Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance

Chua, Jie Shi; Chng, Choon-Peng; Moe, Aung Aung Kywe; Tann, Jason Y.; Goh, Eyleen L. K.; Chiam, Keng-Hwee; Yim, Evelyn K.F.

doi:10.1016/j.biomaterials.2014.06.008

Cited by 110 publications

(118 citation statements)

References 56 publications

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“…The aspect ratio of the gratings was shown to be an important determinant of mesenchymal stem cell elongation and alignment [26,27]. Therefore, the spacing and height of the gratings play important roles in the topographical construct that determines cell behaviour and cell fate.…”

Section: Discussionmentioning

confidence: 98%

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Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns

et al. 2015

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Section: Discussionmentioning

confidence: 98%

“…The enhanced neuronal differentiation, which was observed with increasing grating depth, has also been suggested to be due to the depth-sensing ability of the neurites. This ability is facilitated by filopodia adhesion and neurite bending, both of which have been closely associated with neurite alignment and growth [27].…”

Section: Discussionmentioning

confidence: 99%

Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns

et al. 2015

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“…Motivations to model the growth cone cover formation of the extracellular gradient that the growth cone senses427273, axonal pathfinding by the gradient cone74757677787980, the growth cone movement in three-dimensional space25818283, axonal specification during neuronal polarization8485868788 and the gradient sensing based on intracellular reaction23257489 or on Bayesian information approach9091.…”

Section: Discussionmentioning

confidence: 99%

Multi-phasic bi-directional chemotactic responses of the growth cone

Naoki

Nishiyama

Togashi

et al. 2016

Sci Rep

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The nerve growth cone is bi-directionally attracted and repelled by the same cue molecules depending on the situations, while other non-neural chemotactic cells usually show uni-directional attraction or repulsion toward their specific cue molecules. However, how the growth cone differs from other non-neural cells remains unclear. Toward this question, we developed a theory for describing chemotactic response based on a mathematical model of intracellular signaling of activator and inhibitor. Our theory was first able to clarify the conditions of attraction and repulsion, which are determined by balance between activator and inhibitor, and the conditions of uni- and bi-directional responses, which are determined by dose-response profiles of activator and inhibitor to the guidance cue. With biologically realistic sigmoidal dose-responses, our model predicted tri-phasic turning response depending on intracellular Ca2+ level, which was then experimentally confirmed by growth cone turning assays and Ca2+ imaging. Furthermore, we took a reverse-engineering analysis to identify balanced regulation between CaMKII (activator) and PP1 (inhibitor) and then the model performance was validated by reproducing turning assays with inhibitions of CaMKII and PP1. Thus, our study implies that the balance between activator and inhibitor underlies the multi-phasic bi-directional turning response of the growth cone.

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“…Usually, manual or semi-automatic methods for angle detection were used (Chua et al, 2014;Francis et al, 2013;Gros et al, 2010;Mahoney et al, 2005;Tuft et al, 2014;Walsh et al, 2005). Some groups also employed fast Fourier transformation techniques to measure overall alignment over an entire field of view (Corey et al, 2007;Johansson et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

AngleJ: A new tool for the automated measurement of neurite growth orientation in tissue sections

Günther

Schneiders

et al. 2015

Journal of Neuroscience Methods

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Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance

Cited by 110 publications

References 56 publications

Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns

Enhanced differentiation of neural progenitor cells into neurons of the mesencephalic dopaminergic subtype on topographical patterns

Multi-phasic bi-directional chemotactic responses of the growth cone

AngleJ: A new tool for the automated measurement of neurite growth orientation in tissue sections

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