Extracellular melibiose and fructose are intermediates in raffinose catabolism during fermentation to ethanol by engineered enteric bacteria

Moniruzzaman, Mohammed; Lai, Xiaokuang; York, Sean W.; Ingram, L. O.

doi:10.1128/jb.179.6.1880-1886.1997

Cited by 20 publications

(16 citation statements)

References 26 publications

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“…Complex nutrients are absent in MM and the rise in pH observed in this medium may be explained by cell lysis and release organic macromolecules that can be utilized by growing cells. The results obtained in this work for KO11 are similar to those observed in a previous study (24), in which greater than 90% of the maximum theoretical yield was reached upon fermentation of 9% sucrose. However, P2 presented poorer results than in that study, producing 73% of the maximum theoretical yield with 8% sucrose and 58% with 12% sucrose.…”

Section: Discussionsupporting

confidence: 91%

Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain ko11 and Klebsiella oxytoca strain P2

Silva¹,

Araújo²,

Silva³

et al. 2005

Braz. J. Microbiol.

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Escherichia coli KO11 and Klebsiella oxytoca P2 recombinants fermented sucrose to ethanol. In minimal medium with 2% or 12% added sucrose KO11 produced 75% and 41%, respectively, of the maximum theoretical yield (0.54g ethanol/g sucrose). In Luria-Bertani (LB) broth with up to 8% sucrose, KO11 presented a 94-96% yield and with 12% sucrose, KO11 presented about 69% yield (44.5g ethanol/L). P2 presented 55% of the theoretical maximum yield in minimal medium supplemented with 2% sucrose and 47% of the maximum in 12% sucrose. In LB broth with 2 or 4% sucrose, P2 presented 94-95% of the theoretical maximum yield, which fell to 73% with 8% added sucrose (31.4g ethanol/L) and 58% with 12% sucrose (37.5 g/L). Volumetric productivity in LB broth containing 2% sucrose was 0.41 g/L/h for KO11 and 1.1 g/L/h for P2, while in LB broth with 12% added sucrose, productivity was 0.96 g/L/h (KO11) and 1.4 g/L/h (P2). During fermentation of sugar cane juice by E. coli KO11 and K. oxytoca P2 produced 39.4 g/L and 42.1 g/L ethanol when supplemented with 0.5% yeast extract, micronutrients and thiamine. In sugar cane juice supplemented with LB broth ingredients, KO11 ethanol fermentation was inhibited with production of only 23.0 g ethanol/L, while P2 produced 44.2 g/ L. Ethanol production by KO11 and P2, respectively, in sugarcane juice was a) 25.3 and 30.2 g/L with 0.2% ammonium sulfate, b) 24.9 and 31.6 g/L with ammonium sulfate and micronutrients, c) 25.6 and 37.5 g/L with ammonium sulfate, micronutrients and thiamine. During molasses fermentation E. coli KO11 presented low ethanol production and high lactic acid production. K. oxytoca P2 produced 25 g ethanol/L in molasses diluted 10-fold in water, with or without addition of 0.5% yeast extract, and 27.8 g/L with addition of LB broth ingredients after 96h. P2 produced 24.5, 26.9, and 28.0 g ethanol/L in molasses diluted 10-fold in vinasse, vinasse with 0.5% added yeast extract and with LB broth ingredients, respectively.

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Section: Discussionsupporting

confidence: 91%

Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain ko11 and Klebsiella oxytoca strain P2

Silva¹,

Araújo²,

Silva³

et al. 2005

Braz. J. Microbiol.

