Extracellular Ca2+-dependent enhancement of cytocidal potency of zoledronic acid in human oral cancer cells

Inoue, Shuichiro; Arai, Naoya; Tomihara, Kei; Takashina, Michinori; Hattori, Yoshiyuki; Noguchi, Makoto

doi:10.1016/j.ejphar.2015.04.032

Cited by 5 publications

(8 citation statements)

References 40 publications

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“…Then, cells were washed with PBS, harvested by trypsinization, centrifuged, and resuspended in binding buffer from the kit containing Annexin v-FITC and PI. The samples were analyzed using a flow cytometer (FACSCanto II, Becton-Dickinson, Franklin Lakes, NJ, USA), as reported earlier (28).…”

Section: Methodsmentioning

confidence: 99%

Different effect of resveratrol to induction of apoptosis depending on the type of human cancer cells

Takashina

Inoue

Tomihara

et al. 2017

International Journal of Oncology

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The effect of resveratrol on various human cancer cells was investigated with special focus on apoptotic cell death, in an attempt to further characterize its mechanism of action. There were great differences in the anti-viability effect of resveratrol between different types of human cancer cells. While the inhibition of cell viability by resveratrol was marked in U937 and MOLT-4 leukemia cells, resveratrol moderately inhibited cell viability in MCF-7 breast, HepG2 liver, and A549 lung cancer cells, and the effect was slight on cell viability in Caco-2, HCT116, and SW480 colon cancer cells. Following resveratrol treatment, U937 and MOLT-4 markedly increased the population of late apoptotic cells but MCF-7 and HepG2 underwent apoptosis with an increased population of early apoptosis, and resveratrol-induced DNA fragmentation was observed only in leukemic cells. Activation of sirtuin 1 and adenosine-monophosphate-activated protein kinase was not responsible for resveratrol-induced cancer cell death. Instead, resveratrol significantly reduced Akt activation with the downregulation of H-Ras, resulting in facilitation of Bax translocation to mitochondria in leukemic cells. This study suggests that resveratrol can induce apoptotic cell death in human leukemic cells to a greater extent than in human solid tumor cells via reducing Akt activation due to Ras downregulation.

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Section: Methodsmentioning

confidence: 99%

Different effect of resveratrol to induction of apoptosis depending on the type of human cancer cells

Takashina

Inoue

Tomihara

et al. 2017

International Journal of Oncology

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“…Bisphosphonates (BPs) are first-line drugs for osteolytic diseases, including osteoporosis, Paget's disease, and cancerinduced hypercalcemia (4). BPs act as potent osteoclastic bone resorption inhibitors by inhibiting osteoclast activity and inducing their apoptosis (4)(5)(6). Zoledronic acid (ZA) is a third-generation, nitrogen-containing BP and is potentially a greater inhibitor of bone resorption (4,7).…”

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confidence: 99%

“…BPs act as potent osteoclastic bone resorption inhibitors by inhibiting osteoclast activity and inducing their apoptosis (4)(5)(6). Zoledronic acid (ZA) is a third-generation, nitrogen-containing BP and is potentially a greater inhibitor of bone resorption (4,7). ZA is widely used to prevent metastatic cancer-induced bone diseases (4,7).…”

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confidence: 99%

“…Zoledronic acid (ZA) is a third-generation, nitrogen-containing BP and is potentially a greater inhibitor of bone resorption (4,7). ZA is widely used to prevent metastatic cancer-induced bone diseases (4,7). Moreover, the direct antitumor activity of ZA has been reported in many cancer cell types in vitro, including oral squamous cell carcinoma (OSCC) cells, demonstrating significant growth inhibition and apoptosis induction (4,(7)(8)(9).…”

