Extracellular enzymes of cold-adapted bacteria from Arctic sea ice, Canada Basin

Yu, Yong; Li, Huirong; Zeng, Yangsu; Chen, Bo

doi:10.1007/s00300-009-0654-x

Cited by 63 publications

(42 citation statements)

References 48 publications

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“…We agree with Yu et al (2009), who indicated that the evolutionary progress of enzyme adaptation to cold temperatures is not consistent with that of bacterial physiology and metabolism; however, the entire environment must be taken into account. In the Antarctic environment, the organic matter in lakes and gullies plays a central role in the geochemical cycle of bioelements through their production, transformation, transport and/or decomposition.…”

Section: Discussionsupporting

confidence: 79%

Antarctic bacterial isolates that produce cold-active extracellular proteases at low temperature but are active and stable at high temperature

MartÃnez-Rosales

Castro‐Sowinski

2011

Polar Research

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We report the isolation and identification of bacteria that produce extracellular cold-active proteases, obtained from water samples collected near the Uruguayan Antarctic Base on King George Island, South Shetlands. The bacteria belonged to the genera Pseudomonas (growth between 4 and 30 °C) and Flavobacterium (growth between 4 and 18 °C). In all cases, extracellular protease production was evident when reaching the stationary phase at 18 and 4 °C but was not detected at 30 °C. The zymogram revealed the secretion of one extracellular protease per isolate, each with different relative electrophoretic mobility. The extracellular proteases produced at 4 °C showed thermal activity and stability at 30 °C. Both activity and stability at a temperature higher that 10 °C have no physiological meaning because the isolates do not experience such temperatures in the Antarctic environment; however, the possible ecological value of cold-active and -stable extracellular proteases is discussed

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Section: Discussionsupporting

confidence: 79%

Antarctic bacterial isolates that produce cold-active extracellular proteases at low temperature but are active and stable at high temperature

MartÃnez-Rosales

Castro‐Sowinski

2011

Polar Research

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“…The 110 isolates used to screen EPS-producing isolates were previously isolated from Arctic sea ice of the Canada Basin (77°30=N to 80°12=N) (16). EPS-producing isolates were screened by using the method of Freeman et al (17) , and artificial seawater (pH 7.5).…”

Section: Screening Of Eps-producing Isolatesmentioning

confidence: 99%

Structure and Ecological Roles of a Novel Exopolysaccharide from the Arctic Sea Ice Bacterium Pseudoalteromonas sp. Strain SM20310

Liu

Chen

et al. 2013

Appl Environ Microbiol

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The structure and ecological roles of the exopolysaccharides (EPSs) from sea ice microorganisms are poorly studied. Here we show that strain SM20310, with an EPS production of 567 mg liter ؊1 , was screened from 110 Arctic sea ice isolates and identified as a Pseudoalteromonas strain. The EPS secreted by SM20310 was purified, and its structural characteristics were studied. The predominant repeating unit of this EPS is a highly complicated ␣-mannan with a molecular mass greater than 2 ؋ 10 6 Da. The backbone of the EPS consists of 2-␣-, 6-␣-mannosyl residues, in which a considerable part of the 6-␣-mannosyl residues are branched at the 2 position with either single t-mannosyl residues or two mannosyl residues. The structure of the described EPS is different from the structures of EPSs secreted by other marine bacteria. Analysis of the ecological roles of the identified EPS showed that the EPS could significantly enhance the high-salinity tolerance of SM20310 and improve the survival of SM20310 after freeze-thaw cycles. These results suggest that the EPS secreted by strain SM20310 enables the strain to adapt to the sea ice environment, which is characterized by low temperature, high salinity, and repeated freeze-thaw cycles. In addition to its functions in strain SM20310, this EPS also significantly improved the tolerance of Escherichia coli to freeze-thaw cycles, suggesting that it may have a universal impact on microorganism cryoprotection.

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“…Waters since, for example, only a few strains isolated from Arctic sea ice have been found 9 to be able to hydrolyze carbohydrates (Yu et al 2009). The analysis of bacterial 10 phylogenetic diversity in our samples will provide a further clue to the possible influence of 11 ice bacteria to the increased carbohydrate utilization in Polar Sea Waters.…”

Section: ) 18mentioning

confidence: 99%

The impact of ice melting on bacterioplankton in the Arctic Ocean

et al. 2010

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Extracellular enzymes of cold-adapted bacteria from Arctic sea ice, Canada Basin

Cited by 63 publications

References 48 publications

Antarctic bacterial isolates that produce cold-active extracellular proteases at low temperature but are active and stable at high temperature

Antarctic bacterial isolates that produce cold-active extracellular proteases at low temperature but are active and stable at high temperature

Structure and Ecological Roles of a Novel Exopolysaccharide from the Arctic Sea Ice Bacterium Pseudoalteromonas sp. Strain SM20310

The impact of ice melting on bacterioplankton in the Arctic Ocean

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