A single-step selective separation of two food additives was investigated using alcohol-salt aqueous two-phase systems (ATPS). The selective partitioning of two of the most used additives from a processed food waste material, vanillin and l-ascorbic acid, was successfully accomplished. The results obtained prove that alcohol-salt ATPS can be easily applied as cheaper processes for the selective recovery of valuable chemical products from food wastes and other sources. As a first approach, the phase diagrams of ATPS composed of different alcohol+inorganic salt+water were determined at 298 (± 1)K and atmospheric pressure. The influence of methanol, ethanol, 1-propanol, and 2-propanol and K(3)PO(4), K(2)HPO(4) or KH(2)PO(4)/K(2)HPO(4) in the design of the phase diagrams was addressed. After the evaluation of the phase diagrams behaviour, the influence of the phase forming constituents was assessed towards the partition coefficients and recovery percentages of vanillin and l-ascorbic acid among the coexisting phases. Both model systems and real processed food waste materials were employed. Using these ATPS as partitioning systems it is possible to recover and separate vanillin, which migrates for the alcohol-rich phase, from l-ascorbic acid, which preferentially partitions for the salt-rich phase.

Extraction of rutin from acerola waste was investigated using alcohol-salt-based aqueous two-phase systems (ATPS). Initially, the partitioning was studied using model systems with pure and commercial rutin. The impact of the ATPS constituents and composition, initial amount of rutin, temperature and addition of electrolytes was evaluated. Rutin can be recovered either in the alcohol-or-salt-rich phase depending on the salt used. To validate the optimization process, rutin extraction from acerola waste was carried out further. The results obtained with the real samples are in close agreement with the model systems and validate the optimization tests and support their applicability in bioresourcerelated processes.

This work proposes the application of flexible alcoholic aqueous two-phase systems (ATPS) to manipulate the partition of caffeine. An optimization study was performed the caffeine partitioning towards top phase (60.0 wt% 2-propanol + 20.0 wt% K 3 PO 4 at 303 K -K caf = 3.4 and R T =89.1%) and bottom phase (60.0 wt% methanol + 17.5 wt% K 2 HPO 4 /KH 2 PO 4 at 278 K -K caf = 0.06 and R B = 81.1%). These processes were evaluated by applying them to real biomass as guaraná seeds (purification factor -FP = 6.59 fold) and coffee beans (PF = 3.24 fold) for the 2-propanol and K 2 HPO 4 /KH 2 PO 4 system.

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Abarema cochliacarpos é uma espécie endêmica do Brasil, pertence à família Fabaceae e é conhecida popularmente como barbatimão, estando presente na caatinga e cerrado brasileiro. Em Sergipe, seu uso é em forma de chás para atividades anti-inflamatória e cicatrizante. Como objetos deste estudo foram utilizados as folhas do vegetal a fim de conhecer qualitativamente os constituintes químicos das frações obtidas a partir do extrato bruto: fração clorofórmica (FCL), fração hexânica (FHX), fração acetato de etila (FAE) e fração hidrometanólica (FHM) através da prospecção fitoquímica por ensaios colorimétricos, sendo detectada a presença de metabólitos secundários como fenóis, taninos, flavonóis, catequinas, esteroides, triterpenóides e saponinas. Na análise antimicrobiana apenas duas frações apresentaram halos de inibição. A FAE para as cepas de Staphylococcus aureus (19,3 mm), Enterococcus durans hirae (10 mm), Escherichia coli derivada (9,6 mm) e Pseudomonas aeruginosa derivada (15 mm), e a FCL para a cepa E. durans hirae (9 mm). Na concentração inibitória mínima (MIC) a FAE sobressaiu-se com 12,5 µg.mL-1 para a S. aureus e E. durans, enquanto a FCL obteve 25 µg.mL-1 para E. durans. Quanto à análise citotóxica, a FAE nas concentrações testadas não favoreceu a viabilidade celular em 75%, apresentando capacidade antiproliferativa, exceto a FLC a 20 µg.mL-1. A FAE e FCL apresentaram propriedades antioxidantes e obtiveram efeito antimicrobiano, além de possuírem metabólitos que corroboram com essas funções.