Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding

Prewitz, Marina; Stißel, Aline; Friedrichs, Jens; Träber, Nicole; Vogler, Steffen; Bornhäuser, Martin; Werner, Carsten

doi:10.1016/j.biomaterials.2015.09.014

Cited by 79 publications

(65 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparable ECMs derived from human MSCs covalently bound to glass slices via poly-octadecene-alt-maleic anhydride and human fibronectin coating were demonstrated to have an average height of 1 µm. Compliance of these scaffolds was tested to be approximately 100 Pa 20 , which represents BM stroma properties 34 . Only 20% of PB-HSPCs were capable to primarily adhere to the standardized ECM preparations, which might reflect an induced loss of adhesion molecules due to G-CSF mobilization 40, 41 .…”

Section: Discussionmentioning

confidence: 99%

“…Such scaffolds were found to be highly reproducible and provide >500 proteins including major niche proteins like collagens, fibronectin, glycosaminoglycans as well as osteopontin and signaling molecules like stromal derived factor 1 (SDF-1). Thus, adhesive sides and physical cues as they are present in the BM niche 20 are provided. Biomechanical forces of the surrounding environment were found to be transduced to cells via integrin signaling 21 and were demonstrated to control HSPC fate in vitro 22–24 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Kräter

Jacobi

Otto

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, the mechanisms underlying matrix communication and signal transduction are less well understood. Contrary, stem cell culture is mainly facilitated in suspension cultures. Here, we used bone marrow-mimetic decellularized extracellular matrix (ECM) scaffolds derived from mesenchymal stromal cells (MSCs) to study HSPC-ECM interaction. Seeding freshly isolated HSPCs adherent (AT) and non-adherent (SN) cells were found. We detected enhanced expansion and active migration of AT-cells mediated by ECM incorporated stromal derived factor one. Probing cell mechanics, AT-cells displayed naïve cell deformation compared to SN-cells indicating physical recognition of ECM material properties by focal adhesion. Integrin αIIb (CD41), αV (CD51) and β3 (CD61) were found to be induced. Signaling focal contacts via ITGβ3 were identified to facilitate cell adhesion, migration and mediate ECM-physical cues to modulate HSPC function.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Kräter

Jacobi

Otto

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The principle itself of using the secretome as a quality index of biomaterials may be questioned; many proteins reported here are considered part of the extracellular matrix, such as collagens, COMP, SPRC, proteoglycans, FMOD, MATN3 and some of them turn out to be the most distinctive markers of chondrogenesis in our experiments. Indeed, the deposition of proteins into the extracellular matrix in vitro is not an extremely effective process and improving it may be an important aspect of future developments in tissue engineering (Prewitz et al, ). Several other proteins reported here are intrinsically soluble, such as Tissue‐Inhibitor of Metalloproteinases 1, Matrix MetalloProteinase 2, Insulin‐Binding Proteins and connective tissue growth factor.…”

Section: Discussionmentioning

confidence: 99%

Label‐free relative quantification of secreted proteins as a non‐invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair

Henrionnet

Gillet

Mainard

et al. 2017

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Cartilage tissue engineering is making progress, but the competing available strategies still leave room for improvement and consensual overviews regarding the best combinations of scaffolds and cell sources are limited by the capacity to compare them directly. In addition, because most strategies involve autologous cell transfer, once these are optimized, the resulting implants require individual quality control prior to grafting in order to emphasize patient-to-patient differential responsiveness to engineering processes. Here, cartilage substitutes prepared from human mesenchymal stem cells undergoing chondrogenic differentiation within distinct scaffolds were used as pilot samples to investigate the pertinence of a novel method with the aim of characterizing the implants. The limits and advantages of analysing, by label-free liquid chromatography-coupled matrix-assisted laser desorption and ionization (LC-MALDI) mass spectrometry, the secreted proteome released into culture medium by engineered cartilage tissues were investigated and compared with more classically used methods for biomaterial characterization. This method did not require sacrificing the biomaterials and robustly evidenced their chondrogenic statuses. In more detail, the method highlighted differences between batches prepared from distinct donors. It was adapted to distinct scaffolds and allowed a comparison of the influence of individual engineering steps, such as growth factor combinations and oxygen tension. Finally, it evidenced subtle changes between replicate substitutes within a series, thereby distinguishing the least and most accomplished ones. We conclude that relative quantification of secreted proteins through label-free LC-MALDI will be useful, not only to orientate engineering methodologies, but also to ultimately provide non-invasive quality control of engineered tissue substitutes for the repair of cartilage and possibly other connective tissues.

show abstract

“…A recent study employing MSC-secreted extracellular matrix (ECM) as a surface for HSC cultures demonstrates a new way of mimicking the stem cell niche in vitro [80]. Here, the researchers did not create the material interface themselves but employed niche cells to secrete matrix materials.…”

Section: Niche-mimicking Platforms For Modulating Stem Cell Behaviorsmentioning

confidence: 99%

Challenges and Opportunities to Harnessing the (Hematopoietic) Stem Cell Niche

Choi

Harley

2016

Curr Stem Cell Rep

View full text Add to dashboard Cite

In our body, stem cells reside in a microenvironment termed the niche. While the exact composition and therefore the level of complexity of a stem cell niche can vary significantly tissue-to-tissue, the stem cell niche microenvironment is dynamic, typically containing spatial and temporal variations in both cellular, extracellular matrix, and biomolecular components. This complex flow of secreted or bound biomolecules, cytokines, extracellular matrix components, and cellular constituents all contribute to the regulation of stem cell fate specification events, making engineering approaches at the nano- and micro-scale of particular interest for creating an artificial niche environment in vitro. Recent advances in fabrication approaches have enabled biomedical researchers to capture and recreate the complexity of stem cell niche microenvironments in vitro. Such engineered platforms show promise as a means to enhance our understanding of the mechanisms underlying niche-mediated stem cell regulation as well as offer opportunities to precisely control stem cell expansion and differentiation events for clinical applications. While these principles generally apply to all adult stem cells and niches, in this review, we focus on recent developments in engineering synthetic niche microenvironments for one of the best-characterized stem cell populations, hematopoietic stem cells (HSC). Specifically, we highlight recent advances in platforms designed to facilitate the extrinsic control of HSC fate decisions.

show abstract

Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding

Cited by 79 publications

References 39 publications

Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Label‐free relative quantification of secreted proteins as a non‐invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair

Challenges and Opportunities to Harnessing the (Hematopoietic) Stem Cell Niche

Contact Info

Product

Resources

About