Extracellular calcium is involved in egg yolk-induced head-to-head agglutination of bull sperm

Yang, Da-Lei; McMillan, A.G.; Standley, N.T.; Shannon, Patrick; Xu, Z.Z.

doi:10.1016/j.theriogenology.2012.06.021

Cited by 13 publications

(15 citation statements)

References 19 publications

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“…As previously shown for bull (Yang et al 2012) and boar (Harayama et al 1998(Harayama et al , 2000a) spermatozoa, contact with HCO 3 K , Ca 2C and albumin induced rapid headto-head agglutination in suspended sperm. For this reason, we hypothesized that reduced sperm-oviduct binding in combined HCO 3 K and albumin conditions was a product of rapid head-to-head agglutination of stallion sperm.…”

Section: Csupporting

confidence: 72%

“…Sperm agglutination is a common event during manipulation of sperm from many mammalian species and is a problem during in vitro sperm studies because it interferes with accurate sperm assessment. Sperm agglutination occurs between spermatozoa with intact plasma and acrosome membranes (Yang et al 2012). Typically head-to-head agglutination takes place when sperm is exposed in vitro to: i) washing media (ram (Dott & Walton 1960)), ii) fluids from the female genital tract (bull (Lindahl 1966)), iii) divalent cations (rabbit (Bedford 1970), bull (Lindahl 1973), iv) bovine serum in combination with semen extender (bull (Senger & Saacke 1976)) and v) IVF medium (boar (Harayama et al 1998(Harayama et al , 2000a).…”

Section: Discussionmentioning

confidence: 99%

“…Typically head-to-head agglutination takes place when sperm is exposed in vitro to: i) washing media (ram (Dott & Walton 1960)), ii) fluids from the female genital tract (bull (Lindahl 1966)), iii) divalent cations (rabbit (Bedford 1970), bull (Lindahl 1973), iv) bovine serum in combination with semen extender (bull (Senger & Saacke 1976)) and v) IVF medium (boar (Harayama et al 1998(Harayama et al , 2000a). Divalent cations (including Ca 2C , Mg 2C and Mn 2C ) activate an ATPdependent surface reaction which triggers head-to-head sperm agglutination (Harayama et al 1998, 2000a, Yang et al 2012. Alternatively, sperm head-to-head agglutination is also observed in an early stage of the capacitation process in vitro when anti-agglutinin is removed from the sperm surface (Lindahl & Sjoblom 1981, Harayama et al 2000b.…”

Section: Discussionmentioning

confidence: 99%

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Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm–oviduct binding

Leemans¹,

Gadella²,

Stout³

et al. 2016

Reproduction

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In many species, sperm binding to oviduct epithelium is believed to be an essential step in generating a highly fertile capacitated sperm population primed for fertilization. In several mammalian species, this interaction is based on carbohydrate-lectin recognition.D-galactose has previously been characterized as a key molecule that facilitates sperm-oviduct binding in the horse. We used oviduct explant and oviduct apical plasma membrane (APM) assays to investigate the effects of various carbohydrates; glycosaminoglycans; lectins; S-S reductants; and the capacitating factors albumin, Ca 2C and HCO 3 K on sperm-oviduct binding in the horse. Carbohydratespecific lectin staining indicated that N-acetylgalactosamine, N-acetylneuraminic acid (sialic acid) and D-mannose or D-glucose were the most abundant carbohydrates on equine oviduct epithelia, whereas D-galactose moieties were not detected. However, in a competitive binding assay, sperm-oviduct binding density was not influenced by any tested carbohydrates, glycosaminoglycans, lectins or D-penicillamine, nor did the glycosaminoglycans induce sperm tail-associated protein tyrosine phosphorylation. Furthermore, N-glycosidase F (PNGase) pretreatment of oviduct explants and APM did not alter sperm-oviduct binding density. By contrast, a combination of the sperm-capacitating factors albumin and HCO 3 K severely reduced (O10-fold) equine sperm-oviduct binding density by inducing rapid head-to-head agglutination, both of which events were independent of Ca 2C and an elevated pH (7.9). Conversely, neither albumin and HCO 3 K nor any other capacitating factor could induce release of oviduct-bound sperm. In conclusion, a combination of albumin and HCO 3 K markedly induced sperm head-to-head agglutination which physically prevented stallion sperm to bind to oviduct epithelium.

