Extracellular recording of glycine receptor chloride channel activity as a prototype for biohybrid sensors

Sommerhage, Frank; Baumann, Arnd; Wrobel, Günter; Ingebrandt, Sven; Offenhäusser, Andreas

doi:10.1016/j.bios.2010.05.031

Cited by 11 publications

(11 citation statements)

References 48 publications

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“…However in this use, the cells grow two-dimensionally and are combined with chiplike scaffolds or matrices for sensing [21,22]. Since these biosensing approaches are applied mainly in vitro, biocompatibility is currently a minor issue.…”

Section: Biohybrid Biosensorsmentioning

confidence: 99%

Designing the Biocompatibility of Biohybrids

Witte

Bartsch

Willbold

2011

Tissue Engineering III: Cell - Surface Interactions for Tissue Culture

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Biohybrid has been used as a fashionable term in scientific publications during the past years to describe a functional unit consisting of a bioactive and a structural component. The bioactive part of the biohybrid could consist of cells or bioactive molecules, while the structural part is of biological or non-biological origin. Biohybrids are currently used as implants and transplants in regenerative medicine or in vitro applications such as assays, biosensors or bioreactors. However, a clear definition of a biohybrid has not been given yet. This chapter reviews the current applications of biohybrids and identifies the challenges of biohybrids in in vivo applications. A classification of biohybrids according to their functional use and application is provided.

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Section: Biohybrid Biosensorsmentioning

confidence: 99%

Designing the Biocompatibility of Biohybrids

Witte

Bartsch

Willbold

2011

Tissue Engineering III: Cell - Surface Interactions for Tissue Culture

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“…At the same time, detecting cells or rat cardiomyocytes confined in a sensing area has also been proposed in previous reports (Baumann et al 1999) (Pui et al 2009). Single-cell detection in a confined sensitive area has also been achieved by using sensors such as the microelectrode array (Chen et al 2003) (Gray et al 2004) (Revzin et al 2004), the open-gate field-effect transistor (Baumann et al 1999) (Schäfer et al 2009) (Sommerhage et al 2010) arrays, and SiNW transistor arrays (Patolsky et al 2006) (Karni et al 2009). In this work, we used moldcast PDMS for the confined sensitive area (isolation window).…”

Section: Introductionmentioning

confidence: 98%

“…It has been proven that, compared to normal tissue, tumors require a high level of glucose so as to consistently acidify their environment in order to support the metabolism, resulting in a lower extracellular pH value. Since a PSW sensor has been proven to be able to detect the H + ion density (pH value) of the medium coated on the PSW surface (Hsu et al 2009) (Wu et al 2011) and different cells produce different extracellular acidification and hence different H + ion densities (Baumann et al 1999) (Schäfer et al 2009) (Sommerhage et al 2010), it is therefore expected that any change in the extracellular microenvironment of the single cell confined in the isolation window residing on the PSW will alter the surface-charge state of the PSW and can therefore be detected by the change in the current flowing through the PSW channel. In this paper, we report for the first time the differences in extracellular cell property between normal cells and cancer cells by using a PSW in combination with an isolation window.…”

Section: Introductionmentioning

confidence: 99%

Electrical characterization of single cells using polysilicon wire ion sensor in an isolation window

Hsu

et al. 2011

Biomed Microdevices

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A polysilicon wire (PSW) sensor can detect the H(+) ion density (pH value) of the medium coated on its surface, and different cells produce different extracellular acidification and hence different H(+) ion densities. Based on this, we used a PSW sensor in combination with a mold-cast polydimethylsiloxane (PDMS) isolation window to detect the adhesion, apoptosis and extracellular acidification of single normal cells and single cancer cells. Single living human normal cells WI38, MRC5, and BEAS-2B as well as non-small-cell lung cancer (NSCLC) cells A549, H1299, and CH27 were cultivated separately inside the isolation window. The current flowing through the PSW channel was measured. From the PSW channel current change as a function of time, we determined the cell adhesion time by observing the time required for the current change to saturate, since a stable extracellular ion density was established after the cells were completely adhered to the PSW surface. The apoptosis of cells can also be determined when the channel current change drops to zero. We found that all the NSCLC cells had a higher channel current change and hence a lower pH value than the normal cells anytime after they were seeded. The corresponding average pH values were 5.86 for A549, 6.00 for H1299, 6.20 for CH27, 6.90 for BEAS-2B, 6.96for MRC5, and 7.02 for WI38, respectively, after the cells were completely adhered to the PSW surface. Our results show that NSCLC cells have a stronger cell-substrate adhesion and a higher extracellular acidification rate than normal cells.

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“…Image 1 shows the structure of the microfluidic system. The input (1) and the first membrane pump for the distribution of the medium and other fluids (2) are located at the right hand side. The fluid contained in the measuring chamber (6) at the left side is circulated by the internal micro pump (5).…”

Section: Chip Systemmentioning

confidence: 99%

“…The significant advantage of whole cell assays in comparison to biomolecules results from the direct access to hardly obtainable parameters such as toxicity, mutagenicity and pharmacological effectiveness. The main fields of application of cell based assays can be found in pharmaco-and toxicokinetics [2], biomedicine [3], environmental monitoring [4] as well as in basic research such as cell physiology and stem cell sciences. Among cell based assays, reporter gene assays became more and more important, because they are very flexible in varying the properties of the biological component by genetic modification.…”

Section: Introductionmentioning

confidence: 99%

Automated universal chip platform for fluorescence based cellular assays

Schmieder

Eger

et al. 2012

Biomedical Engineering / Biomedizinische Technik

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The advantage of cell based assays used as biosensors is the direct access to hardly obtainable parameters like toxicity, mutagenicity and pharmacological effectiveness. Within the last few years we established a micro fluidic platform including a peristaltic micro pump as well as several valves, manifolds and micro channels [1]. For optical online monitoring the micro fluidic system is bonded to a glass slide. Furthermore the biochip is fixed on an electrically heated support. The pneumatically actuated peristaltic pump as well as the temperature control is performed by a control device. For the fluorescence based online monitoring a robotic guided fluorescence measurement module was developed, which supports the detection of fluorescence in microtiter plates and microfluidic systems. This measurement module allows the fluorescence detection of two different excitation / detection wavelengths (480 / 530 nm and 570 / 620 nm) and was successfully characterised using EGFP and Rhodamine 6G. Additionally three cell based assays with bacterial, yeast and human cells were characterized.

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Extracellular recording of glycine receptor chloride channel activity as a prototype for biohybrid sensors

Cited by 11 publications

References 48 publications

Designing the Biocompatibility of Biohybrids

Designing the Biocompatibility of Biohybrids

Electrical characterization of single cells using polysilicon wire ion sensor in an isolation window

Automated universal chip platform for fluorescence based cellular assays

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