Extra-corporeal liver BNCT for the treatment of diffuse metastases: What was learned and what is still to be learned

Zonta, A.; Pinelli, T.; Prati, U.; Roveda, L.; Ferrari, Cinzia; Clerici, Anna Maria; Zonta, C.; Mazzini, Giuliano; Dionigi, Paolo; Altieri, S.; Bortolussi, Silva; Bruschi, Piero; Fossati, F.

doi:10.1016/j.apradiso.2009.03.087

Cited by 57 publications

(32 citation statements)

References 7 publications

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“…The thermal neutron irradiation facility has a 100 Â 20 Â 40 cm 3 cavity housed in the modified thermal column of the reactor, and this cavity was used to perform BNCT on the explanted livers of patients affected by multiple metastases (Zonta et al 2009). It is characterized by a noncollimated neutron field with a low contamination of epithermal and fast neutrons (1.9 Â 10 8 cm −2 s −1 for neutron energy between 0.4 eV and 1.6 keV; 4.4 Â 10 7 cm −2 s −1 for neutron energy between 1.6 keV and 17.3 MeV) as well as of photons (Bortolussi et al 2007;Protti et al 2012).…”

Section: Small Animal Irradiation For Bnct Purpose and Neutron-inducementioning

confidence: 99%

Gamma Residual Radioactivity Measurements on Rats and Mice Irradiated in the Thermal Column of a Triga Mark II Reactor for BNCT

et al. 2014

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The current Boron Neutron Capture Therapy (BNCT) experiments performed at the University of Pavia, Italy, are focusing on the in vivo irradiations of small animals (rats and mice) in order to evaluate the effectiveness of BNCT in the treatment of diffused lung tumors. After the irradiation, the animals are manipulated, which requires an evaluation of the residual radioactivity induced by neutron activation and the relative radiological risk assessment to guarantee the radiation protection of the workers. The induced activity in the irradiated animals was measured by high-resolution open geometry gamma spectroscopy and compared with values obtained by Monte Carlo simulation. After an irradiation time of 15 min in a position where the in-air thermal flux is about 1.2 × 10(10) cm(-2) s(-1), the specific activity induced in the body of the animal is mainly due to 24Na, 38Cl, 42K, 56Mn, 27Mg and 49Ca; it is approximately 540 Bq g(-1) in the rat and around 2,050 Bq g(-1) in the mouse. During the irradiation, the animal body (except the lung region) is housed in a 95% enriched 6Li shield; the primary radioisotopes produced inside the shield by the neutron irradiation are 3H by the 6Li capture reaction and 18F by the reaction sequence 6Li(n,α)3H → 16O(t,n)18F. The specific activities of these products are 3.3 kBq g(-1) and 880 Bq g(-1), respectively.

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Section: Small Animal Irradiation For Bnct Purpose and Neutron-inducementioning

confidence: 99%

Gamma Residual Radioactivity Measurements on Rats and Mice Irradiated in the Thermal Column of a Triga Mark II Reactor for BNCT

et al. 2014

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“…This feature enables the obtention of several tissue sections with the same concentration in order to be measured with the different techniques. Liver was chosen because ex-situ BNCT for hepatic metastases is a current project of the Argentinean BNCT group (Miller et al, 2009), based on the first experience at the University of Pavia (Zonta et al, 2009). Kidney was chosen because it accumulates large amounts of boronophenylalanine (BPA), thus it was possible to analyse two tissues with different boron concentrations from each animal.…”

Section: Introductionmentioning

confidence: 99%

Inter-comparison of boron concentration measurements at INFN-University of Pavia (Italy) and CNEA (Argentina)

Portu

Postuma

Gadan

et al. 2015

Applied Radiation and Isotopes

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“…The test was performed in vivo by irradiating the animal inside the thermal column of the TRIGA Mark II reactor in the irradiation facility previously designed for Taormina treatment. [25] The TRIGA maximum power is 250 kW. The irradiation facility has a cross section of 40 20 cm 2 and a length of 1 m and starts at about 1.3 m from the centre of the reactor core.…”

mentioning

confidence: 99%

MRI‐Guided Neutron Capture Therapy by Use of a Dual Gadolinium/Boron Agent Targeted at Tumour Cells through Upregulated Low‐Density Lipoprotein Transporters

Crich¹,

Alberti²,

Szabó³

et al. 2011

Chemistry A European J

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The upregulation of low-density lipoprotein (LDL) transporters in tumour cells has been exploited to deliver a sufficient amount of gadolinium/boron/ligand (Gd/B/L) probes for neutron capture therapy, a binary chemio-radiotherapy for cancer treatment. The Gd/B/L probe consists of a carborane unit (ten B atoms) bearing an aliphatic chain on one side (to bind LDL particles), and a Gd(III)/1,4,7,10-tetraazacyclododecane monoamide complex on the other (for detection by magnetic resonance imaging (MRI)). Up to 190 Gd/B/L probes were loaded per LDL particle. The uptake from tumour cells was initially assessed on cell cultures of human hepatoma (HepG2), murine melanoma (B16), and human glioblastoma (U87). The MRI assessment of the amount of Gd/B/L taken up by tumour cells was validated by inductively coupled plasma-mass-spectrometric measurements of the Gd and B content. Measurements were undertaken in vivo on mice bearing tumours in which B16 tumour cells were inoculated at the base of the neck. From the acquisition of magnetic resonance images, it was established that after 4-6 hours from the administration of the Gd/B/L-LDL particles (0.1 and 1 mmol kg(-1) of Gd and (10)B, respectively) the amount of boron taken up in the tumour region is above the threshold required for successful NCT treatment. After neutron irradiation, tumour growth was followed for 20 days by MRI. The group of treated mice showed markedly lower tumour growth with respect to the control group.

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Extra-corporeal liver BNCT for the treatment of diffuse metastases: What was learned and what is still to be learned

Cited by 57 publications

References 7 publications

Gamma Residual Radioactivity Measurements on Rats and Mice Irradiated in the Thermal Column of a Triga Mark II Reactor for BNCT

Gamma Residual Radioactivity Measurements on Rats and Mice Irradiated in the Thermal Column of a Triga Mark II Reactor for BNCT

Inter-comparison of boron concentration measurements at INFN-University of Pavia (Italy) and CNEA (Argentina)

MRI‐Guided Neutron Capture Therapy by Use of a Dual Gadolinium/Boron Agent Targeted at Tumour Cells through Upregulated Low‐Density Lipoprotein Transporters

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