Extracellular probiotic lipase capped silver nanoparticles as highly efficient broad spectrum antimicrobial agents

Khan, Imran; Sivasankaran, Nivetha; Nagarjuna, Ravikiran; Ganesan, Ramakrishnan; Dutta, Jayati Ray

doi:10.1039/c8ra05999c

Cited by 16 publications

(6 citation statements)

References 51 publications

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“…The gentle shift in the amide stretching peak changed from 1640 to 1627 cm −1 . It could be attributed to the electrostatic interaction between the surface of AgNPs and the amide bonds of proteins, which was in line with the previous literature [ 14 ].…”

Section: Resultssupporting

confidence: 91%

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Pan

Tao

et al. 2021

Molecules

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The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.

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Section: Resultssupporting

confidence: 91%

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Pan

Tao

et al. 2021

Molecules

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“…[34] Among several candidates, ionic silver stands out the best due to its high bactericidal activity and low propensity to develop resistance with repeated exposure. [35][36][37][38][39][40][41] However, most of the employed approaches have incorporated ionic silver or silver nanoparticles throughout the dressing matrix− a strategy that compromises the availability of silver at the surface, which remains in actual contact with the wound. [42,43] On a different note, quaternary ammonium moieties are wellknown for their antibacterial activity via membrane disruption and depolarization.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Facile Surface Derivatization of Poly(ε‐caprolactone)‐Based Wound Dressing Materials Imparting Anti‐Infective, Excessive Biofluid Drainage, and Easy‐to‐Peel Characteristics

Mude

Tata

Trinath

et al. 2023

Advanced Therapeutics

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The rapid emergence of antimicrobial resistance warrants an antibiotic‐free approach to counter the bacterial threat in all possible applications of environmental and biomedical domains. In the context of smart wound dressing, besides imparting anti‐infective characteristics in a nonconventional fashion, it is also essential to imbibe multifunctional attributes like excessive biofluid drainage, easy‐to‐peel, optimal gas permeation, etc., for which appropriate material design is a prime requisite. In this work, poly(ε‐caprolactone) (PCL)− an FDA approved biocompatible polyester− is chosen for imparting antimicrobial properties by surface derivatization with molecularly‐dispersed ionic silver over quaternary ammonium moieties through a facile room temperate and organic solvent‐free approach. To render the other characteristics mentioned above, two different systems comprising PCL with varying porosity, namely, electrospun fibers and polyester‐coated cotton gauze, are developed. The dressing materials are thoroughly characterized and assessed for their structural, surface chemical, morphological, wettability, antibacterial, and peel strength properties as a function of surface derivatization steps. The bactericidal performance of the quaternary ammonium‐functionalized surfaces has enhanced manifold (≈7–10) after derivatizing with ionic silver. The in vivo efficacy study employing the dressing materials reveals ionic silver derivatized electrospun fibers as the most promising candidate, followed by cotton gauze‐coated with 5% PCL.

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“…Traditionally, silver-based substances such as Ag nanoparticles, AgBr, etc., have been used for antimicrobial applications, as they do not easily develop antimicrobial resistance through genetic mutations. − In recent years, photocatalytic disinfection using noble metal ion (Ag and Au)-doped semiconducting oxides such as TiO 2 , ZnO, etc., have been shown to possess strong antimicrobial properties . However, many of these materials work well in the ultraviolet region rather than the visible light region of the electromagnetic spectrum.…”

Section: Introductionmentioning

confidence: 99%

Highly Dispersed Nanocomposite of AgBr in g-C₃N₄Matrix Exhibiting Efficient Antibacterial Effect on Drought-ResistantPseudomonas putidaunder Dark and Light Conditions

Balapure

Nikhariya

Boppudi

et al. 2020

ACS Appl. Mater. Interfaces

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Synthesis of nanocomposites possessing intimately mixed components is highly challenging to bring out the best possible properties of the materials. The challenge is mainly due to the difficulties associated with controlling the phase segregation of individual components as a result of high interfacial tension between them and cohesive forces within each component during the synthesis. Here, we show a single-step synthesis of representative nanocomposites of g-C 3 N 4 /AgBr through a rationally designed approach, wherein melamine, the precursor of g-C 3 N 4 , has been intimately mixed with the AgBr precursor, silver− tetraoctylammonium bromide. Subsequent calcination of the obtained solid at 500 °C has resulted in the formation of highly dispersed g-C 3 N 4 /AgBr. The key to such a high dispersion lies in the surfactant-based AgBr precursor that minimized the interfacial tension during the process. The AgBr content has been varied between 2 and 20 wt % with respect to the g-C 3 N 4 content. The obtained nanocomposites have been thoroughly characterized using XRD, XPS, ED-XRF, FE-SEM, HR-TEM, DRS, TCSPC, and BET surface area techniques. The studies revealed a high dispersion of AgBr in the g-C 3 N 4 matrix. The nanocomposites have been found to exhibit remarkable antimicrobial properties over a droughtresistant bacterial strain of Pseudomonas putida under both dark and light conditions compared with similar compositions obtained through other methods reported so far. The present study offers a new approach for synthesizing highly dispersed and efficient nanocomposites.

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Extracellular probiotic lipase capped silver nanoparticles as highly efficient broad spectrum antimicrobial agents

Abstract: A series of Ag NPs capped with different concentrations of probiotic lipase have been synthesized. The obtained Ag NPs exhibited efficient broad spectrum antimicrobial activity at concentrations much lower than the LC50values of zebrafish.

Cited by 16 publications

References 51 publications

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Fabrication and Facile Surface Derivatization of Poly(ε‐caprolactone)‐Based Wound Dressing Materials Imparting Anti‐Infective, Excessive Biofluid Drainage, and Easy‐to‐Peel Characteristics

Highly Dispersed Nanocomposite of AgBr in g-C₃N₄Matrix Exhibiting Efficient Antibacterial Effect on Drought-ResistantPseudomonas putidaunder Dark and Light Conditions

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Extracellular probiotic lipase capped silver nanoparticles as highly efficient broad spectrum antimicrobial agents

Abstract: A series of Ag NPs capped with different concentrations of probiotic lipase have been synthesized. The obtained Ag NPs exhibited efficient broad spectrum antimicrobial activity at concentrations much lower than the LC50values of zebrafish.

Cited by 16 publications

References 51 publications

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

Fabrication and Facile Surface Derivatization of Poly(ε‐caprolactone)‐Based Wound Dressing Materials Imparting Anti‐Infective, Excessive Biofluid Drainage, and Easy‐to‐Peel Characteristics

Highly Dispersed Nanocomposite of AgBr in g-C3N4Matrix Exhibiting Efficient Antibacterial Effect on Drought-ResistantPseudomonas putidaunder Dark and Light Conditions

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Product

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Highly Dispersed Nanocomposite of AgBr in g-C₃N₄Matrix Exhibiting Efficient Antibacterial Effect on Drought-ResistantPseudomonas putidaunder Dark and Light Conditions