Extraction and Recovery of Rutin from Acerola Waste using Alcohol-Salt-Based Aqueous Two-Phase Systems

Abstract

Extraction of rutin from acerola waste was investigated using alcohol-salt-based aqueous two-phase systems (ATPS). Initially, the partitioning was studied using model systems with pure and commercial rutin. The impact of the ATPS constituents and composition, initial amount of rutin, temperature and addition of electrolytes was evaluated. Rutin can be recovered either in the alcohol-or-salt-rich phase depending on the salt used. To validate the optimization process, rutin extraction from acerola waste was carried out further. The results obtained with the real samples are in close agreement with the model systems and validate the optimization tests and support their applicability in bioresourcerelated processes.

Novel aspects and future trends in the use of aqueous two‐phase systems as a bioengineering tool

“…Interestingly, alcohol–salt ATPS are mostly used in the recovery and fractionation of high‐value products from economical sources and even waste streams probably due to the fact that these phase‐forming chemicals are inexpensive and may lower production costs when scaled efficiently . For instance, antibiotics have been recovered in ethanol/2‐propanol – ammonium sulfate ATPS; and anthraquinone derivatives and rutin have also been recuperated from Aloe vera and acerola wastes respectively . Nonetheless, macromolecules such as serine proteases and interpheron α‐2b have been recently recovered with recovery yields higher than 75% (with NaCl addition) in these type of systems showing that appropriate design parameters may avoid the usual complications associated with macromolecule recovery.…”

Recovery of phenolic compounds from Eucalyptus wood wastes using ethanol-salt-based aqueous two-phase systems

“…Particulary, ATPS formed by alcohol and salts are of low cost and allow an easy recovery of the alcohol by evaporation or distillation (Reis et al 2014). Moreover, alcohol-salt ATPS are of low viscosity, and usually led to high extraction efficiencies and purification levels in a single-step procedure (Reis et al 2014). The low viscosity is industrially relevant as the cost of pumping fluids is reduced (Simental-Martínez et al 2014).…”

“…These systems have some drawbacks such as the high cost of polymers, the low segregation of the phases and the complications associated with recycling components lar for recovery of biomolecules have been studied (Espitia-Saloma et al 2014). Particulary, ATPS formed by alcohol and salts are of low cost and allow an easy recovery of the alcohol by evaporation or distillation (Reis et al 2014). Moreover, alcohol-salt ATPS are of low viscosity, and usually led to high extraction efficiencies and purification levels in a single-step procedure (Reis et al 2014).…”

“…ATPS composed of an organic solvent and an inorganic salt have been used to extract anthocyanins (Wu et al 2014), rutin (Reis et al 2014), lipase (Ooi et al 2009), fucoxanthin (Gómez-Loredo et al 2014, ascorbic acid (Reis et al 2012), among others. However, at present, there is no literature regarding the partition behaviour of phenols applying alcohol-salt ATPS directly over a raw material without purification.…”

“…Aqueous two-phase system (ATPS) extraction is a liquid-liquid extraction technique that has been used for the extraction and partial purification of a wide variety of biomolecules (Rosa et al 2010, Espitia-Saloma et al 2014, Reis et al 2014, Wu et al 2014. ATPS form when an aqueous solution exceeds a critical concentration of two water soluble, but mutually incompatible, components (Albertsson 1986).…”