Externally Applied Vibration at 50 Hz Facilitates Dissolution of Blood Clots In-Vitro

Abstract

Background: Localized Low Frequency Vibration (LLFV) in the low sonic range is utilized for disruption of clots by direct contact in catheter applications. However enhanced clot dissolution whereby an LLFV source is applied external from a clotted lumen (such as to resemble a non-invasive therapy) has not been studied.Objective: To assess the effectiveness of low amplitude extra luminally applied 50 Hz LLFV in dissolution of 1 hr old clots immersed in Heparinized Saline.Methods: One hr old blood clots were each placed within a 3 ml syringe filled with 1.5 ml's of Heparinized Saline. LLFV was then applied against the external surface of each syringe with gentle stroke amplitude (~ 0.5 mm), an intensity within an order of magnitude expected to reach a thrombosed vessel (such as a coronary, pulmonary, cerebral, or peripheral artery), given if a substantially stronger application where to be applied non-invasively across the artery's overlying soft tissue barrier.Results: LLFV yielded statistically superior clot dissolution (25%) in comparison to the non vibrated controls (5%) (p<0.0003).Conclusions: Transluminally applied LLFV (50 Hz) accelerates clot dissolution in vitro. Further study in this area in-vivo appears warranted.

Outcomes of interfacility helicopter transportation in acute stroke care

“…19 In addition, low-frequency (50 Hz) vibrations during HEMS 20,21 might have thrombolytic properties that enhance the efficacy of IV tPA, facilitating reperfusion. 22 In our study, we found that shorter transportation time was associated with higher odds of receiving MT likely because Shorter transportation time was associated with higher odds of receiving MT likely because expedited transport results in higher likelihood of penumbra sustenance.…”

Helicopter “Drip and Ship” Flights Do Not Alter the Pharmacological Integrity of rtPA

“…17 The accelerations and low frequency vibrations associated with HEMs transfer may be synergistic with rtPA and enhance the thrombolysis effectiveness of rtPA. 18,19 The vibration effects in conjunction with thrombolysis have been shown to increase the rate of recanalization compared to thrombolysis alone. 20 On the other hand, vibrations and accelerations could negatively impact the effect of a reconstituted rtPA solution which is not supposed to be shaken per pharmacy practice guidelines.…”

Possible Mechanisms for the Effects of Sound Vibration on Human Health

“…To study the effect of mechanically pulsating a blood clot Hoffman and Gill [ 66 ] conducted an in-vitro study (outside the body) placing a one-hour-old blood clot into a solution of the medication Heparin typically used as a blood thinner to treat heart attacks or angina. The vessel holding the blood clot was then vibrated at 50 Hz for 20 min with a very low amplitude to simulate the amount of vibration that might be transferred into the body from a surface vibration.…”