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Section: Csupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm–oviduct binding

Leemans¹,

Gadella²,

Stout³

et al. 2016

Reproduction

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“…Further indirect evidence included the stronger association of spermatozoa, which required higher doses of PEN (1 mM compared to 0.25 mM) to reverse agglutination, when db-cAMP and PDE inhibitors were included in the TALP medium. However, unlike the previous studies (Lindahl & Sjö blom 1981, Harayama et al 1998, Harayama & Kato 2002, Yang et al 2012, agglutination did not rely on the presence of calcium as the omission of calcium from the TALP medium had no effect on sperm agglutination (Fig. 2).…”

Section: Discussioncontrasting

confidence: 52%

Penicillamine prevents ram sperm agglutination in media that support capacitation

Leahy¹,

Rickard²,

Aitken³

et al. 2016

Reproduction

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Ram spermatozoa are difficult to capacitate in vitro. Here we describe a further complication, the unreported phenomenon of headto-head agglutination of ram spermatozoa following dilution in the capacitation medium Tyrodes plus albumin, lactate and pyruvate (TALP). Sperm agglutination is immediate, specific and persistent and is not associated with a loss of motility. Agglutination impedes in vitro sperm handling and analysis. So the objectives of this study were to investigate the cause of sperm agglutination and potential agents which may reduce agglutination. The percentage of non-agglutinated, motile spermatozoa increased when bicarbonate was omitted from complete TALP suggesting that bicarbonate ions stimulate the agglutination process. D-penicillamine (PEN), a nucleophilic thiol, was highly effective at reducing agglutination. The inclusion of 250 mM PEN in TALP reduced the incidence of motile, agglutinated spermatozoa from 76.7G2.7% to 2.8G1.4%. It was then assessed if PEN (1 mM) could be included in existing ram sperm capacitation protocols (TALP C1 mM dibutyryl cAMP, caffeine and theophylline) to produce spermatozoa that were simultaneously capacitated and non-agglutinated. This protocol resulted in a sperm population which displayed high levels of tyrosine phosphorylated proteins and lipid disordered membranes (merocyanine-540) while remaining motile, viable, acrosome-intact and non-agglutinated. In summary, PEN (1 mM) can be included in ram sperm capacitation protocols to reduce sperm agglutination and allow for the in vitro assessment of ram sperm capacitation.

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“…The published data suggested that addition of amino acids to extender improved post-thawing sperm motility, sperm viability, acrosome integrity and membrane integrity in goat glutamine, different concentrations of Taurine, Cysteine and Trehalose, Cysteine, glycine, L-cysteine DL-alanine Glycine L-glutamine L-tryptophan, addition of glycine to Standard extender Tris with concentration 25 mm to bull semen improve post-thawing sperm motility, viability, decrease abnormality and dramatically improve the conception rate, ram [36][37][38][39][40]. However, by which mechanism these amino acid components protect spermatozoa during the freezing-thawing process, have not clearly understood and are still unclear.…”

Section: Discussionmentioning

confidence: 99%

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Mohammed¹,

Ahmed²

2017

Andrology

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Objectives: As very few studies were done on the freezability of pure Egyptian cattle bull sperm. So, we designed this study to evaluate the individual variations in freezability of native bull semen extended in Tris based diluent and sodium citrate-based diluent. Methods:Semen was collected by artificial vagina, examined at once in farm laboratory. Only semen samples fit the minimum parameters are extended in two extenders first the universal one (TRIS) and second modified Sodium citrate extender by adding glycerol (CU-16) which created at Cornell University to evaluate the individual bull variation and interaction between extender and bull.Results: Post-Thawing individual sperm motility, live, abnormal, acrosome integrity percentage was evaluated in addition to Hypo-Osmotic Swelling test (HOS). The highest value for motility, live abnormal and acrosome percentage were 44.00 ± 1.12, 52.25 ± 1.60, 21.25 ± 0.81 and 62.80 ± 2.58 from bull 2, 1, 3 and 2, respectively for semen extended in TRIS, and 42.25 ± 1.61, 52.00 ± 1.76, 22.15 ± 0.85 and 57.40 ± 3.07, from bull 1, 1, 4, 3, respectively for semen extended in CU-16. The results of HOS were 59.25 ± 1.76 and 55.95 ± 2.13 from bull 1 extended in TRIS and CU-16, respectively. Conclusion:A significant variation (p<0.05) in tested parameter was clear when semen extended in TRIS extender with the absence of such variation when semen extended in CU-16 except in live sperm percentage. With ignoring the extender effect a clear significant variations were detected between bulls in all tested parameters.

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Extracellular calcium is involved in egg yolk-induced head-to-head agglutination of bull sperm

Cited by 13 publications

References 19 publications

Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm–oviduct binding

Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm–oviduct binding

Penicillamine prevents ram sperm agglutination in media that support capacitation

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

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