Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems

Abstract

Extrato aquoso de C-ficocianina de Spirulina platensis, com e sem resíduos celulares, foi purificado em sistema aquoso bifásico (SAB) polietilenoglicol (PEG)/fosfato de potássio variando-se a massa molar do PEG, a pH 6, mais promissor do que a pH 7. Para os ensaios realizados com cada massa molar, com diferentes percentuais de PEG e sal, as concentrações (mg mL -1 ), as purezas (OD 620/OD 280) e os coeficientes de partição da C-ficocianina de cada sistema foram determinados e comparados. O processo para a purificação primária de C-ficocianina intracelular com resíduos celulares (integrado), composto por 15% de PEG 1500 e 13% de fosfato de potássio de 5% de PEG 4000 e 18% de sal, alcançaram concentrações de C-ficocianina de, respectivamente, 1,60 e 2,67 mg mL -1 na fase de topo. As purezas foram de 0,73 e 0,79, respectivamente, valores superiores aos obtidos nos sistemas sem resíduos celulares.Crude C-phycocyanin extracts from Spirulina platensis, with and without cell debris, were purified in aqueous two-phase systems (ATPS) varying the molar mass of the PEG, at pH 6, more promising than at pH 7. For the assays carried out with each molar mass, with distinct percentages of PEG and salt, the concentrations (mg mL -1 ), purities (OD 620/OD 280) and C-phycocyanin partition coefficients of each system were determined and compared. The process for the primary purification of intracellular C-phycocyanin with cell debris (integrated), composed of 15% of PEG 1500 and 13% of potassium phosphate and 5% of PEG 4000 and 18% of salt, produced C-phycocyanin concentrations of, respectively, 1.60 and 2.67 mg mL -1 in the top phase. The purities were of 0.73 and 0.79 respectively, values higher than those obtained in the systems without cells.

Effects of different drying methods on C-phycocyanin content of Spirulina platensis powder

“…The purity of C-PC preparations was evaluated based on the ratio between absorbencies from phycocyanobilin at 620 nm and aromatic amino acids in all proteins in the preparation at 280 nm (Antelo et al, 2010).…”

“…The purity of the phycocyanin is an important factor that determines its application area. The C-phycocyanin purity ratio is considered as the food grade when A620 / A280 is ≥ 0.7, and as the reagent grade when A620 / A280 is between 0.7 and 3.9; and as an analytical grade when A620 / A280 is ≥ 4.0 (Antelo et al, 2010;Kuddus et al, 2013). Food-grade phycocyanin is utilized safely as a dye in the food formulation without offering health risk (Kim et al, 2008).…”

Aplicações Ambientais De Microalgas

“…As microalgas tem sido foco de inúmeras investigações biotecnológicas devido a sua importância econômica, nutricional e ecológica (ANTELO et al, 2010). Nesse tópico, estão apresentadas algumas das aplicações ambientais das microalgas.…”

Use of Solid Waste from Thermoelectric Plants for the Cultivation of Microalgae

“…One alternative to combat this type of pollution is the use of biological processes (Schmitz et al 2012). The use of microalgae is one such biological process with economic, nutritional and ecological importance due to a microalga's capacity to double its biomass within 24 h (Becker 2004;Antelo et al 2010). Microalgae are capable of removing heavy metals from the environment due to their active, or mediating ability in mobilization, or immobilization processes that influence the equilibrium of metallic species between the solid and liquid phases.…”