Extracellular ATP increases intracellular calcium in cultured adult Schwann cells

Ansselin, A.D.; Davey, D. F.; Allen, David G.

doi:10.1016/s0306-4522(96)00370-3

Cited by 42 publications

(30 citation statements)

References 30 publications

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“…We isolated multiple independent RDA clones for a transmembrane ecto-ATPase (Kegel et al, 1997) and found that it is induced by forskolin treatment of cultured Schwann cells and is expressed in sciatic nerve. Schwann cells respond to extracellular ATP by increasing intracellular calcium concentrations, inducing CREB phosphorylation, yet decreasing adenylyl cyclase activity (Lyons et al, 1994;Robitaille, 1995;Berti-Mattera et al, 1996;Ansselin et al, 1997;Mayer et al, 1997;Stevens and Fields, 2000). However, the response to extracellular ATP is stimulated by agents that increase intracellular cAMP and by axonal contact (Lyons et al, 1994(Lyons et al, , 1995.…”

Section: Forskolin-induced Genesmentioning

confidence: 99%

Modification of representational difference analysis applied to the isolation of forskolin‐regulated genes from Schwann cells

Bermingham

Shumas

Whisenhunt

et al. 2001

J of Neuroscience Research

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Many aspects of the response of Schwann cells to axonal cues can be induced in vitro by the adenylyl cyclase activator forskolin, yet the role of cAMP signaling in regulating Schwann cell differentiation remains unclear. To define better the relationship between cAMP signaling and Schwann cell differentiation, we used a modification of cDNA representational difference analysis (RDA) that permits the analysis of small amounts of mRNA and identified additional genes that are differentially expressed by forskolin-treated and untreated Schwann cells. The genes that we have identified, including MKP3, a regulator of ERK signaling, and the sphingosine-1-phosphate receptor edg3/lp(B3), may play important roles in mediating Schwann cell differentiation.

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Section: Forskolin-induced Genesmentioning

confidence: 99%

Modification of representational difference analysis applied to the isolation of forskolin‐regulated genes from Schwann cells

Bermingham

Shumas

Whisenhunt

et al. 2001

J of Neuroscience Research

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“…Schwann cells isolated from mammalian nerve trunks possess P2Y purinergic metabotropic receptors (primarily P2Y2) that respond to the exogenous application of ATP with a calcium transient derived from the release of this cation from internal stores (Ansselin et al 1997;Berti-Mattera et al 1996;Lyons et al 1994Lyons et al , 1995. Schwann cells "in vivo" also possess P2Y2 receptors, whether these are in close apposition to mammalian sensory endings (Takahashi-Iwanaga and Habara 2002) or amphibian and skate motor-nerve terminals (Green et al 1997;Robitaille 1995).…”

Section: Introductionmentioning

confidence: 99%

Varicosity-Schwann Cell Interactions Mediated by ATP in the Mouse Vas Deferens

Lin

Bennett

2005

Journal of Neurophysiology

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“…At present, there is experimental evidence for the presence of metabotropic ATP receptors. Such P2Y receptors have been found in the paranodal and other cell regions of Schwann cells in situ (Lyons et al 1995;Robitaille, 1995;Green et al 1997;Mayer et al 1997Mayer et al , 1998, as well as in Schwann cells in culture (Lyons et al 1994;Berti-Mattera et al 1996;Ansselin et al 1997;Jeftinija & Jeftinija, 1998). In addition to P2Y receptors, ATP andÏor related nucleotides act at ionotropic (P2X) receptors (for review, see North & Barnard, 1997;Burnstock, 1998;Ralevic & Burnstock, 1998).…”

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confidence: 99%

Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells

Grafe

Mayer

Takigawa

et al. 1999

The Journal of Physiology

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1. The paranodal Schwann cell region is of major importance for the function of a myelinated axon. In the present study we searched for a possible ionotropic effect of extracellular ATP in this Schwann cell compartment. 2. Whole-cell patch-clamp recordings from cultured rat Schwann cells revealed that ATP and 2'-3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP) induced a non-specific cation current. The effect of ATP was much enhanced in a Ca¥-and Mg¥-free solution. ADP, UTP and á,â-methylene adenosine 5'-triphosphate (á,â-meATP) had no effect. 3. Confocal Ca¥ imaging of myelinating Schwann cells in isolated rat spinal roots showed a BzATP-induced rise in the free intracellular Ca¥ concentration in the paranodal Schwann cell cytoplasm whereas á,â-meATP and 2-(methylthio)-adenosine 5'-triphosphate were without effect. In contrast to the known metabotropic effect of UTP on these Schwann cell regions, the BzATP-induced Ca¥ signal was not transient, was unaffected by depletion of intracellular Ca¥ stores and dependent on the presence of extracellular Ca¥. 4. These results suggest that an ionotropic ATP receptor with electrophysiological and pharmacological characteristics of the P2XÝ subtype of nucleotide receptors is functionally active in myelinating Schwann cells of peripheral nerves. Such a receptor might contribute to Schwann cell reactions in nerve injury or neuropathy.

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Extracellular ATP increases intracellular calcium in cultured adult Schwann cells

Cited by 42 publications

References 30 publications

Modification of representational difference analysis applied to the isolation of forskolin‐regulated genes from Schwann cells

Modification of representational difference analysis applied to the isolation of forskolin‐regulated genes from Schwann cells

Varicosity-Schwann Cell Interactions Mediated by ATP in the Mouse Vas Deferens

Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells

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