri¹,

Karou²,

Tigossou³

et al. 2017

Int. J. Bio. Chem. Sci

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“…In Tanzania (2009-2010), 50.3% (92/183) of K. pneumoniae strains isolated from blood cultures were ESBL producers; 5.5% (5/92) of them were from the community and 94.5% (87/92) from hospitals [13]. In Ghana (2007) and Mali (2003), ESBLs were produced, respectively, by 49.4% and 63.4% of Enterobacteriaceae isolated in community and hospital samples [14,15] [16].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali

Sangare

Maïga

Guindo

et al. 2016

J Infect Dev Ctries

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Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients' blood cultures in two university teaching hospitals of Bamako, Mali. Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results: The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae. Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively). Conclusion: Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.

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Extended-Spectrum β-Lactamase–ProducingEnterobacteriaceaein a Malian Orphanage

Cited by 36 publications

References 9 publications

Molecular Characterization of Extended-Spectrum Beta-Lactamase Producing Urinary Escherichia coli Isolated in Brong-Ahafo Regional Hospital, Ghana

Molecular Characterization of Extended-Spectrum Beta-Lactamase Producing Urinary Escherichia coli Isolated in Brong-Ahafo Regional Hospital, Ghana

Prévalence des souches d’entérobactéries productrices de bêta-lactamases à spectre élargi isolées au Togo et de leur sensibilité aux antibiotiques

Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali

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