Extracellular <scp>DNA</scp> builds and interacts with vibrio polysaccharide in the biofilm matrix formed by <i>Vibrio cholerae</i>

Kanampalliwar, Amol; Singh, D. V.

doi:10.1111/1758-2229.12870

Cited by 14 publications

(7 citation statements)

References 79 publications

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“…T6SS-mediated eDNA release has previously been shown to facilitate horizontal gene transfer in V. cholerae grown on chitin, implying that strain interfaces where T6SS killing occurs have the potential to be evolutionary hotbeds ( 44 , 54 ). eDNA has additionally been shown to interact with VPS in V. cholerae biofilms ( 55 ), suggesting that eDNA release via T6SS could also play a structural role within biofilms.…”

Section: Resultsmentioning

confidence: 99%

VxrB Influences Antagonism within Biofilms by Controlling Competition through Extracellular Matrix Production and Type 6 Secretion

Teschler

Jiménez-Siebert

Jeckel

et al. 2022

mBio

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Section: Resultsmentioning

confidence: 99%

VxrB Influences Antagonism within Biofilms by Controlling Competition through Extracellular Matrix Production and Type 6 Secretion

Teschler

Jiménez-Siebert

Jeckel

et al. 2022

mBio

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“…The biofilm 3D structure of Bacillus subtilis showed that polysaccharides could interconnect with eDNA in the early stage of biofilm formation, and was mainly the structure material of the mature biofilm (Peng et al, 2019). Kanampalliwar and Singh (2020) reported that the interaction between eDNA and polysaccharides could contribute to the biofilm integrity of Vibrio cholerae. In this research, the S-EPS 2 could bond on the anion exchange column DEAE-Sepharose Fast Flow during the S-EPS purified process and S-EPS 2-1 was the main component of S-EPS 2, indicating that S-EPS 2-1 might have the negative charge (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri

Song

Sun

et al. 2022

Front. Microbiol.

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Shigella flexneri is a waterborne and foodborne pathogen that can damage human health. The exopolysaccharides (S-EPS) produced by S. flexneri CMCC51574 were found to promote biofilm formation and virulence. In this research, the crude S-EPS produced by S. flexneri CMCC51574 were separated into three main different fractions, S-EPS 1-1, S-EPS 2-1, and S-EPS 3-1. The structure of the S-ESP 2-1 was identified by FT-IR, ion chromatography analysis, methylation analysis, and NMR analysis. The main chain of S-EPS 2-1 was α-Manp-(1 → 3)-α-Manp-[(1 → 2,6)-α-Manp]15-[(1 → 2)-Manf-(1→]8; there were two branched-chain R1 and R2 with a ratio of 4:1, R1: α-Manp-(1 → 6)- and R2: α-Manp-(1 → 6)- Glc-(1 → 6)- were linked with (1 → 2,6)-α-Manp. It was found that S-EPS 2-1 exhibited the highest promoting effect on biofilm formation of S. flexneri. The S-EPS 2-1 was identified to interact with extracellular DNA (eDNA) of S. flexneri, indicating that the S-EPS 2-1 was the specific polysaccharide in the spatial structure of biofilm formation. Our research found the important role of S-EPS in S. flexneri biofilm formation, which will help us to understand the underlining mechanisms of the biofilm formation and find effective ways to prevent S. flexneri biofilm infection.

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“…In this same sense, the copper (I) complex could affect eDNA, which is an important part of EPS and plays a key role in the initial adhesion and structural stability of the biofilm in Gram-negative and -positive bacteria [44]. Studies carried out in Vibrio cholerae have shown that eDNA is able to interact with the exopolysaccharides in the EPS, improving strength and stability of the biofilm and contributing to antimicrobial resistance [45]. In this respect, in analyses carried out in P. aeruginosa with copper (II) complexes, it has been shown that they are capable of increasing the antibiofilm effect by inhibiting the amount of biofilm eDNA [46].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Copper (I) Complex [Cu(NN1)2](ClO4) on Vibrio harveyi BB170 Biofilm Formation

et al. 2021

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Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN1)2]ClO4, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in Vibrio harveyi BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory concentration (IC50) determination. Antibiofilm capacity of copper (I) complex was analyzed by different concentrations of IC50 values. The results showed that the sub-IC50 concentration, 12.6 µg/mL of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the [Cu(NN1)2]ClO4 complex affected the biofilm structure. Therefore, the copper (I) complex is effective as an antibiofilm compound in V. harveyi BB170.

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Extracellular DNA builds and interacts with vibrio polysaccharide in the biofilm matrix formed by Vibrio cholerae

Cited by 14 publications

References 79 publications

VxrB Influences Antagonism within Biofilms by Controlling Competition through Extracellular Matrix Production and Type 6 Secretion

VxrB Influences Antagonism within Biofilms by Controlling Competition through Extracellular Matrix Production and Type 6 Secretion

The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri

In Vitro Effect of Copper (I) Complex [Cu(NN1)2](ClO4) on Vibrio harveyi BB170 Biofilm Formation

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