2013
DOI: 10.1152/ajpcell.00077.2013
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Extracellular Ca2+ and Mg2+ modulate aminoglycoside blockade of mechanotransducer channel-mediated Ca2+ entry in zebrafish hair cells: an in vivo study with the SIET

Abstract: Zebrafish lateral-line hair cells are an in vivo model for studying hair cell development, function, and ototoxicity. However, the molecular identification and properties of the mechanotransducer (MET) channel in hair cells are still controversial. In this study, a noninvasive electrophysiological method, the scanning ion-electrode technique (SIET), was applied for the first time to investigate properties of MET channels in intact zebrafish embryos. With the use of a Ca(2+)-selective microelectrode to deflect … Show more

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Cited by 17 publications
(17 citation statements)
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“…The SIET was designed to detect very weak ion fluxes near cells and tissues, such as H + , Cl - , Na + , and Ca 2+ fluxes near retina cells [ 22 ], kidney cell lines [ 23 ], mouse blastocysts [ 24 ], and embryonic skin of zebrafish [ 21 ], [ 25 ], [ 26 ]. In our previous study, we applied the SIET for the first time to analyze MET channel-mediated Ca 2+ influx at the hair bundle of zebrafish neuromasts [ 15 ]. Using a Ca 2+ -selective microelectrode to deflect hair bundles and simultaneously record the Ca 2+ flux, the inward Ca 2+ flux was detected at stereocilia of hair cells.…”
Section: Discussionmentioning
confidence: 99%
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“…The SIET was designed to detect very weak ion fluxes near cells and tissues, such as H + , Cl - , Na + , and Ca 2+ fluxes near retina cells [ 22 ], kidney cell lines [ 23 ], mouse blastocysts [ 24 ], and embryonic skin of zebrafish [ 21 ], [ 25 ], [ 26 ]. In our previous study, we applied the SIET for the first time to analyze MET channel-mediated Ca 2+ influx at the hair bundle of zebrafish neuromasts [ 15 ]. Using a Ca 2+ -selective microelectrode to deflect hair bundles and simultaneously record the Ca 2+ flux, the inward Ca 2+ flux was detected at stereocilia of hair cells.…”
Section: Discussionmentioning
confidence: 99%
“…Using a Ca 2+ -selective microelectrode to deflect hair bundles and simultaneously record the Ca 2+ flux, the inward Ca 2+ flux was detected at stereocilia of hair cells. As little as 10 µM aminoglycoside antibiotics (neomycin and gentamicin) was found to effectively block Ca 2+ influx within 10 min, showing the SIET to be a sensitive approach for functionally assaying MET channels in intact zebrafish [ 15 ]. In this study, the MET channel mediated Ca 2+ influx measured with the SIET was significantly decreased in zAE1b morphant ( Fig.…”
Section: Discussionmentioning
confidence: 99%
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