Extraction and preconcentration of hemin from human blood serum and breast cancer supernatant

Abstract

A green, facile, fast, and sensitive liquid-phase microextraction method is presented for the extraction and preconcentration of hemin in the presence of free iron ions prior to flame atomic absorption spectroscopic determination. In this technique, an anion-functionalized task-specific ionic liquid is used as the extracting solvent. The interface between the extracting solvent and the bulk aqueous phase containing hemin is enormously enlarged by dispersing the ionic liquid to the aqueous phase with the help of ultrasound radiation. Hemin is selectively extracted into the ionic liquid after interaction with the functional group of the ionic liquid. Then, the concentration of the extracted hemin is determined through the absorbance of the iron ions contained in the hemin structure using flame atomic absorption spectroscopy. Different experimental parameters affecting the extraction efficiency have been optimized. Under the optimized conditions, the proposed method has a hemin concentration linear range of 0.020-0.80 mg/L with a detection limit of 0.0080 mg/L. This method has been successfully applied to the extraction and determination of hemin in human serum and supernatant samples.

Effect of the Hemin Molecular Complexes on the Structure and Properties of the Composite Electrospun Materials Based on Poly(3-hydroxybutyrate)

“…There is a large number of works that show the effectiveness of hemin as a binding peptides element used for medical purposes [54,55]. The low cost of hemin extraction and purification makes it even more attractive for research purposes and further industrial translation [56].…”

Recent Advances in the Design and Sensing Applications of Hemin/Coordination Polymer‐Based Nanocomposites

“…[ 13,34 ] iii) Cost‐effectiveness: the low cost of hemin extraction and purification makes it ideal for research purposes and further industrial translation. [ 35,36 ]…”

Highly Scalable Conversion of Blood Protoporphyrin to Efficient Electrocatalyst for CO2‐to‐CO Conversion

“…[ 34 ] From the average blood volume in cattle (60 mL Kg −1 ) and pigs (62 mL kg −1 ), [ 35 ] the annual worldwide animal blood production can be determined to be 1.4 × 10 10 L for cattle and 8.9 × 10 9 L for pigs. Using either of the two published methodologies for hemin extraction from animal blood with 0.75 mg L −1 (trioctylmethyl ammonium salicylate) [ 36 ] or 10 g L −1 (acetic acid/acetone/HCl), [ 37 ] thus the potential yearly hemin production to be 1.7 × 10 4 or 2.2 × 10 8 kg can be calculated, respectively.…”