Extraction and characterisation of pepsin-solubilised collagen from fins, scales, skins, bones and swim bladders of bighead carp (Hypophthalmichthys nobilis)

Liu, Dasong; Liang, Li; Regenstein, Joe M.; Zhou, Peng

doi:10.1016/j.foodchem.2012.02.032

Cited by 251 publications

(281 citation statements)

References 34 publications

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“…Fish skin collagens are extracted at low temperature (*4°C). The low-temperature extraction process of fish skin collagens were well reported by several researchers (Liu et al 2012;Pal et al 2015). However, authors reported the extraction of collagen at 25-45°C.…”

Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 96%

“…3. It was evident that removal of minerals (demineralization) is a critical step to extract the collagen from the fish scales (Liu et al 2012;Matmaroh et al 2011). However, the author did not remove the minerals from the fish scales and bones.…”

Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 99%

“…Due to the outbreak of the prions disease in the bovine and porcine collagen, most of the research focused on the extraction of the collagen from the alternative sources (fish, marine and seafood sources) Suresh 2016a, b, 2017). There are several research groups which are working on the extraction of the collagen from alternative sources (Liu et al 2012;Matmaroh et al 2011;Pal et al 2016). However, some researchers could not be able to extract the collagen systematically and also published the erroneous information in the reputed international journal.…”

Section: Introductionmentioning

confidence: 99%

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Technical note on the isolation and characterization of collagen from fish waste material

Kumar

Rani

2017

J Food Sci Technol

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The aim of this manuscript was to evaluate the major technical problems on the isolation and characterization of the collagen from fish waste materials that were usually faced by the growing researchers. Although the original research article published by authors contributed new information to the literature, some of them were failed to provide sufficient details in order to reproduce the study as well as could not adequately interpret/compared the results with other publications. Therefore, it is required to research the technical problems during the isolation and characterization of the collagen. This technical note provides the information which is crucial for the reader's and growing researchers for understanding as an essential part of the published research studies about the collagen extraction and characterization. Hence, this technical note may be helpful to those working on the collagen extraction and characterization from fish/marine waste materials.

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Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 96%

Section: Technical Comments On Published Articles On Collagen Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Technical note on the isolation and characterization of collagen from fish waste material

Kumar

Rani

2017

J Food Sci Technol

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“…This fibrous protein can be obtained from different sources and it is the main component of skins, bones and tendons (Ahmad & Benjakul, 2010;Delvin, 2004;Huang, Shiau, Chen, & Huang, 2011;Jongjareonrak, Benjakul, Visessanguan, Nagai, & Tanaka, 2005;Yan, Li, Zhao, & Qin, 2012). At least 29 different types of collagen with a singular amino acid sequence, structure and function have been identified (Li et al, 2013;Liu, Liang, Regenstein, & Zhou, 2012). Collagen is a biopolymer composed of two alpha-1 chains and one alpha-2 chain.…”

Section: Introductionmentioning

confidence: 99%

Extraction and characterization of collagen from rabbit skin: partial characterization

Martínez-Ortiz

Hernández-Fuentes

Pimentel-González

et al. 2014

CyTA - Journal of Food

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(2015) Extraction and characterization of collagen from rabbit skin: partial characterization, CyTAJournal of Food, 13:2, 253-258, DOI: 10.1080/19476337.2014 To link to this article: https://doi.org/10. 1080/19476337.2014 Extraction and characterization of collagen were carried out in rabbit skins as a new alternative for collagen type I. Acetic acid and pepsin were for the extraction of soluble and insoluble collagen, respectively. The enzymatic treatment yielded higher amount of collagen (71%). The average pH value was 6.3, no matter what the method of extraction was. Denaturation temperature of collagen was found at 36°C approximately in two different techniques: Rheometer and Differential Scanning Calorimetry (DSC). Despite the amount of collagen in solution was low, its viscosity was high because of the hydrodynamic behaviour of collagen molecules. Sodium dodecyl sulphate polyacrylamide gel electrophoresis results showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. Determination of hydroxyproline gave evidence that the extracted material was collagen. It was concluded that rabbit skin could be an alternative source for the extraction of collagen.Keywords: collagen; rabbit skin; extraction; thermal analysis Se realizó la extracción y caracterización de colágeno en pieles de conejo como una nueva alternativa para colágeno tipo I. Se usaron ácido acético y pepsina para la extracción de colágeno soluble e insoluble respectivamente. El tratamiento enzimático fue el que rindió alta cantidad de colágeno (71%). El pH promedio fue de 6,3 sin importar cuál fue el método de extracción. La temperatura de desnaturalización del colágeno se encontró a 36°C aproximadamente mediante dos diferentes técnicas: Reometría y DSC. A pesar de que la cantidad de colágeno en la solución fue bajo, su viscosidad fue alta debido a el comportamiento hidrodinámico de las moléculas. Los resultados de SDS-PAGE mostraron tres diferentes bandas en las que se reflejaron dos cadenas alfa y una cadena beta con pesos moleculares de 102, 118 and 220 kDa respectivamente. La determinación del contenido de hidroxiprolina dio evidencia de que el material extraído fue colágeno. Se concluyó que la piel de conejo podría ser una alternativa de materia prima para la extracción de colágeno.

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“…It has been widely consumed in more than 70 countries around the world (Liu et al 2012). Therefore, in this study, gelatin was extracted from bighead carp scales by ultrasound-assisted extraction.…”

Section: Introductionmentioning

confidence: 99%

Physico-chemical properties of gelatin from bighead carp (Hypophthalmichthys nobilis) scales by ultrasound-assisted extraction

et al. 2013

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In this study, gelatin was extracted from bighead carp (Hypophthalmichthys nobilis) scales by water bath (WB) and ultrasound bath (UB) at 60°C for 1 h, 3 h and 5 h, named WB1, WB3, WB5, UB1, UB3 and UB5, respectively. The physicochemical properties of gelatin were investigated. The result indicated that gelatin extracted from bighead carp scales had a high protein content (84.15~91.85 %) with moisture (7.111 3.65 %), low ash content (0.31~0.97 %). All extracted gelatin contained α-and β-chains as the predominant components. Gelatin extracted by UB obtained much higher yield (30.94-46.67 %) than that of WB (19.15-36.39 %). More voids and less sheets of gelatin structure were observed, when the gelatin was extracted by UB for longer time. Gelatin of UB-assisted extraction normally exhibited lower gel strength and melting points than that of WB, which may be resulted from the protein degradation reflected by the FTIR spectra and higher free amino group content. However, there was no significant difference between WB1 and UB1. Therefore, the ultrasound assisted extraction in a short time was a promising method to enhance the yield and obtain gelatin with high quality.

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Extraction and characterisation of pepsin-solubilised collagen from fins, scales, skins, bones and swim bladders of bighead carp (Hypophthalmichthys nobilis)

Cited by 251 publications

References 34 publications

Technical note on the isolation and characterization of collagen from fish waste material

Technical note on the isolation and characterization of collagen from fish waste material

Extraction and characterization of collagen from rabbit skin: partial characterization

Physico-chemical properties of gelatin from bighead carp (Hypophthalmichthys nobilis) scales by ultrasound-assisted extraction

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