Extracellular ATP and cell signalling

El-Moatassim, Chakib; Dornand, Jacques; Mani, Jean‐Claude

doi:10.1016/0167-4889(92)90025-7

Cited by 214 publications

(136 citation statements)

References 174 publications

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“…The diversity of cellular responses to ATP and other nucleotides (Dubyak and Fedan, 1990;El-Moatassim et al, 1992) suggested the involvement of multiple receptor types, and the classification of receptor subtypes was originally inferred from pharmacological responses to nucleotides in vitro (Burnstock and Kennedy, 1985).…”

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confidence: 99%

“…Activation of the two major subclasses of G-proteincoupled receptors, P2Y(P2Y1) and P2U(P2Y2)' results in phospholipase C-catalysed hydrolysis of phosphatidylinositol 4,5-bisphosphate and consequent inositol 1,4,5-trisphosphate-mediated release of calcium from intracellular stores (Boarder et al, 1995). A large number of studies have demonstrated the existence of G-proteincoupled P2-purinoceptors on tumour cell lines (Dubyak, 1986;Dubyak et al, 1988;Gonzalez et al, 1989a,b;El-Moatassim et al, 1992;Torres-Marquez et al, 1993), but the implications of purinergic stimulation for the growth and development of cancer have not been thoroughly investigated, as studies of growth control of tumours have tended to focus on peptide growth factors and hormones. A number of investigators have demonstrated inhibition of cancer cell growth by ATP at high concentrations (100 gM to 1 mM) (Weisman et al, 1988;Rapaport, 1990;Dubyak and El-Moatassim, 1993).…”

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confidence: 99%

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Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P2-purinoceptors

Dixon

Wb²,

Fleetwood³

et al. 1997

Br J Cancer

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confidence: 99%

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Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P2-purinoceptors

Dixon

Wb²,

Fleetwood³

et al. 1997

Br J Cancer

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“…In smooth muscles, ATP is released from purinergic nerve endings as a transmitter or from cholinergic and adrenergic nerve endings as a cotransmitter and is involved in the physiological regulation of smooth muscles (for review, see Burnstock, 1990;Olsson & Pearson, 1990;El-Moatassim et al, 1992). Although the responses mediated by ATP are usually inhibitory in oesophagus, stomach, small and large intestines, they are excitatory in vas deferens and urinary bladder (see, Gordon, 1986).…”

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The effects of ATP and α,β‐methylene‐ATP on cytosolic Ca²⁺ level and force in rat isolated aorta

Kitajima

Ozaki

Karaki

1993

British J Pharmacology

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“…It is well known that purinergic receptor activation is responsible for a large variety of metabolic effects (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24). ATP or adenosine or several analogues of purinergic receptors have been noted to affect liver glucose metabolism: i.e.…”

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Exogenous Mg-ATP Induces a Large Inhibition of Pyruvate Kinase in Intact Rat Hepatocytes

Ichaï¹,

El-Mir²,

Nogueira

et al. 2001

Journal of Biological Chemistry

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Mg-ATP infusion in vivo has been reported to be beneficial both to organ function and survival rate in various models of shock. Moreover, a large variety of metabolic effects has been shown to occur in several tissues due to purinergic receptor activation. In the present work we studied the effects of exogenous Mg-ATP in rat liver cells perifused with dihydroxyacetone to investigate simultaneously gluconeogenetic and glycolytic pathways. We found a significant effect on oxidative phosphorylation as characterized by a decrease in oxygen consumption rate and in the cellular ATP-to-ADP ratio associated with an increase in lactate-to-pyruvate ratio. In addition, exogenous Mg-ATP induced rapid and reversible inhibition of both gluconeogenesis and glycolysis. The main effect on gluconeogenesis was located at the level of the fructose cycle, whereas the decrease in glycolysis was due to a strong inhibition of pyruvate kinase. Although pyruvate kinase inhibition induced by exogenous Mg-ATP was allosteric when assessed in vitro after enzyme extraction, we found a large decrease in the apparent maximal velocity when kinetics were assessed in vivo in intact perifused hepatocytes. This newly described short-term regulation of pyruvate kinase occurs only in the intact cell and may open new potentials for the pharmacological regulation of pyruvate kinase in vivo.Numerous publications have emphasized the beneficial role of Mg-ATP infusion in various models of shock or severe trauma in animals. Dysfunction of heart (1), kidney (2), muscle (3), endothelial (4), and immune cells (5, 6) are all improved by this treatment, which significantly increases the survival rate. Because such a benefit was independent of hemodynamic status (4, 7), a metabolic mechanism was proposed (8). Because the liver plays a central role in the metabolic response to such severe illnesses, it may represent a major target for Mg-ATP (9, 10).It is well known that purinergic receptor activation is responsible for a large variety of metabolic effects (11-24). ATP or adenosine or several analogues of purinergic receptors have been noted to affect liver glucose metabolism: i.e. stimulation of glycogenolysis (12, 13), increase (19,25,26) or decrease (11) of gluconeogenesis, and decrease of glycolysis (25). These effects have been related to a cAMP-dependent mechanism (12), a cAMP-independent inositol 3-phosphate/calcium-mediated signaling (13,14,16,18,21,23), a phospholipase C activation (18), or a transcriptional effect (25). It must be noted, however, that if some effects are shared among the adenine nucleotide family, others appear to be specific (12,14,18), suggesting that different signaling pathways may be involved.Because the beneficial effects were observed in vivo with Mg-ATP but not with adenosine (8, 27), we investigated the metabolic effects of exogenous Mg-ATP in rat liver cells perifused with DHA.1 Besides a significant effect on oxidative phosphorylation and on gluconeogenesis, we found that Mg-ATP was responsible for a large inhibition of pyruva...

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Extracellular ATP and cell signalling

Cited by 214 publications

References 174 publications

Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P2-purinoceptors

Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P2-purinoceptors

The effects of ATP and α,β‐methylene‐ATP on cytosolic Ca²⁺ level and force in rat isolated aorta

Exogenous Mg-ATP Induces a Large Inhibition of Pyruvate Kinase in Intact Rat Hepatocytes

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Extracellular ATP and cell signalling

Cited by 214 publications

References 174 publications

Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P2-purinoceptors

Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P2-purinoceptors

The effects of ATP and α,β‐methylene‐ATP on cytosolic Ca2+ level and force in rat isolated aorta

Exogenous Mg-ATP Induces a Large Inhibition of Pyruvate Kinase in Intact Rat Hepatocytes

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The effects of ATP and α,β‐methylene‐ATP on cytosolic Ca²⁺ level and force in rat isolated aorta