Extracting three-dimensional orientation and tractography of myofibers using optical coherence tomography

Gan, Yu; Fleming, Christine P.

doi:10.1364/boe.4.002150

Cited by 48 publications

(27 citation statements)

References 36 publications

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“…Both samples were taken within 5 mm of the internal os. The OCT signal was conditioned based on a previously published algorithm for heart tissue (Fleming et al, 2008; Gan and Fleming, 2013) and optimized for cervical collagen (Gan et al, 2014). …”

Section: Methodsmentioning

confidence: 99%

A continuous fiber distribution material model for human cervical tissue

Myers

Hendon

Gan

et al. 2015

Journal of Biomechanics

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The uterine cervix during pregnancy is the vital mechanical barrier which resists compressive and tensile loads generated from a growing fetus. Premature cervical remodeling and softening is hypothesized to result in the shortening of the cervix, which is known to increase a woman’s risk of preterm birth. To understand the role of cervical material properties in preventing preterm birth, we derive a cervical material model based on previous mechanical, biochemical and histological experiments conducted on nonpregnant and pregnant human hysterectomy cervical tissue samples. In this study we present a three-dimensional fiber composite model that captures the equilibrium material behavior of the tissue in tension and compression. Cervical tissue is modeled as a fibrous composite material, where a single family of preferentially aligned and continuously distributed collagen fibers are embedded in a compressible neo-Hookean ground substance. The total stress in the collagen solid network is calculated by integrating the fiber stresses. The shape of the fiber distribution is described by an ellipsoid where semi-principal axis lengths are fit to optical coherence tomography measurements. The composite material model is fit to averaged mechanical testing data from uni-axial compression and tension experiments, and averaged material parameters are reported for nonpregnant and term pregnant human cervical tissue. The model is then evaluated by investigating the stress and strain state of a uniform thick-walled cylinder under a compressive stress with collagen fibers preferentially aligned in the circumferential direction. This material modeling framework for the equilibrium behavior of human cervical tissue serves as a basis to determine the role of preferentially-aligned cervical collagen fibers in preventing cervical deformation during pregnancy.

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Section: Methodsmentioning

confidence: 99%

A continuous fiber distribution material model for human cervical tissue

Myers

Hendon

Gan

et al. 2015

Journal of Biomechanics

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“…This method has been previously applied to imaging fiber orientation in OCT intensity images [7–9]. The two-dimensional gradient at each image pixel was first computed using a 3 × 3 Sobel edge detector.…”

Section: Methodsmentioning

confidence: 99%

Histology validation of mapping depth-resolved cardiac fiber orientation in fresh mouse heart using optical polarization tractography

Wang

Zhang

Wasala

et al. 2014

Biomed. Opt. Express

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Myofiber organization in cardiac muscle plays an important role in achieving normal mechanical and electrical heart functions. An imaging tool that can reveal microstructural details of myofiber organization is valuable for both basic research and clinical applications. A high-resolution optical polarization tractography (OPT) was recently developed based on Jones matrix optical coherence tomography (JMOCT). In this study, we validated the accuracy of using OPT for measuring depth-resolved fiber orientation in fresh heart samples by comparing directly with histology images. Systematic image processing algorithms were developed to register OPT with histology images. The pixel-wise differences between the two tractographic results were analyzed in details. The results indicate that OPT can accurately image depth-resolved fiber orientation in fresh heart tissues and reveal microstructural details at the histological level.

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“…This was enabled by an image-stitching algorithm to increase the field of view (FOV), encompassing entire axial slices (~3 cm × 3 cm), which allowed for assessment of collagen fiber orientation trends and for identification of unique anatomical regions. Our previous methods for fiber orientation estimation [24, 25] targeted extracting dominant fiber orientation in a subregion, however it could not provide detailed information about non-dominant orientations, resulting inaccuracy in the evaluation of fiber dispersion.…”

Section: Introductionmentioning

confidence: 99%

Collagen Fiber Orientation and Dispersion in the Upper Cervix of Non-Pregnant and Pregnant Women

et al. 2016

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The structural integrity of the cervix in pregnancy is necessary for carrying a pregnancy until term, and the organization of human cervical tissue collagen likely plays an important role in the tissue’s structural function. Collagen fibers in the cervical extracellular matrix exhibit preferential directionality, and this collagen network ultrastructure is hypothesized to reorient and remodel during cervical softening and dilation at time of parturition. Within the cervix, the upper half is substantially loaded during pregnancy and is where the premature funneling starts to happen. To characterize the cervical collagen ultrastructure for the upper half of the human cervix, we imaged whole axial tissue slices from non-pregnant and pregnant women undergoing hysterectomy or cesarean hysterectomy respectively using optical coherence tomography (OCT) and implemented a pixel-wise fiber orientation tracking method to measure the distribution of fiber orientation. The collagen fiber orientation maps show that there are two radial zones and the preferential fiber direction is circumferential in a dominant outer radial zone. The OCT data also reveal that there are two anatomic regions with distinct fiber orientation and dispersion properties. These regions are labeled: Region 1—the posterior and anterior quadrants in the outer radial zone and Region 2—the left and right quadrants in the outer radial zone and all quadrants in the inner radial zone. When comparing samples from nulliparous vs multiparous women, no differences in these fiber properties were noted. Pregnant tissue samples exhibit an overall higher fiber dispersion and more heterogeneous fiber properties within the sample than non-pregnant tissue. Collectively, these OCT data suggest that collagen fiber dispersion and directionality may play a role in cervical remodeling during pregnancy, where distinct remodeling properties exist according to anatomical quadrant.

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Extracting three-dimensional orientation and tractography of myofibers using optical coherence tomography

Cited by 48 publications

References 36 publications

A continuous fiber distribution material model for human cervical tissue

A continuous fiber distribution material model for human cervical tissue

Histology validation of mapping depth-resolved cardiac fiber orientation in fresh mouse heart using optical polarization tractography

Collagen Fiber Orientation and Dispersion in the Upper Cervix of Non-Pregnant and Pregnant Women

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