Extracellular Matrix Revisited: Roles in Tissue Engineering

Kim, Youhwan; Ko, Hyojin; Kwon, Ik Keun; Shin, Kwanwoo

doi:10.5213/inj.1620err.001

Cited by 23 publications

(23 citation statements)

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“…Seeded pancreatic cells displayed an affinity towards all modified surfaces of the porous cellulose scaffold that was apparent by formation of structures that resemble lamellipodia and production of a film of ECM. In addition, cells were able to form larger aggregates over 5–10 days, consistent with survival and proliferation seen in other studies (Kim, Kwon, & Shin, 2016; Salvatori et al, 2014). Between Days 1 and 10, sustained culture on the A scaffolds resulted in better expansion, based on a greater distribution of cell clusters throughout the scaffold.…”

Section: Discussionsupporting

confidence: 89%

Insulin production from hiPSC‐derived pancreatic cells in a novel wicking matrix bioreactor

Amini

Paluh

Xie

et al. 2020

Biotech & Bioengineering

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Clinical use of pancreatic β islets for regenerative medicine applications requires mass production of functional cells. Current technologies are insufficient for large‐scale production in a cost‐efficient manner. Here, we evaluate advantages of a porous cellulose scaffold and demonstrate scale‐up to a wicking matrix bioreactor as a platform for culture of human endocrine cells. Scaffold modifications were evaluated in a multiwell platform to find the optimum surface condition for pancreatic cell expansion followed by bioreactor culture to confirm suitability. Preceding scale‐up, cell morphology, viability, and proliferation of primary pancreatic cells were evaluated. Two optimal surface modifications were chosen and evaluated further for insulin secretion, cell morphology, and viable cell density for human‐induced pluripotent stem cell‐derived pancreatic cells at different stages of differentiation. Scale‐up was accomplished with uncoated, amine‐modified cellulose in a miniature bioreactor, and insulin secretion and cell metabolic profiles were determined for 13 days. We achieved 10‐fold cell expansion in the bioreactor along with a significant increase in insulin secretion compared with cultures on tissue culture plastic. Our findings define a new method for expansion of pancreatic cells a on wicking matrix cellulose platform to advance cell therapy biomanufacturing for diabetes.

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Section: Discussionsupporting

confidence: 89%

Insulin production from hiPSC‐derived pancreatic cells in a novel wicking matrix bioreactor

Amini

Paluh

Xie

et al. 2020

Biotech & Bioengineering

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“…Tissue engineering is based on the implantation of biocompatible and biodegradable scaffolds that provides structural support to cell growth and tissue development. The goal is to mimic the microenvironment architecture, mainly represented by the extracellular matrix (ECM), a complex network composed by water and strongly interconnected fibrous/non-fibrous proteins and polysaccharides, that supports tissue growth and mechanical stability and allows optimal homeostasis (Kim et al, 2016). Its biological composition and structural properties are tissue-specific, due to biochemical signals released by different components during tissue morphogenesis and remodeling.…”

Section: Introductionmentioning

confidence: 99%

Investigations of Processing–Induced Structural Changes in Horse Type-I Collagen at Sub and Supramolecular Levels

Terzi

Gallo

Bettini

et al. 2019

Front. Bioeng. Biotechnol.

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The aim of this work is to evaluate the effects of different extraction and material processing protocols on the collagen structure and hierarchical organization of equine tendons. Wide and Small Angle X-ray Scattering investigations on raw powders and thin films revealed that not only the extraction and purification treatments, but also the processing conditions may affect the extent of the protein crystalline domain and induce a nanoscale “shield effect.” This is due to the supramolecular fiber organization, which protects the atomic scale structure from the modifications that occur during fabrication protocols. Moreover, X-ray analyses and Fourier Transform Infrared spectroscopy performed on the biomaterial sheds light on the relationship between processing conditions, triple helical content and the organization in atomic and nanoscale domains. It was found that the mechanical homogenization of the slurry in acidic solution is a treatment that ensures a high content of super-organization of collagen into triple helices and a lower crystalline domain in the material. Finally, mechanical tensile tests were carried out, proving that the acidic solution is the condition which most enhances both mechanical stiffness and supramolecular fiber organization of the films.

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“…The ECM components, mainly proteoglycans and protein, are arranged as nano/microfibers (50–500 nm diameter), allowing the formation of highly interconnected porous network with the adequate structural resilience for specific cellular function [ 4 , 5 ]. Recently, the size and topographical features of ECM structural elements have been identified as a key characteristic that can direct cell behavior [ 2 , 6 ]. In this context, not only the biomaterial composition but also the processing technology plays a key role in the realization of scaffolds with a high degree of biomimicry.…”

Section: Introductionmentioning

confidence: 99%

Influence of Nanofiber Orientation on Morphological and Mechanical Properties of Electrospun Chitosan Mats

Nitti

Gallo

Natta

et al. 2018

Journal of Healthcare Engineering

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This work explored the use of chitosan (Cs) and poly(ethylene oxide) (PEO) blends for the fabrication of electrospun fiber-orientated meshes potentially suitable for engineering fiber-reinforced soft tissues such as tendons, ligaments, or meniscus. To mimic the fiber alignment present in native tissue, the CS/PEO blend solution was electrospun using a traditional static plate, a rotating drum collector, and a rotating disk collector to get, respectively, random, parallel, circumferential-oriented fibers. The effects of the different orientations (parallel or circumferential) and high-speed rotating collector influenced fiber morphology, leading to a reduction in nanofiber diameters and an improvement in mechanical properties.

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Extracellular Matrix Revisited: Roles in Tissue Engineering

Cited by 23 publications

References 0 publications

Insulin production from hiPSC‐derived pancreatic cells in a novel wicking matrix bioreactor

Insulin production from hiPSC‐derived pancreatic cells in a novel wicking matrix bioreactor

Investigations of Processing–Induced Structural Changes in Horse Type-I Collagen at Sub and Supramolecular Levels

Influence of Nanofiber Orientation on Morphological and Mechanical Properties of Electrospun Chitosan Mats

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