Extracellular β-class carbonic anhydrase of the alkaliphilic cyanobacterium Microcoleus chthonoplastes

Kupriyanova, Elena V.; Sinetova, Maria A.; Markelova, A. G.; Allakhverdiev, Suleyman I.; Пронина, Н. А.

doi:10.1016/j.jphotobiol.2011.01.021

Cited by 43 publications

(38 citation statements)

References 32 publications

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“…The CA in carboxysomes converts the accumulated HCO 3 ‐ into CO 2 , providing the substrate for the photosynthetic enzyme ribulose‐d‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCo). The CO 2 is used by RuBisCo to start the initial reaction in the Calvin cycle, the carboxylation of ribulose bisphosphate, while the CA in the periplasmic space prevents the escape of CO 2 from the cell by converting it into HCO 3 ‐ …”

Section: Resultsmentioning

confidence: 99%

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

et al. 2018

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The simultaneous production of carbonic anhydrase and the phycobiliproteins, C-phycocyanin and allophycocyanin, was evaluated during the cultivation of Spirulina platensis using Zarrouk's medium in its non-diluted (100 % ZM) and diluted to 20 % (v/v) (20 % ZM) forms. In both media, Spirulina simultaneously produced carbonic anhydrase and phycobiliproteins. For the phycobiliproteins' production, higher concentrations (mg/g) of C-phycocyanin and allophycocyanin were obtained when 100 % ZM was used. For carbonic anhydrase production, higher concentrations (U/g) were obtained on the 6 th day of cultivation in both culture media. 100 % ZM promoted greater productions of these bioproducts, and, for simultaneous obtainment, the cells should be harvested after 21 days, obtaining concentrations of 137.4 mg/g C-phycocyanin, 104.4 mg/g allophycocyanin, and 26.6 U/g carbonic anhydrase.

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Section: Resultsmentioning

confidence: 99%

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

et al. 2018

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“…Recently it was shown that a truncated CcmM protein indeed formed an active CA, the activity of which is strongly affected by the redox status of the cells [ 48 ]. In addition to the CAs inside the carboxysomes, there are hints of CA activity associated with the periplasm or outer surface of the cyanobacteria, possibly contributing to the cyanobacterial CCM [ 49 ].…”

Section: General Description Of Function and Components Of The Cyamentioning

confidence: 99%

Regulation of CO2 Concentrating Mechanism in Cyanobacteria

Burnap

Hagemann

Kaplan

2015

Life

164

155

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In this chapter, we mainly focus on the acclimation of cyanobacteria to the changing ambient CO2 and discuss mechanisms of inorganic carbon (Ci) uptake, photorespiration, and the regulation among the metabolic fluxes involved in photoautotrophic, photomixotrophic and heterotrophic growth. The structural components for several of the transport and uptake mechanisms are described and the progress towards elucidating their regulation is discussed in the context of studies, which have documented metabolomic changes in response to changes in Ci availability. Genes for several of the transport and uptake mechanisms are regulated by transcriptional regulators that are in the LysR-transcriptional regulator family and are known to act in concert with small molecule effectors, which appear to be well-known metabolites. Signals that trigger changes in gene expression and enzyme activity correspond to specific “regulatory metabolites” whose concentrations depend on the ambient Ci availability. Finally, emerging evidence for an additional layer of regulatory complexity involving small non-coding RNAs is discussed.

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“…The ensuing elevation of the CO 2 /O 2 ratio increases its efficiency as carboxylase (Giordano et al, 2005 ). Extracellular carbonic anhydrase (eCA) has been detected in several phototrophic microorganisms, both in cyanobacteria and in eukaryotes, and is thought to have a role in DIC uptake into the cell (Katsunori and Shigetoh, 1986 ; Nimer et al, 1999 ; Kupriyanova et al, 2003 , 2007 , 2011 ; Karim et al, 2011 ; Hopkinson et al, 2013 ; Hamizah et al, 2017 ). This may be increasingly important at higher pH and/or lower dissolved free CO 2 concentrations (i.e., hyperalkaline environments).…”

Section: Introductionmentioning

confidence: 99%

Role of Extracellular Carbonic Anhydrase in Dissolved Inorganic Carbon Uptake in Alkaliphilic Phototrophic Biofilm

Sharp

Ataeian

et al. 2018

Front. Microbiol.

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Alkaline Soda Lakes are extremely productive ecosystems, due to their high dissolved inorganic carbon (DIC) concentrations. Here, we studied the dynamics of the carbonate system, in particular, the role of extracellular carbonic anhydrase (eCA) of an alkaliphilic phototrophic biofilm composed of bacteria enriched from soda lake benthic mats. By using measurements with microsensors and membrane inlet mass spectrometry, combined with mathematical modeling, we show how eCA controls DIC uptake. In our experiments, the activity of eCA varied four-fold, and was controlled by the bicarbonate concentration during growth: a higher bicarbonate concentration led to lower eCA activity. Inhibition of eCA decreased both the net and the gross photosynthetic productivities of the investigated biofilms. After eCA inhibition, the efflux of carbon dioxide (CO2) from the biofilms increased two- to four-fold. This could be explained by the conversion of CO2, leaking from cyanobacterial cells, by eCA, to bicarbonate. Bicarbonate is then taken up again by the cyanobacteria. In suspensions, eCA reduced the CO2 leakage to the bulk medium from 90 to 50%. In biofilms cultivated at low bicarbonate concentration (~0.13 mM), the oxygen production was reduced by a similar ratio upon eCA inhibition. The role of eCA in intact biofilms was much less significant compared to biomass suspensions, as CO2 loss to the medium is reduced due to mass transfer resistance.

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Extracellular β-class carbonic anhydrase of the alkaliphilic cyanobacterium Microcoleus chthonoplastes

Cited by 43 publications

References 32 publications

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

Regulation of CO2 Concentrating Mechanism in Cyanobacteria

Role of Extracellular Carbonic Anhydrase in Dissolved Inorganic Carbon Uptake in Alkaliphilic Phototrophic Biofilm

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