Extracellular matrix in healing CO₂ laser incision wound

Abstract

Extracellular matrix components laminin, Type IV collagen, Type III collagen, and fibronectin, of laser‐treated rat tongue mucosa were studied over a healing period of 28 days by using immunohistochemical and electron microscopic techniques. Their distribution and amount in laser wounds was compared with that in scalpel incisions and normal tongue mucosa. Laser treatment caused an extensive destruction of both epithelial and stromal cells but left much of the connective tissue matrix intact. Basement membranes, in particular, appeared to resist laser irradiation. During the study period the laser‐induced lesions healed through reparative synthesis of matrix proteins which led to filling of the tissue defects. The regenerative processes with concomitant re‐epithelialization took place more slowly in laser‐treated than in scalpel incision wounds. The relative resistance of the matrix proteins against laser irradiation and the slow removal and replacement of the residual matrix is suggested to account at least partially for the lack of scarring and contraction frequently observed in laser‐treated areas.

Excision of Mucocele Using Diode Laser in Lower Lip

“…Matrix proteins initiate reparative synthesis on these tissues. Resistance of matrix proteins against laser application and replacement as well as removal of residual matrix is responsible for reduced scarring and contraction [16]. …”

Histological and clinical evaluation of gingival healing following gingivectomy using different treatment modalities

“…The relative resistance of matrix proteins against laser irradiation and the slow removal and replacement of the residual matrix accounts for the lack of scarring and contraction observed in laser-treated areas. [10] Laser-treated sites also have improved adenosine triphosphate synthesis, fibroblast proliferation; collagen synthesis, phagocytosis of macrophages, and acceleration of the inflammatory phase of wound healing. All these mechanisms can result in cellular proliferation and acceleration of the wound healing process.…”

Histological changes in elastic components of soft palate scars after CO2 and contact Nd:YAG laser incisions in the dog as an experimental model

“…This was due to the rapid disintegration of cell structures after CO2 laser evaporization, leaving the extracellular matrix components intact [6]. The residual connective tissue matrix acted as a splint resulting in lack of orientation of proliferating fibroblasts and hindering their getting into close contact with each other.…”