Extension of a set of microsatellite markers for more precise identification of chum salmon Oncorhynchus keta walbaum

Afanasiev, P. K.; Рубцова, Г. А.; Shitova, M. V.; Shaykhaev, E. G.; Zhivotovsky, Lev A.

doi:10.1134/s1022795411110020

Cited by 4 publications

(1 citation statement)

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“…Therefore, microsatellites have become the marker of choice for gene studies and genome evolution for all taxa. Many studies have examined the genetics of salmonid fishes using microsatellite markers in recent years (Kordichevaet al, 2010;Khrustaleva, Volkov, Stocklitskaya, Mugue, & Zelenina, 2010;Afanasiev, Rubtsova, Shitova, Shaĭkhaev, & Zhivotovskii, 2011;Shaikhaev & Zhivotovsky, 2014;Rubtsovaet al, 2016); however, many microsatellite sequences in GenBank for T. arcticus grubei are still unknown. We must screen for more polymorphic microsatellite markers in T. arcticus grubei in order to improve fine-scale population structure, stock management and enhancement, genetic linkage map construction, and molecular marker-assisted breeding.…”

Section: Introductionmentioning

confidence: 99%

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Liu¹,

Liu²,

Shan³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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The population of Xinjiang arctic graylings (Thymallus arcticus grubei) has dramatically declined in China. A total of 22 polymorphic microsatellite loci were isolated and characterized from AFLP products in the Xinjiang arctic grayling. The number of alleles (Na) per locus varied from 3 to 13. Observed (HO) and expected (HE) heterozygosities ranged from 0.60 to 0.97, and from 0.58 to 0.89, respectively. These new microsatellite markers are a helpful tool for genetic analyses, stock management, and resource conservation efforts for T. arcticus grubei. According to the total number of alleles, the total effective number of alleles, and the total number of genotypes, the Fuhai population showed the highest diversity among all the populations while the Fuyun sample was the lowest. Five additional fish species: Red seam bream (Pagrus major), Lenok (Brachymystax lenok), Sea perch (Lateolabrax japonicus), Taimen (Hucho taimen), and Amur grayling (Thymallus grubii) were assessed for cross-species amplification. Three out of the five species showed at least two polymorphic loci. In addition, six loci were found to be polymorphic in at least one species.

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Section: Introductionmentioning

confidence: 99%