Extracting fluorescence efficiency and emission spectra of cervical tissue

Tu, Shih-Cheng; Sung, Kung-Bin

doi:10.1364/fio.2018.jtu3a.107

Cited by 1 publication

(1 citation statement)

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“…When FLIM images of the tissues or cells were collected with a 447 ± 30 nm band-pass filter for NAD(P)H, the signals might partially have the components of elastin and collagen. However, NAD(P)H predominantly exists in the epithelium and collagen mainly exists in the stroma [41], and in this work, the samples were imaged and analyzed at cervical epithelial area, thus the main fluorophore here is NAD(P)H, while extracellular matrix components (collagens type I and III and elastin) play a less relevant role, as reported by previous cervical tissue fluorescence spectra studies [42][43][44].…”

Section: Flim Experimental Setupmentioning

confidence: 82%

Discriminating different grades of cervical intraepithelial neoplasia based on label-free phasor fluorescence lifetime imaging microscopy

Wang¹,

Wang

Zhang³

et al. 2020

Biomed. Opt. Express

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This study proposed label-free fluorescence lifetime imaging and phasor analysis methods to discriminate different grades of cervical intraepithelial neoplasia (CIN). The human cervical tissue lesions associated with cellular metabolic abnormalities were detected by the status changes of important coenzymes in cells and tissues, reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD). Fluorescence lifetime imaging microscopy (FLIM) was used to study human cervical tissues, human cervical epithelial cells, and standard samples. Phasor analysis was applied to reveal the interrelation between the metabolic changes and cancer development, which can distinguish among different stages of cervical lesions from low risk to high risk. This approach also possessed high sensitivity, especially for healthy sites of CIN3 tissues, and indicated the dominance of the glycolytic pathway over oxidative phosphorylation in high-grade cervical lesions. This highly adaptive, sensitive, and rapid diagnostic tool exhibits a great potential for cervical precancer diagnosis.

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