“…The SIET was designed to detect very weak ion fluxes near cells and tissues, such as H + , Cl - , Na + , and Ca 2+ fluxes near retina cells [ 22 ], kidney cell lines [ 23 ], mouse blastocysts [ 24 ], and embryonic skin of zebrafish [ 21 ], [ 25 ], [ 26 ]. In our previous study, we applied the SIET for the first time to analyze MET channel-mediated Ca 2+ influx at the hair bundle of zebrafish neuromasts [ 15 ]. Using a Ca 2+ -selective microelectrode to deflect hair bundles and simultaneously record the Ca 2+ flux, the inward Ca 2+ flux was detected at stereocilia of hair cells.…”