Extracting structural insights from soft X-ray scattering of biological assemblies

Rongpipi, Sintu; Mundo, Joshua T. Del; Gomez, Enrique D.; Gomez, Esther W.

doi:10.1016/bs.mie.2022.09.017

Cited by 1 publication

(2 citation statements)

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“…X-ray scattering is a nondestructive analytical method that reveals the microstructure of materials . Furthermore, using resonant X-ray Scattering leverages compositional differences in a given material by tuning incident X-ray energy within the soft (0.2 to 2 keV), tender (2 to 8 keV), or hard (>8 keV) X-ray regime at the absorption edges of specific elements .…”

Section: Introductionmentioning

confidence: 99%

“…X-ray scattering is a nondestructive analytical method that reveals the microstructure of materials. 11 Furthermore, using resonant X-ray Scattering leverages compositional differences in a given material by tuning incident X-ray energy within the soft (0.2 to 2 keV), tender (2 to 8 keV), or hard (>8 keV) X-ray regime at the absorption edges of specific elements. 3 Pectic HG differs from cellulose and hemicellulose in that it is negatively charged, and de-esterified galacturonic acid residues can be cross-linked by calcium ions (Ca 2+ ).…”

Section: Introductionmentioning

confidence: 99%

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Flexible Pectin Nanopatterning Drives Cell Wall Organization in Plants

Siemianowski,

Rongpipi,

Del Mundo

et al. 2024

JACS Au

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Plant cell walls are abundant sources of materials and energy. Nevertheless, cell wall nanostructure, specifically how pectins interact with cellulose and hemicelluloses to construct a robust and flexible biomaterial, is poorly understood. X-ray scattering measurements are minimally invasive and can reveal ultrastructural, compositional, and physical properties of materials. Resonant X-ray scattering takes advantage of compositional differences by tuning the energy of the incident X-ray to absorption edges of specific elements in a material. Using Tender Resonant Xray Scattering (TReXS) at the calcium K-edge to study hypocotyls of the model plant, Arabidopsis thaliana, we detected distinctive Ca features that we hypothesize correspond to previously unreported Ca-Homogalacturonan (Ca-HG) nanostructures. When Ca-HG structures were perturbed by chemical and enzymatic treatments, cellulose microfibrils were also rearranged. Moreover, Ca-HG nanostructure was altered in mutants with abnormal cellulose, pectin, or hemicellulose content. Our results indicate direct structural interlinks between components of the plant cell wall at the nanoscale and reveal mechanisms that underpin both the structural integrity of these components and the molecular architecture of the plant cell wall.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%