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confidence: 99%

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Evaluation of a New Hydroxyapatite Nanoparticle as a Drug Delivery System to Oral Squamous Cell Carcinoma Cells

et al. 2018

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Background/Aim: Due to its abilities of substance adsorption and intracellular transportation, hydroxyapatite is a potential carrier in drug delivery systems (DDS). This in vitro study investigated whether newly-developed, highlydispersive calcined hydroxyapatite nanoparticles with an average grain diameter of 20 nm (nano-SHAP) were suitable as a DDS for the drugs zoledronic acid (ZA), cisplatin, and carboplatin. Material and Methods: The effects of drugbearing nano-SHAP on cell proliferation were assessed using three human oral squamous cell carcinoma cell lines (HSC-4, KOSC, and SAS) and one human breast cancer cell line (MCF-7). Results: Nano-SHAP alone did not affect proliferation of any cell line until a concentration of 1 μg/ml was reached. Although the effective concentration of ZA in ZA-bearing nano-SHAP differed, it inhibited cell proliferation better than ZA alone. Cisplatin and carboplatin-bearing nano-SHAP had the same effect as these drugs alone. Conclusion: The nano-SHAP system is of potential use as a drug delivery system. Hydroxyapatite (HAP), which has a structural formula of Ca 10 (PO 4 ) 6 (OH) 2 , is the principal inorganic constituent of human bones and teeth (1, 2). HAP is widely used in medical applications, including treating bone defects, tissue engineering systems, and bioactive coatings on metallic osseous implants (1-3). Hydroxyapatite nanoparticle (nano-HAP) structures have a large surface area, high loading capacity, and are capable of intracellular transportation. As a result, nano-HAP has been explored as a therapeutic molecule for drug delivery systems (DDS) (2, 3). Since nano-HAP has 6715

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“…Cells were seeded in 96‐well plates at a density of 1.5 × 10 4 cells/well and cultured in the absence or presence of 5 µM AST for 12 hours. They were then treated with various concentrations (100 µM, 200 µM, or 400 µM) of cisplatin (Waco Pure Chemical Industries Ltd.) . After 48 hours, the viability of the adherent cells was measured by adding tetrazolium salt, WST‐1(4‐[3‐(4‐iodophenyl)‐2‐(4‐nitrophenyl)‐2H‐5‐tetrazolio]‐1,3‐benzene disulphonate) premix (Takara Bio), to each well.…”

Section: Methodsmentioning

confidence: 99%

Astaxanthin ameliorates cisplatin‐induced damage in normal human fibroblasts

Yamaguchi

Tomihara

Heshiki

et al. 2019

Oral Science International

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Objective Oral mucositis is a common adverse side effect of cancer chemotherapy. Its management is of vital importance during cancer treatment. Oxidative stress against normal cells, including epithelial and stromal cells, has been implicated in the pathogenesis of chemotherapy‐induced oral mucositis. Fibroblasts may play a major role in the repair of oral mucosal tissue after chemotherapy. Astaxanthin (AST), a natural xanthophyll carotenoid, has sparked interest because of its strong antioxidant and anti‐inflammatory properties. This study illustrates the protective effects of astaxanthin on normal fibroblasts damaged by chemotherapy. Methods Human normal fibroblasts were exposed to cisplatin. The cells were then assessed for cell viability, apoptosis, production of reactive oxygen species, migratory activity, and expression of platelet‐derived growth factor receptors (PDGFRs) in the absence or presence of astaxanthin. Results Astaxanthin protected fibroblasts from the side effects of cisplatin, such as reduction in cell viability, apoptotic cell death, generation of reactive oxygen species, reduction in migration activity, and reduced expression of PDGFRs. Conclusions Our results indicate a broad range of protection from cisplatin damage in fibroblasts by astaxanthin, implying its possible use as a novel therapeutic agent against chemotherapy‐induced oral mucositis.

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Extracellular Ca2+-dependent enhancement of cytocidal potency of zoledronic acid in human oral cancer cells

Cited by 5 publications

References 40 publications

Different effect of resveratrol to induction of apoptosis depending on the type of human cancer cells

Different effect of resveratrol to induction of apoptosis depending on the type of human cancer cells

Evaluation of a New Hydroxyapatite Nanoparticle as a Drug Delivery System to Oral Squamous Cell Carcinoma Cells

Astaxanthin ameliorates cisplatin‐induced damage in normal human fibroblasts

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