Extraction and determination of enzymes produced by Ceriporiopsis subvermispora during biopulping of Pinus taeda wood chips

Souza-Cruz, Priscila Brasil de; Freer, Juanita; Siika‐aho, Matti; Ferraz, André

doi:10.1016/j.enzmictec.2003.10.005

Cited by 99 publications

(80 citation statements)

References 25 publications

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“…It was also observed that laccase by C. subvermispora and P. acerina may play a major role in lignin degradation. The result differed from the production of ligninolytic enzymes in the wood culture of C. subvermispora, which indicated that MnP was the main ligninolytic enzyme during the fungal cultivation with less or no laccase detected (Souza-Cruz et al 2004). This discrepancy may be due to the differences in culture conditions.…”

Section: Fig 2 Manganese Peroxidase Activity Of Different Fungi Durmentioning

confidence: 62%

Effect of Fungal Treatments of Rape Straw on Chemical Composition and in vitro Rumen Fermentation Characteristics

Zhao¹,

Gong²,

Zhou³

et al. 2014

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The use of rape straw in ruminant production is limited by its high lignin content and low ruminal degradability. White rot fungi are the most efficient known degraders of lignin. Four white rot fungi were investigated for their potential to degrade lignin and improve rumen fermentation of rape straw. Solid state fermentation of the straw was carried out for 0 to 30 days to determine changes in chemical composition and in vitro rumen fermentation. Results showed that Phanerochaete chrysosporium and Lentinula edodes degraded about 45% of lignin and enhanced the in vitro organic matter digestibility (IVOMD) and volatile fatty acid production; however, about 55% of the cellulose was lost after 30 days of incubation. Ceriporiopsis subvermispora and Phlebia acerina degraded a fraction (< 30%) of lignin and cellulose, but inhibited ruminal fermentation. Fungal incubation increased the chitin content of rape straw. Regression analysis showed that the IVOMD increase depended on the combined action of neutral detergent fiber loss and chitin content increase in rape straw. This study indicates that considerations of the conversion of rape straw into ruminant feed with white-rot fungi should take into account the degradation of lignin, fiber loss, and the chitin produced along with the growth of fungi.

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Section: Fig 2 Manganese Peroxidase Activity Of Different Fungi Durmentioning

confidence: 62%

Effect of Fungal Treatments of Rape Straw on Chemical Composition and in vitro Rumen Fermentation Characteristics

Zhao¹,

Gong²,

Zhou³

et al. 2014

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“…In the SSF cultures, three biological replicate experiments (parallel cultures) per strain per time point were used. In this case, the enzymes released to the medium were extracted from the solid culture by adding 40 ml of sodium acetate buffer (50 mM, pH 5.0) and shaking the extraction mixtures at 150 rpm for 4 h at 10°C as described by de Souza-Cruz et al (5). Following the extraction, the liquid fraction was collected and centrifuged to remove debris, and the subsequent supernatant was used for enzyme determina-tion.…”

Section: Methodsmentioning

confidence: 99%

Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Castanera

Pérez

Omarini

et al. 2012

Appl Environ Microbiol

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ABSTRACTThe genome of the white rot basidiomycetePleurotus ostreatusincludes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) theLacc2andLacc10genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and theLaccgene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.

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“…Endoglucanases are produced by fungus in the first incubation. In the second incubation, no fungal biomass was used to avoid incorporating the glucose generated (Brasil et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill

Ordaz-Díaz¹,

Rojas‐Contreras

Flores-Vichi³

et al. 2016

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The aim of this study was to identify cellulolytic fungal strains capable of degrading cellulose from an aerated lagoon in a pulp and paper mill. Four fungal strains that were found to be highly active were isolated on carboxymethyl cellulose (CMC) and suggested to be CMCase/endoglucanase. The identified strains were Aspergillus niger, Penicillium sp., Aspergillus fumigatus, and Mucor sp. All the strains were studied in terms of cultural morphological characteristics and microscopic examinations. The endoglucanase with the highest isolate production was Penicillium sp., which also showed the highest qualitative endoglucanase activity (1.3 cm), in addition to the main activity of endoglucanase with 297 mmol/mg.min after 116 h. The results indicated that CMC is able to induce endoglucanase enzyme production and that the fungal isolates showed significant cellulose degradation properties.

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Extraction and determination of enzymes produced by Ceriporiopsis subvermispora during biopulping of Pinus taeda wood chips

Cited by 99 publications

References 25 publications

Effect of Fungal Treatments of Rape Straw on Chemical Composition and in vitro Rumen Fermentation Characteristics

Effect of Fungal Treatments of Rape Straw on Chemical Composition and in vitro Rumen Fermentation Characteristics

Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill

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