Extracellular dextranase activity produced by human oral strains of the genus Bifidobacterium

Kaster, A G; Brown, Lee R.

doi:10.1128/iai.42.2.716-720.1983

Cited by 18 publications

(7 citation statements)

References 27 publications

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“…The data contained in this report should not be regarded as a precise definition of the cariogenic microflora associated with the tooth surfaces sampled. For example, we have isolated a variety of microorganisms, including Rothia dentocariosa, from both noncarious and carious sites (unpublished data) as well as dextranolytic strains of bifidobacteria from root lesions (12). Furthermore, the data presented here and in other studies of root caries (5,8,10,19,21) should not be considered entirely comparable because of differences in both the sampling and the culture methods.…”

Section: Discussionmentioning

confidence: 87%

Quantitative comparisons of potentially cariogenic microorganisms cultured from noncarious and carious root and coronal tooth surfaces

Brown¹,

Billings²,

Kaster³

1986

Infect Immun

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Potentially cariogenic microorganisms cultured from noncarious and carious root and coronal (enamel) surfaces were quantitatively compared in patients 22 to 84 years of age (mean, 52 years). We collected 150 plaque specimens from 26 in situ teeth with initial root lesions and from 25 extracted teeth with advanced root lesions. The frequencies of isolation of Streptococcus mutans, Actinomyces viscosus, and Lactobacillus spp. were, respectively, 94, 72, and 51% at the noncarious root site; 98, 71, and 54% at the root lesion; 84, 61, and 44% at the noncarious enamel site; and 100, 66, and 90% at the enamel lesion. The streptococci made up the largest mean proportion of the total anaerobic cultivable microflora, ranging from 31.2% at the noncarious enamel site to 37.6% at the root lesion, while S. mutans varied between 18% at the noncarious enamel and root surfaces and approximately 24% at both the enamel and root lesions. The proportion of actinomyces ranged from 12.3% at the root lesion to 23.6% at the noncarious root site, while A. viscosus varied from 7.8% at the root lesion to 15.1% at the noncarious root site. The largest mean proportion of lactobacilli (4.2%) was recovered at the enamel lesion site. Proportions of Candida spp. made up less than 0.1% at all sites. Proportions of microorganisms did not differ significantly between noncarious enamel and root sites, but the noncarious coronal and root sites had higher (P less than 0.05) proportions of actinomyces than did the root lesion. Also, enamel lesions had a greater (P less than 0.05) percentage of Lactobacillus spp. than did root lesions. The number of streptococci recovered from the root lesion was greater (P less than 0.01) than the number of actinomyces at the same site. S. mutans was recovered from initial root lesions in greater numbers (P less than 0.001) than were actinomyces and lactobacilli. The number of S. mutans recovered at the initial root lesions was greater (P less than 0.01) than that recovered from the advanced root lesions.

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Section: Discussionmentioning

confidence: 87%

Quantitative comparisons of potentially cariogenic microorganisms cultured from noncarious and carious root and coronal tooth surfaces

Brown¹,

Billings²,

Kaster³

1986

Infect Immun

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“…Bifidobacteria were probably not the main cause of this extended metabolism, as they were absent in water kefir B. Although there was no evidence for the degradation of the grain EPS, dextranase activity has already been shown in certain bifidobacterial (Kaster and Brown 1983) and LAB species (Picozzi et al 2015).…”

Section: Discussionmentioning

confidence: 97%

The water kefir grain inoculum determines the characteristics of the resulting water kefir fermentation process

Laureys

Vuyst

2017

J Appl Microbiol

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“…Supportive of these findings, Mantzourani et al have also identified B. dentium, P. denticolens , and S. inopinata from the caries samples of primary teeth [ 61 ]. An intriguing characteristic shared by these species is their ability to degrade complex carbohydrates, including dextran [ 62 ] which potentiates the production of demineralizing acids within the cariogenic biofilm, even in the absence of fermentable carbohydrates [ 62 ]. Furthermore, another recent study has reported the synergism in acidogenicity in dual-species biofilms of P. denticolens, S. inopinata , and B. dentium , with S. mutans [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC)

Fakhruddin

Samaranayake

Hamoudi

et al. 2022

Journal of Oral Microbiology

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Background Severe-early childhood caries (S-ECC) a global problem of significant concern, commonly manifest on the occlusal, and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches the compositional differences, if any, in the microbiota of such lesions is unknown. Methods Deep-dentine caries samples from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries-code 5/6, (ICDAS classification) were evaluated. Employing two primer pools, we amplified and compared the bacterial 16S rRNA gene sequences of the seven hypervariable regions (V2—V4 and V6—V9) using NGS-based assay. Results Bray-Curtisevaluation indicated that occlusal lesions (OL) had a more homogeneous community than the proximal lesions (PL) with significant compositional differences at the species level ( p = 0.01; R- 0.513). Together, the occlusal and proximal niches harbored 263 species, of which 202 (76.8%) species were common to both , while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal and occlusal niches, respectively. The most commonl genera at both niches included Streptococcus, Prevotella , and Lactobacillus. S. mutans was predominant in PL ( p ≤ 0.05), and Atopobium parvulum ( p = 0.01) was predominant in OL. Conclusions Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.

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Extracellular dextranase activity produced by human oral strains of the genus Bifidobacterium

Cited by 18 publications

References 27 publications

Quantitative comparisons of potentially cariogenic microorganisms cultured from noncarious and carious root and coronal tooth surfaces

Quantitative comparisons of potentially cariogenic microorganisms cultured from noncarious and carious root and coronal tooth surfaces

The water kefir grain inoculum determines the characteristics of the resulting water kefir fermentation process

Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC)

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