Extraction conditions of Antheraea mylitta sericin with high yields and minimum molecular weight degradation

Yun, Haesung; Oh, Han-Jin; Kim, Moo Kon; Kwak, Hyo Won; Lee, Jeong Yun; Um, In Chul; Vootla, Shyam Kumar; Lee, Ki Hoon

doi:10.1016/j.ijbiomac.2012.09.017

Cited by 52 publications

(22 citation statements)

References 22 publications

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“…The silk mats were prepared as described by Armato 59 and stored in sterile distilled water. Briefly, Am and Bm mats were prepared separately by degumming to remove the sericin by alkaline method (0.02 M Na 2 CO 3 ) and dried in an oven at 50 °C for 3 days 60 . The mats of approximately 1 cm × 1 cm pieces were sterilized in 70% ethanol for 15 min followed by rinsing in sterile phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

Physico-chemical characterization of Antheraea mylitta silk mats for wound healing applications

Darshan

Kong

Gautrot

et al. 2017

Sci Rep

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In the field of plastic reconstructive surgery, development of new innovative matrices for skin repair is in demand. The ideal biomaterial should promote attachment, proliferation and growth of cells. Additionally, it should degrade in an appropriate time period without releasing harmful substances, not exerting a pathological immune response. The materials used should display optimized mechanical properties to sustain cell growth and limit scaffold contraction. Wound healing is a biological process directed towards restoration of tissue that has suffered an injury. An important phase of wound healing is the generation of a basal epithelium wholly replacing the epidermis of the wound. Wild silk from Antheraea mylitta meets these demands to a large extent. To evaluate the effects of the treatment, Antheraea mylitta and Bombyx mori samples were characterized by SEM-EDX, FT-IR, XRD and TGA-DSC techniques. Preliminary cell growth behavior was carried out by culturing epidermal cells and proliferation was quantified via viability assay. Moreover, Antheraea mylitta possesses excellent cell-adhesive capability, effectively promoting cell attachment and proliferation. Antheraea mylitta serves as a delivery vehicle for cells. With all these unique features, it is expected that Antheraea mylitta mat will have wide utility in the areas of tissue engineering and regenerative medicine.

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Section: Methodsmentioning

confidence: 99%

Physico-chemical characterization of Antheraea mylitta silk mats for wound healing applications

Darshan

Kong

Gautrot

et al. 2017

Sci Rep

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“…13 SF solution can be fabricated into films, particles, electrospun fibers, nets, sponges, hydrogels, and threedimensional (3D) porous scaffolds. [14][15][16][17][18] They have been widely used in musculoskeletal, vascular, skin, and nerve tissue engineering. Pictures of different morphologies of silk-based biomaterials for MTE are shown in Figure 1.…”

Section: Morphological Diversification Of Silk Scaffolds For Mtementioning

confidence: 99%

“…Moreover, studies on other sources of SF such as non-mulberry and spider silk have got increased attention in the recent years. 17 The present review is focused on recent researches about silk-based materials in the area of musculoskeletal tissue regeneration. Different forms of silk scaffolds are discussed, followed by their applications in bone, cartilage, tendon/ ligament, and skeletal muscle regeneration.…”

Section: Introductionmentioning

confidence: 99%

Silk scaffolds for musculoskeletal tissue engineering

Yao

Liu

Fan

2015

Exp Biol Med (Maywood)

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The musculoskeletal system, which includes bone, cartilage, tendon/ligament, and skeletal muscle, is becoming the targets for tissue engineering because of the high need for their repair and regeneration. Numerous factors would affect the use of musculoskeletal tissue engineering for tissue regeneration ranging from cells used for scaffold seeding to the manufacture and structures of materials. The essential function of the scaffolds is to convey growth factors as well as cells to the target site to aid the regeneration of the injury. Among the variety of biomaterials used in scaffold engineering, silk fibroin is recognized as an ideal material for its impressive cytocompatibility, slow biodegradability, and excellent mechanical properties. The current review describes the advances made in the fabrication of silk fibroin scaffolds with different forms such as films, particles, electrospun fibers, hydrogels, three-dimensional porous scaffolds, and their applications in the regeneration of musculoskeletal tissues.

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“…time is increased, more sericin can be extracted, but at the same time, the sericin molecules become more degraded. Considering that many studies have been conducted to improve the mechanical properties of sericin and to extract high molecular weight (MW) sericin (Oh et al, 2007(Oh et al, , 2011Yun et al, 2013), it is clearly desirable to avoid the severe molecular degradation of silk sericin during the extraction process. That is, it is important to properly control the extraction time of sericin in hot water to simultaneously obtain both a high yield and a high MW.…”

Section: Production Yield and Amino Acid Compositionmentioning

confidence: 99%

Effect of Extraction Time on the Rheological Properties of Sericin Solutions and Gels

Yoo¹,

Um²

2013

International Journal of Industrial Entomology

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Recently, silk sericin has attracted the attention of researchers owing to its useful properties as a biomaterial including 1) good wound healing and cell activities, 2) fast gelation character, and 3) high water retention property. In the present study, silk sericin was prepared using different extraction times in hot water and the effect of extraction time on the rheological properties of sericin solutions and gels was examined. It was found that the production yield of sericin increased with extraction time. The shear viscosity of sericin solutions and gels decreased with increasing extraction time due to a decrease in sericin molecular weight.When the sericin solution transformed to a gel, the viscosity increased and the shear thinning behavior was more evident. In addition, the shear stress measurements indicated that the slip between the sericin samples and the measuring plate of the rheometer was increased by the gelation of sericin. The compression strength of sericin gel could be increased remarkably (by more than 100 fold) by preparation using the freezing and thawing method.

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Extraction conditions of Antheraea mylitta sericin with high yields and minimum molecular weight degradation

Cited by 52 publications

References 22 publications

Physico-chemical characterization of Antheraea mylitta silk mats for wound healing applications

Physico-chemical characterization of Antheraea mylitta silk mats for wound healing applications

Silk scaffolds for musculoskeletal tissue engineering

Effect of Extraction Time on the Rheological Properties of Sericin Solutions and Gels

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