Extension of the Lifespan of<i>Caenorhabditis elegans</i>by the Use of Electrolyzed Reduced Water

Yan, Hanxu; Tian, Haijun; Kinjo, Tadamune; Hamasaki, Takeki; Tomimatsu, Kosuke; Nakamichi, Noboru; Teruya, Kiichiro; Kabayama, Shigeru; Shirahata, Sanetaka

doi:10.1271/bbb.100250

Cited by 26 publications

(49 citation statements)

References 35 publications

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“…Diabetes is related to aging. We demonstrated that ERW reduced ROS level of C. elegans and elongated the lifespan of Caenorhabditis elegans (Yan et al 2010). Kim and Kim (2006) used ERW prepared from tap water to demonstrate the anti-diabetes effect of ERW, however, tap water contains dozen of components that make detection of active substance(s) awkward.…”

Section: Discussionmentioning

confidence: 99%

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Hamasaki

Nakamichi³

et al. 2010

Cytotechnology

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Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing b-cells. Here, we showed that electrolyzed reduced water prevented alloxaninduced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic b-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic b-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.Keywords Electrolyzed reduced water Á Alloxan Á Type 1 diabetes mellitus Á Reactive oxygen species Á HIT-T15 cells

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Section: Discussionmentioning

confidence: 99%

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Hamasaki

Nakamichi³

et al. 2010

Cytotechnology

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“…The group of Shirahata studied the activity of ERAW on the nematode Caenorhabtidis elegans [27]. The authors showed that worms cultivated in medium prepared from ERAW had an extended lifespan and that this protects against the life-shortening effect of paraquat.…”

Section: Anti-oxidant Activity Of Eraw Contaminated By Nps During Thementioning

confidence: 99%

Physico-Chemical, Biological and Therapeutic Characteristics of Electrolyzed Reduced Alkaline Water (ERAW)

Henry

Chambron

2013

Water

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Abstract:The consumption of alkaline reduced water produced by domestic electrolysis devices was approved in Japan in 1965 by the Ministry of Health, Labour and Welfare for the cure of gastro-intestinal disorders. Today, these devices are freely available in several countries and can be easily purchased without reserve. The commercial information included with the device recommends the consumption of 1-1.5 L of water per day, not only for gastro-intestinal disorders but also for numerous other illnesses such as diabetes, cancer, inflammation, etc. Academic research in Japan on this subject has been undergoing since 1990 only but has established that the active ingredient is dissolved dihydrogen that eliminates the free radical HO• in vivo. In addition, it was demonstrated that degradation of the electrodes during functioning of the device releases very reactive nanoparticles of platinum, the toxicity of which has not yet been clearly proven. This report recommends alerting health authorities of the uncontrolled availability of these devices used as health products, but which generate drug substances and should therefore be sold according to regulatory requirements.

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“…Another study also showed that the conventional NGM C. elegans culture medium prepared with ERW confers increased resistance to various environmental stresses compared to NGM prepared with distilled water (Park et al, 2012). A previous study showed that water medium prepared with ERW extended mean lifespan of C. elegans (11-41%), but failed to increase maximum lifespan (Yan et al, 2010;Yan et al, 2011). No signifi cant extension of either mean or maximum lifespan by ERW is observed when S-basal medium is used in the lifespan assay (Yan et al, 2010).…”

Section: Discussionmentioning

confidence: 97%

“…ERW = electrolyzed reduced water. grown in ERW accumulate less ROS induced by the oxidativestress inducer paraquat (Yan et al, 2010). Another study also showed that the conventional NGM C. elegans culture medium prepared with ERW confers increased resistance to various environmental stresses compared to NGM prepared with distilled water (Park et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…One study identifi ed tissue-specifi c transcriptional biomarkers of aging through genome-wide transcriptome analysis and showed that supplementation of antioxidants signifi cantly prevents age-related transcriptional changes of biomarkers of aging in a tissue-specifi c way in mice (Park et al, 2009). Interestingly, Yan et al reported that C. elegans grown in water medium prepared with ERW live longer than worms grown in distilled water and have increased resistance to oxidative stress due to the ROS scavenging activity of ERW (Yan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans

Park

2013

Biol. Res.

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Electrolyzed-reduced water (ERW) scavenges reactive oxygen species and is a powerful anti-oxidant. A positive correlation between oxidative stress and aging has been proved in many model organisms. In Caenorhabditis elegans, many long-lived mutants showed reduced fertility as a trade off against longevity phenotype. We aimed to study the eff ect of ERW on oxidative stress, fertility and lifespan of C. elegans. We also investigated the genetic pathway involved in the eff ect of ERW on resistance to oxidative stress and lifespan. We compared lifespan and fertility of worms in media prepared with distilled water and ERW. ERW signifi cantly extended lifespan and increased the number of progeny produced. Then the eff ect of ERW on resistance to oxidative stress and lifespan of long-lived mutants was determined. ERW increased resistance to oxidative stress and lifespan of eat-2, a genetic model of dietary restriction, but had no eff ect on those of age-1, which is involved in insulin/insulin-like growth factor (IGF)-1-like signal. In addition, knockdown of daf-16, the downstream mediator of insulin/IGF-1-like signal, completely prevented the eff ect of ERW on lifespan. These fi ndings suggest that ERW can extend lifespan without accompanying reduced fertility and modulate resistance to oxidative stress and lifespan via insulin/IGF-1-like signal in C. elegans.

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Extension of the Lifespan ofCaenorhabditis elegansby the Use of Electrolyzed Reduced Water

Cited by 26 publications

References 35 publications

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Physico-Chemical, Biological and Therapeutic Characteristics of Electrolyzed Reduced Alkaline Water (ERAW)

Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans

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