Extra virgin olive oil and flaxseed oil have no preventive effects on DSS-induced acute ulcerative colitis

Nascimento, Roberto de Paula do; Lima, Amanda Vieira; Oyama, Lila Missae; Paiotti, Ana Paula Ribeiro; Cardili, Leonardo; Pereira, José Aires; Silva, Milena Ferreira; Garofolo, Ingrid Candido; Silveira, Vera Lúcia Flor; Caperuto, Luciana C.

doi:10.1016/j.nut.2020.110731

Cited by 12 publications

(35 citation statements)

References 47 publications

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“…Husbandry conditions were poorly reported, with only 3/19 studies adequately describing number of animals per cage. (34)(35)(36) The American Institute of Nutrition (AIN) purified rodent diet (37,38) with modified fat content was the most common food used (7/19 studies), while remaining studies reported various commercial or non-specific diets. Energy content of the diet was described in 4/19 studies and ranged between 2900 and 3970 kcal/kg, (28,31,33,35) while fat content ranged from 4-10% by weight (Table 2).…”

Section: Environmental and Control Conditionsmentioning

confidence: 99%

“…(49) Among the studies reporting outcomes favouring the intervention, 4 were statistically significant (p< 0.05 -0.001), (29,32,41,42) and 6 studies reported large ESs between 0.97 (95% CI 0.12, 1.82) and 8.73 (95% CI 6.14, 11.33). (29,32,41,45,46,49) Within the remaining studies, one study using DSS mouse models (36) and HLA-B27 rats (39) reported greater weight gain in controls, while a study using TNBS colitis models reported non-significant outcomes with no examinable data. (28) No differences were observed between studies using acute (28,32,36,39,42,46) vs. chronic (41,49) models of colitis (Table 7).…”

Section: Weight Changes Post Studymentioning

confidence: 99%

“…(29,32,41,45,46,49) Within the remaining studies, one study using DSS mouse models (36) and HLA-B27 rats (39) reported greater weight gain in controls, while a study using TNBS colitis models reported non-significant outcomes with no examinable data. (28) No differences were observed between studies using acute (28,32,36,39,42,46) vs. chronic (41,49) models of colitis (Table 7). None of the studies investigated the source of weight loss, thus it is unknown if weight changes were attributed to anorexia, secondary effects of inflammation, altered fluid balance, or other physiological changes.…”

Section: Weight Changes Post Studymentioning

confidence: 99%

“…(33,39,40,(42)(43)(44)(46)(47)(48) ES ranged from -0.34 (95% CI -1.15, 0.48) to -4.63 (95% CI -6.31, -2.95), with 9 of 14 moderate-to-large favouring the intervention. (33,35,39,(42)(43)(44)(46)(47)(48) One study reported outcomes favouring controls (36) which was not statistically significant but had a large ES (+0.95, 95% CI 0.00, 1.89). (Table 11).…”

Section: Inflammatory Cytokinesmentioning

confidence: 99%

“…Nine studies assessed pro-inflammatory IL-1β expressed as quantities in tissue, (31,33,36,44) relative gene expression, (29,39) percentages compared to non-colitis animals, (40) and number of stained cells in sampled colon tissue. (46) Induction of experimental colitis resulted in higher IL-1β expression compared to non-colitis animals in all studies.…”

Section: Interleukin-1β (Il-1β)mentioning

confidence: 99%

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Effects of olives and their constituents on the expression of ulcerative colitis: a systematic review of randomised controlled trials

2021

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Extra virgin olive oil (EVOO) is often associated with anti-inflammatory and antioxidant properties. Its effects on inflammatory conditions such as Ulcerative Colitis (UC) however has yet to be defined. As such, we aimed to conduct a systematic review and meta-analysis of studies investigating olive-based interventions in UC. A comprehensive database search for randomized controlled trials was performed between 9th July 2018 and 16th August 2018. Studies identified from search alerts were included up to the 22nd of June 2020. Both individuals living with UC at any disease stage and murine models of UC were included in this review. No human trials meeting the eligibility criteria were identified, while nineteen animal studies comprised of 849 murine models of UC were included in this review. Pooling of the data could not be performed due to heterogeneous outcomes, however general trends favouring olive-based interventions were identified. Milder disease expression including weight maintenance, reduced rectal bleeding and well-formed stools favouring olive-based interventions was statistically significant in 16/19 studies, with moderate-to-large effect sizes (ESs −0.66 (95% CI −1.56, 0.24) to −12.70 (95% CI −16.8, −8.7)). Olive-based interventions did not prevent the development of colitis-like pathologies in any study. In conclusion, effects of olive-based interventions on murine models of UC appear promising, with milder disease outcomes favouring the intervention in most trials and effect sizes suggesting potential clinical relevance. However, the lack of published randomized controlled human trials warrants further investigation to determine if these effects would translate to individuals living with UC.

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Section: Environmental and Control Conditionsmentioning

confidence: 99%

Section: Weight Changes Post Studymentioning

confidence: 99%

Section: Weight Changes Post Studymentioning

confidence: 99%

Section: Inflammatory Cytokinesmentioning

confidence: 99%

Section: Interleukin-1β (Il-1β)mentioning

confidence: 99%

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Effects of olives and their constituents on the expression of ulcerative colitis: a systematic review of randomised controlled trials

2021

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MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

et al. 2021

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Tight junction proteins play crucial roles in maintaining the integrity of intestinal mucosal barrier. MiRNA-182-5p is capable of targeting claudin-2 which is one of the vital tight junction proteins and the effect and mechanism of miRNA-182-5p was explored here in the DSS-induced colitis model. The pathological conditions were evaluated via hematoxylin and eosin staining. The gene expression level was assessed via PCR. Quantitative immunohistochemistry analysis was performed for the measurement of claudin-2. microRNA.org online tool was used for target gene prediction. Luciferase reporter assay and RNA pull-down assay were performed to detect the target of miRNA-182-5p. The inflammatory and oxidative stress level were measured using corresponding kits. MiRNA-182-5p was highly expressed in colitis model and miRNA-182-5p inhibitor exerted protective effects on colitis induced by DSS in mice. The protective effects includded improvement of pathological changes, increases in anti-inflammation and anti-oxidative genes, and up-regulation of TGF-β1. Claudin-2 mRNA was predicted as the target of miRNA-182-5p, which was validated via luciferase reporter assay and RNA pull-down assay. Claudin-2 overexpression was found in miRNA-182-5p inhibitor group. Consistent with the role of miRNA-182-5p, claudin-2 overexpression also exerted protective effects on DSS-induced colitis in mice. Inhibition of miRNA-182-5p exerted protective effects on colitis via targeting and upregulating claudin-2. The findings in study provide a new therapeutic strategy for colitis treatment and lay the foundation for future study.

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