Extensive evaluations of the cytotoxic effects of gold nanoparticles

Chuang, Shiow-Shuh; Lee, Yi-Hui; Liang, Ruei-Yue; Roam, Gwo-Dong; Zeng, Zih-Ming; Tu, Hsin-Fang; Wang, Shi-Kwun; Chueh, Pin Ju

doi:10.1016/j.bbagen.2013.06.025

Cited by 90 publications

(71 citation statements)

References 52 publications

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“…This result implies that the particles regardless of their physicochemical and surface properties acted through pathways other than apoptosis to inhibit cell growth or that they caused retarded cell proliferation without accompanied massive apoptosis. 55 Previous studies have shown that cells are arrested at different phases of cell cycle or triggered apoptosis mechanisms because of DNA damage caused by external or internal effects. 77−79 Basically, DNA damage triggers a signaling network that induces phosphorylation and consequent activation of ATM (Ataxia telangiectasia mutated), which functions in DNA repair, apoptotic death, and cell cycle arrest.…”

Section: 63mentioning

confidence: 99%

Effect of PEG Grafting Density and Hydrodynamic Volume on Gold Nanoparticle–Cell Interactions: An Investigation on Cell Cycle, Apoptosis, and DNA Damage

Bulmuş

Altınkaya

2016

Langmuir

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In this study, interactions of polyethylene glycol (PEG)-coated gold nanoparticles (AuNPs) with cells were investigated with particular focus on the relationship between the PEG layer properties (conformation, grafting density, and hydrodynamic volume) and cell cycle arrest, apoptosis, and DNA damage. Steric hindrance and PEG hydrodynamic volume controlled the protein adsorption, whereas the AuNP core size and PEG hydrodynamic volume were primary factors for cell uptake and viability. At all PEG grafting densities, the particles caused significant cell cycle arrest and DNA damage against CaCo2 and PC3 cells without apoptosis. However, at a particular PEG grafting density (∼0.65 chains/ nm 2 ), none of these severe damages were observed on 3T3 cells indicating discriminating behavior of the healthy (3T3) and cancer (PC3 and CaCo2) cells. It was concluded that the PEG grafting density and hydrodynamic volume, tuned with the PEG concentration and AuNP size, played an important role in particle−cell interactions.

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Section: 63mentioning

confidence: 99%

Effect of PEG Grafting Density and Hydrodynamic Volume on Gold Nanoparticle–Cell Interactions: An Investigation on Cell Cycle, Apoptosis, and DNA Damage

Bulmuş

Altınkaya

2016

Langmuir

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“…In HeLa cells, exposure to gold nanorods (width and length of 40 × 14 nm and 74 × 14 nm) for 6 h resulted in greater internalization than was observed for spherical nanoparticles (14,30,50,74, and 100 nm sizes)(B. Devika Chithrani et al, 2006;Chithrani and Chan, 2007). One study also indicated that gold nanorods were cytotoxic to most mammalian cells, but the reaction of cells to gold nanorods depended primarily on the cell type rather than on the gold nanorod size and cellular uptake (Chuang et al, 2013). Although the potential toxic impact of gold nanoparticles was controversial, gold nanoparticles were widely used in nanomedicine, such as diagnosis, drug delivery and tumor therapy.…”

Section: Introductionmentioning

confidence: 98%

“…The A549 tumor-cell line, a human lung adenocarcinoma derived by explants-culture of lung cancer tissue from a Caucasian male, was established in 1972 and had characteristic features of Type II cells of the pulmonary epithelium, including lamellar bodies (Lieber et al, 1976). This cell line has been widely used in in vitro cytotoxicity and drug metabolism studies (Beer et al, 2012;Chuang et al, 2013;Foldbjerg et al, 2011;Kimberly A. Foster et al, 1998;Uboldi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

In vitro cytotoxicity of gold nanorods in A549 cells

Tang¹,

Shen²,

Huang³

et al. 2015

Environmental Toxicology and Pharmacology

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“…6,[27][28][29][30][31] Nevertheless, uptake and biocompatibility of several GNPs are controversially discussed. Some studies declare them as nontoxic, 32 but some see their toxic potential 33 to depend on size, surface chemistry, and shape of the GNPs. [34][35][36][37] As a result, it was suggested that negatively charged serum protein-coated GNPs can induce cell damage through extrinsic and intrinsic apoptotic pathways.…”

mentioning

confidence: 99%

Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging

Domey¹,

Teichgräber²,

Hilger³

2015

IJN

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Due to their high X-ray attenuation, gold nanoparticles (GNPs) emerged as preclinical contrast agents by giving high vasculature contrast. For this reason, GNPs are regularly applied for computed tomography (CT) imaging of tumors but not for the visualization of inflammation. The aim of this study was to evaluate the biocompatibility and applicability of preclinical GNPs (AuroVist™) of two different sizes (1.9 nm and 15 nm) for the detection of inflammation-associated phagocytes in early-stage edema. Both GNP variants were stable under in vitro conditions and achieved high micro-CT (mCT) contrast after embedment into agarose. Fifteen-nanometer GNPs were detected after uptake into macrophages via mCT imaging exhibiting higher X-ray contrast than cells treated with 1.9 nm GNPs and untreated ones. Both 1.9 nm and 15 nm GNPs exhibited good biocompatibility on murine macrophages according to ATP and cellular dehydrogenase levels. Reactive oxygen species levels produced by phagocytic cells decreased significantly ( P ≤0.05) after co-incubation with GNPs regardless of the size of the nanoparticle (NP) in comparison to untreated control cells. In vivo mCT studies of inflammation imaging revealed that GNPs with a diameter of 15 nm accumulated within subcutaneous edema 2 hours after injection with a maximum signaling 8 hours postinjection and could be detected up to 48 hours within the edema region. In contrast, 1.9 nm GNPs were not shown to accumulate at the site of the inflammation region and were mostly excreted via the renal system 2–4 hours after injection. In conclusion, our study demonstrated that both GNP variants (1.9 nm and 15 nm) were stable and biocompatible under in vitro conditions. However, only 15 nm NPs have the potential as contrast agent for phagocyte labeling and applications in CT imaging of inflammation on a cellular level.

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Extensive evaluations of the cytotoxic effects of gold nanoparticles

Cited by 90 publications

References 52 publications

Effect of PEG Grafting Density and Hydrodynamic Volume on Gold Nanoparticle–Cell Interactions: An Investigation on Cell Cycle, Apoptosis, and DNA Damage

Effect of PEG Grafting Density and Hydrodynamic Volume on Gold Nanoparticle–Cell Interactions: An Investigation on Cell Cycle, Apoptosis, and DNA Damage

In vitro cytotoxicity of gold nanorods in A549 cells

Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging

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