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“…They found that the hydrolysis efficiencies of raffinose and melibiose after 120 h were below 25% and 50% in the media that contained 50 g dm −3 and 20 g dm −3 of these sugars, respectively. In another study, Moniruzzaman et al 12 found that the hydrolysis efficiencies of raffinose after 24 h with E chrysanthemi, K oxytoca and E coli were 73%, 69% and 43%, respectively, in the media that contained this sugar in a concentration of 90 g dm −3 . However, raffinose and produced melibiose were completely hydrolyzed by E chrysanthemi and K oxytoca after 144 h from the beginning of the process, and more than 83% of the produced galactose accumulated in the fermentation media.…”

Section: Discussionmentioning

confidence: 97%

“…These facts were taken into consideration for a development of recombinant strains of baker's yeasts that produce α-galactosidase. 7 -10 Recombinant bacteria strains that ferment raffinose, such as Zymomonas mobilis, 11 Escherichia coli, Klebsiella oxytoca and Erwinia chrysanthemi 12 were developed for ethanol production. However, raffinose was not completely utilized and low ethanol yields were obtained with these microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Utilization of raffinose and melibiose by a mutant of Saccharomyces cerevisiae

Atiyeh¹

2003

J of Chemical Tech & Biotech

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The mutant Saccharomyces cerevisiae ATCC 36858 was used in the production of ethanol and/or fructose from synthetic media in batch processes with raffinose, melibiose or sucrose. The mutant was able to hydrolyze all the sugars used and to selectively ferment glucose and galactose to ethanol while fructose accumulated in the fermentation medium. The fructose yield was above 89% of the theoretical value in the media with either raffinose or sucrose, when initial concentrations were between 131.5 g dm −3and 242.0 g dm −3 . The ethanol yields were 82% and 77% of the theoretical values in the media with melibiose and sucrose, respectively, and about 72% of the theoretical value when raffinose was used. The fructose fraction in the carbohydrate content of the produced syrups was more than 96% when raffinose concentration was below 189.1 g dm −3 . However, even at a sucrose concentration of 187.9 g dm −3 , the produced syrup contained 100% fructose. Some oligosaccharides were also produced in all tested media. The produced oligosaccharides were consumed by the end of the fermentation process. These findings can be useful in the production of ethanol and high fructose syrups using raw materials based on sucrose and raffinose such as molasses.

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“…Cornelis et al (1978) reported that raffinose can be transported by the lactose permease, and isolated lacI constitutive mutants of E. coli K-12 capable of raffinose uptake and metabolism. Moniruzzaman et al (1997) isolated raffinose positive mutants of KO11, a derivative of E. coli B, by culture enrichment in presence of raffinose and the adapted strain may contain an analogous mutation.…”

Section: Fermentation Of Galactose Sucrose Raffinose and Stachyosementioning

confidence: 99%

Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions

Thakker¹,

San²,

Bennett³

2013

Bioresource Technology

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E. coli strains HL2765 and HL27659k harboring pRU600 and pKK313 were examined for succinate production under aerobic conditions using galactose, sucrose, raffinose, stachyose, and mixtures of these sugars extracted from soybean meal and soy solubles. HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 87 mM and 98 mM hexose of soybean meal extract and produced 83 mM and 95 mM succinate, respectively. While using soy solubles extract, HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 160 mM and 187 mM hexose and produced 158 mM and 183 mM succinate, respectively. Succinate yield of HL2765(pKK313)(pRU600) was low as compared to that of HL27659k(pKK313)(pRU600) while using acid hydrolysate of soybean meal or soy solubles extracts. Maximum succinate production of 312 mM with a molar yield of 0.82 mol/mol hexose was obtained using soy solubles hydrolysate by HL27659k(pKK313)(pRU600). This study demonstrated the use of soluble carbohydrates of the renewable feedstock, soybean as an inexpensive carbon source to produce succinate by fermentation.

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Extracellular melibiose and fructose are intermediates in raffinose catabolism during fermentation to ethanol by engineered enteric bacteria

Cited by 20 publications

References 26 publications

Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain ko11 and Klebsiella oxytoca strain P2

Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain ko11 and Klebsiella oxytoca strain P2

Utilization of raffinose and melibiose by a mutant of Saccharomyces cerevisiae

Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions

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