Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing–thawing of semen from capuchin monkey

Abstract

We aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.

Effect of various antioxidants and their combinations on bull semen cryopreservation

“…Similarly, 200 or 400 µg CAT also decreased postthawed sperm motility in ram semen [24]. Although 50-100 µg CAT failed to improve motility in fresh extended or cooled monkey semen [36], conversely, 400 µg CAT had positive effects on the postthaw motility of bull [22,37], goat [23], boar [38], and ram [39] sperm.…”

“…Strategic use of antioxidants can mitigate excessive ROS concentrations and enhance sperm quality and function, whereas excessive antioxidant activity could be very detrimental. Although it was suggested that since CAT is an endogenous product, it does not need to be supplemented [36], perhaps apparent differences among studies are related to the species and/or concentrations of CAT used.…”