Extracellular Matrix as a Scaffold for Laryngeal Reconstruction

Abstract

Porcine-derived xenogeneic extracellular matrix (ECM) has been successfully used as a scaffold for tissue repair and reconstruction in numerous preclinical animal studies and human applications. These scaffolds are completely and rapidly degraded and replaced by host-derived tissues that frequently mimic the original tissue composition and architecture. The purpose of the present study was to examine the morphology of ECM scaffolds after their use for laryngeal reconstruction. Thirty adult female dogs were subjected to a partial hemilaryngectomy. The right thyroid cartilage and vocal fold were replaced with ECM scaffold, and the dogs were painlessly sacrificed from 1 week to 12 months after surgery. Histologic examination of the reconstructed tissue showed the presence of a simple squamous epithelial lining, organized glandular structures within the submucosa, reconstructed thyroid cartilage, and bundles of skeletal muscle by 3 months after surgery. We conclude that ECM scaffolds are promising templates for constructive remodeling of laryngeal tissue.

Effect of Porcine Small Intestinal Submucosa on Acute Full‐Thickness Wounds in Dogs

“…Bandage changes and planimetry were performed on days 0, 2,4,6,9,12,15,18,21,24,28,31,35,38,42,45,48,52,55,62, and 69. Biopsies for histopathologic evaluation were taken on days 0, 2, 4, 6, 9, 15, 21, and 31.…”

“…34 There are numerous, mostly experimental, reports on the application of ECM bioscaffolds in the repair and reconstitution of many soft tissue structures with variable success. Tissues include the lower urinary tract, [35][36][37] laryngeal cartilage, 38 esophagus, 39 abdominal wall, [40][41][42][43][44][45] diaphragm, 46,47 dura mater, 48 blood vessels, [49][50][51] tendons, [52][53][54][55][56] ligaments, 57,58 fascia, 59 menisci, [60][61][62][63] and bone. 64 Both commercially available ECM matrices on the veterinary market are promoted as implantable products to accelerate wound healing in dogs and cats.…”

Reprint of: Extracellular matrix as a biological scaffold material: Structure and function

“…Although constructive in vivo remodeling has been observed with the use of hydrated, lyophilized and multilaminated forms of ECM scaffolds [65][66][67][68][69][70][71], ultrastructural changes that occur as a result of dehydration can affect cell attachment, in vivo degradation rate and cellular infiltration [55]. The optimal configuration and method of processing of an ECM scaffold should be determined for each clinical application.…”

The Possibilities of Cell Technologies in the Treatment of Cicatricial Lesions of the Vocal Folds

“…Huber и соавт. предложена методика регенерации голосовых складок с помощью имплантируемого каркаса внеклеточного матрикса свиньи [62]. Перспективным направлением исследований является изучение гелей и гидрогелей в качестве каркасного материала для инъек-ций в голосовые складки, так как они имеют большой по-тенциал для регенерации голосовых связок и улучшения вязкоупругих свойств.…”