Extract Corn Silk with Variation of Solvents on Yield, Total Phenolics, Total Flavonoids and Antioxidant Activity

Abstract

Corn silk is a by product that is commonly used as a traditional medicine that contains bioactive compounds such as volatile oils, steroids, alkaloids, tannins, flavonoids, chlorogenic acid and phenolic compounds, containing minerals, high crude fiber, vitamins (B, C, and K), steroids such as sitosterol and stigmasterol, alkaloids, saponins, tannins, flavonoids, anthocyanins, etc. The solvent used for the extraction of flavonoid compounds are methanol, ethanol, acetone and ethyl acetate. Basic separation by solvent extraction is the difference in the solubility of each composition in compaction with solvent. Solubility is influenced temperature, stirring speed, the extraction time, comprehensive tangent plane solids by solvent extraction and frequency. This study aims to extract corn silk by using a variation of the solvent type in terms of yield, total phenols, flavonoids and antioxidant activity. The variations of solvent are methanol: water; ethanol: water; ethyl acetate:water, acetone: water, (85:15, v/v). Variations in the type of solvent with the highest yield at a ratio methanol:water, and the ratio ethanol:water, then the ratio acetone:water and the lowest ratio of ethyl acetate:water. At ratio methanol:water obtained yield is 65.8%, total phenol is 45350.27 mg/kg GAE, total flavonoids is 291.28 mg QE/kg and activities of antioxidant is 92.1%.

Waste to Medicine: Evidence from Computational Studies on the Modulatory Role of Corn Silk on the Therapeutic Targets Implicated in Type 2 Diabetes Mellitus

“…The high abundance of (7 R)-(+)-Lyoniresinol 9 -glucoside, cnicin, methyisocitric acid, chrysoeriol 4 ,7-diglucoronide, and 3-isopropylmalatte in the aqueous extract and D-leucic acid, dodecanedioc acid, quercetin-3-(2 ,3 ,4 -triacetylgalactoside), sebacic acid, and pcoumaroyl malic acid in the ethanolic extract and vice versa could be attributed to the degree of polarity of the extracting medium [55] and may explain or buttress the variation (46.8%) in the types and amounts of phytoconstituents (generally) as identified based on UPLC-MS analysis. While the use of this technique is adequate and reliable, as buttressed in the work of Fougre et al [56], adopting a related tool, the high abundance of phytoconstituents, namely, azealic acid, isowertin 2 -rhamnoside, D-2-hydrozyglutaric acid, citraconic acid, 3-p-courmaroylquinic acid, cis-aconitic acid, UNPD129404, caffeic acid ethyl ester, and myricitrin, particularly in the raw CS sample, may be suggested to contribute to the pharmacological attributes (glucose lowering) of CS, since the concentration of bioactive ingredients within a medicinal sample is well-established to influence the pharmacological effectiveness.…”

Phytochemicals Derived from Agricultural Residues and Their Valuable Properties and Applications

“…Ren et al [ 61 ] successfully isolated and separated compounds such as 2″- O - α - l -rhamnosyl-6-C-3″-deoxyglucosyl-3′-methoxyluteolin, ax-5′-methane-3′-methoxymaysin, ax-4″-OH-3′-methoxymaysin, 6,4′-dihydroxy-3′-methoxyflavone-7- O -glucoside, and 7,4′-dihydroxy-3′-methoxyflavone-2″- O - α - l -rhamnosyl-6-C fucoside from corn silk. Moreover, among flavonoids, Haslina and Eva [ 43 ] determined in corn silk: apigmaysin, maysin, isoorientin-2″- O - α - l -rhamnoside, 3-methoxymaysine, and ax-4-OH maysin.…”

The Effect of Ratio of Methanol and Concentration of Methanol in Corn Silk Extracts with Ultrasonic-assisted Extraction

“…[12], extract the antioxidant components of corn silk using methanol and water, with the total polyphenol content in the methanol and water extracts being 272.81 mgGAE/100g and 256.36 mgGAE/100 mg, respectively (dry). The extract of sweet corn silk with the solvent combination (methanol: water) (85:15) was higher than the extract with the solvent combinations (ethanol: water), (acetone: water), and (ethyl acetate: water) [13]. The research that was conducted in the extract of soursop leaves best using the ultrasonic method of long extraction 20 minutes [14].…”