Let A, B be two archimedean -algebras and let U,V be two positive linear maps from A to B. We call that the couple (U,V ) is separating with respect to A and B if |a| |b| = 0 in A implies |U (a)||V (b)| = 0 in B. In this paper, we prove that if A is an f -algebra with unit elment e, if B is an -algebra and if (U,V ) is a separating couple with respect to A and B then (U ∼∼ ,V ∼∼ ) , where U ∼∼ (resp V ∼∼ ) is the bi-adjoint of U (resp of V ), is again a separating couple with respect to the order continuous order biduals (A ) n and (B ) n of A and B respectively furnished with their Arens products respectively. Moreover, in the case where B separates the points of B, we give a characterization of any separating couple with respect to A and B.