Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Abstract

Objectives: As very few studies were done on the freezability of pure Egyptian cattle bull sperm. So, we designed this study to evaluate the individual variations in freezability of native bull semen extended in Tris based diluent and sodium citrate-based diluent. Methods:Semen was collected by artificial vagina, examined at once in farm laboratory. Only semen samples fit the minimum parameters are extended in two extenders first the universal one (TRIS) and second modified Sodium citrate extender by adding glycerol (CU-16) which created at Cornell University to evaluate the individual bull variation and interaction between extender and bull.Results: Post-Thawing individual sperm motility, live, abnormal, acrosome integrity percentage was evaluated in addition to Hypo-Osmotic Swelling test (HOS). The highest value for motility, live abnormal and acrosome percentage were 44.00 ± 1.12, 52.25 ± 1.60, 21.25 ± 0.81 and 62.80 ± 2.58 from bull 2, 1, 3 and 2, respectively for semen extended in TRIS, and 42.25 ± 1.61, 52.00 ± 1.76, 22.15 ± 0.85 and 57.40 ± 3.07, from bull 1, 1, 4, 3, respectively for semen extended in CU-16. The results of HOS were 59.25 ± 1.76 and 55.95 ± 2.13 from bull 1 extended in TRIS and CU-16, respectively. Conclusion:A significant variation (p<0.05) in tested parameter was clear when semen extended in TRIS extender with the absence of such variation when semen extended in CU-16 except in live sperm percentage. With ignoring the extender effect a clear significant variations were detected between bulls in all tested parameters.

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

“…The lack of individual variation in sperm abnormalities in fresh semen is in accordance with similar research on the Egyptian bull [13] and Saloia ram [30]. Sperm abnormalities might occur as the result of disturbances in the stage of spermatogenesis or the maturation process in the epididymis.…”

“…The sperm viability findings for frozen sperm are similar to those of Mohammed and Ahmed [13] for pure Egyptian bulls, Marques et al [30] for Saloia rams, and Lukman et al [36] for Bali bulls. The individual variation in sperm viability found in this study might be caused by the proportion of lipid and cholesterol in the sperm membrane.…”

“…The finding of individual variation in sperm abnormalities in frozen semen is in disagreement with Mohammed and Ahmed [13] and Marques et al [30] who found no individual variation in sperm abnormalities in pure Egyptian bulls and Black Bengal bucks, respectively. The rate of sperm abnormalities obtained in this study was lower than that found by Carreira et al [42].…”

“…Individual variation in sperm motility in frozen semen has also been found for Egyptian bulls [13] and in Saloia rams [30]. The range of sperm motility that was obtained in this study indicates that all bulls had sperm with good freezability.…”

“…The variation of individual bulls is one aspect of genetic factors. Research regarding the influence of individual factors on the quality of semen has been widely reported for bulls [13], stallions [14], bucks [15], rams [16], and elephants [17].…”